Differential Expression of p16INK4A and Cyclin D1 in Benign and Malignant Salivary Gland Tumors: A Study of 44 Cases

Jour, George; West, Kelsey; Ghali, V. S.; Shank, Deborah L.; Ephrem, Georges; Wenig, Bruce M.

doi:10.1007/s12105-012-0417-9

Cited by 37 publications

(28 citation statements)

References 14 publications

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“…Indeed, E6/E7 mRNA viral transcripts were not detected in any of the 92 MECs evaluated including those 14 tumors that were known not to harbor a driver translocation involving MAML2. The absence of high risk HPV using an RNA in situ hybridization approach concurs with its reported absence in those studies using a DNA in situ hybridization approach [15,19]. Outlier studies may reflect, in part, differences in the ability to distinguish between biologically relevant and irrelevant HPV based on methodologies and test interpretation.…”

Section: Discussionsupporting

confidence: 67%

“…reported that about half of all MECs are infected with transcriptionally active high risk HPV, an incidence that rivals its occurrence in the oropharynx. In an effort to substantiate this finding, Jour et al [15] were not able to detect HPV in any of their MECs, but their number of cases was limited (n = 14) and HPV detection was restricted to a DNA in situ hybridization approach. Our study was designed to help resolve the uncertainty surrounding the potential role of HPV in MECs.…”

Section: Discussionmentioning

confidence: 99%

“…In this study, p16 immunohistochemical staining was not used because p16 staining has been found to be a very poor surrogate marker for the presence of high risk HPV when dealing with salivary gland tumors [15,16]. In situ hybridization for HPV E6/E7 mRNA was performed manually using the RNAscope kit (Advanced Cell Diagnostics Inc., Hayward, CA, USA).…”

Section: Maml2 Fluorescence In Situ Hybridizationmentioning

confidence: 99%

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Mucoepidermoid Carcinoma Does Not Harbor Transcriptionally Active High Risk Human Papillomavirus Even in the Absence of the MAML2 Translocation

Bishop

Yonescu

Batista

et al. 2014

Head and Neck Pathol

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High risk human papillomavirus (HPV) is firmly established as an important cause of oropharyngeal carcinoma. Recent studies have also implicated HPV as a cause of mucoepidermoid carcinoma (MEC)-a tumor of salivary gland origin that frequently harbors MAML2 translocations. The purpose of this study was to determine the prevalence of transcriptionally active HPV in a large group of MECs and to determine whether HPV infection and the MAML2 translocation are mutually exclusive events. Break-apart fluorescence in situ hybridization for MAML2 was performed on a tissue microarray containing 92 MECs. HPV testing was performed using RNA in situ hybridization targeting high risk HPV mRNA E6/E7 transcripts. Of the 71 MECs that could be evaluated by FISH, 57 (80 %) harbored the MAML2 rearrangement. HPV was not detected in any of the 57 MECs that contained a MAML2 rearrangement, in any of the 14 MECs that did not contain the rearrangement, or in any of the 21 MECs where MAML2 status was unknown. High risk HPV does not appear to play any significant role in the development of MEC. It neither complements nor replaces MAML2 translocation in the tumorigenesis of MEC.

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Section: Discussionsupporting

confidence: 67%

Section: Discussionmentioning

confidence: 99%

Section: Maml2 Fluorescence In Situ Hybridizationmentioning

confidence: 99%

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Mucoepidermoid Carcinoma Does Not Harbor Transcriptionally Active High Risk Human Papillomavirus Even in the Absence of the MAML2 Translocation

Bishop

Yonescu

Batista

et al. 2014

Head and Neck Pathol

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“…In surgical specimens of HNSCC, p16 IHC has been shown to be highly sensitive (approaching 100%) for high‐risk HPV, but the specificity is approximately 80% . The decreased specificity can be problematic as p16 overexpression has been noted in a subset of non‐HPV‐associated HNSCCs, and other tumors such as high grade neuroendocrine carcinoma (small cell carcinoma), sinonasal undifferentiated carcinoma, and adenoid cystic carcinoma . In addition, p16 can be expressed in normal tonsillar crypt epithelium and in the epithelium of benign cysts (branchial cleft cysts, lymphoepithelial cyst) …”

Section: P16 Immunohistochemistrymentioning

confidence: 99%

Fine‐needle aspiration biopsy of HPV‐related squamous cell carcinoma of the head and neck: Current ancillary testing methods for determining HPV status

Bernadt

Collins

2017

Diagnostic Cytopathology

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Human papillomavirus (HPV)-related head and neck squamous cell carcinoma (HNSCC) is a unique form of carcinoma that largely arises from the tonsillar tissue in the oropharynx. These tumors often present with cervical lymphadenopathy resulting in a fine needle aspiration (FNA) biopsy. Use of the cytology specimen to determine the HPV-status has significant prognostic and treatment implications as HPV-related tumors have a more favorable prognosis and response to nonsurgical therapies. While several different ancillary testing methods are available that have proven effective for determining HPV status in FNA specimens from HNSCCs, there is currently no consensus regarding HPV testing in this setting. Diagn. Cytopathol. 2017;45:221-229. © 2016 Wiley Periodicals, Inc.

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“…[6] Proliferation cell nuclear antibody (PCNA) and Cyclin D1 are proliferation markers that are overexpressed in neoplasms, [7][8][9] and their expression is related to the prognosis.…”

Section: Introductionmentioning

confidence: 99%

Correlation between Campanacci’s radiological classification of giant cell tumor of bone and expression of Cyclin D1 and PCNA

Estrada-Villaseñor

Alberto

Bandala

et al. 2017

JST

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Giant cell tumor of bone is considered by his behavior a benign but aggressive neoplasm. The objective of our study was to determine if there is a correlation between the Campanacci's radiological classification of giant cell tumors of bone and the expression by immunohistochemistry of Cyclin D1 and proliferation cell nuclear antibody (PCNA). A retrospective and descriptive study was made. In total, there were 27 cases. All cases showed Cyclin D1 and PCNA positivity. Rho Spearman for Campanacci and Cyclin D1 expression was 0.06 and for Campanacci and PCNA was 0.418. We conclude that there is a positive correlation between PCNA expression in giant cell tumors of Bone and the Campanacci's radiological classification II and III, but Cyclin D1 expression was no related with radiologic features.

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Differential Expression of p16INK4A and Cyclin D1 in Benign and Malignant Salivary Gland Tumors: A Study of 44 Cases

Abstract: Salivary gland tumors (SGT) are a heterogeneous group of lesions. There is conflicting data concerning the molecular events involving the tumour suppressor retinoblastoma protein (pRb)

Cited by 37 publications

References 14 publications

Mucoepidermoid Carcinoma Does Not Harbor Transcriptionally Active High Risk Human Papillomavirus Even in the Absence of the MAML2 Translocation

Mucoepidermoid Carcinoma Does Not Harbor Transcriptionally Active High Risk Human Papillomavirus Even in the Absence of the MAML2 Translocation

Fine‐needle aspiration biopsy of HPV‐related squamous cell carcinoma of the head and neck: Current ancillary testing methods for determining HPV status

Correlation between Campanacci’s radiological classification of giant cell tumor of bone and expression of Cyclin D1 and PCNA

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