Differential Expression of miR-499 and Validation of Predicted Target Genes in the Testicular Tissue of Swine at Different Developmental Stages

Zhang, Xiaojun; Li, Chuanmin; Liu, Xin; Lu, Chao; Bai, Chen; Zhao, Zhihui; Sun, Boxing

doi:10.1089/dna.2014.2728

Cited by 9 publications

(4 citation statements)

References 22 publications

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“…miR-375 has been suggested to play an important role in testicular differentiation in Xenopus [ 70 ]. As in tilapia, miR-499 is also a dominant miRNA in swine, indicating its involvement in testicular development [ 71 ]. miR-9 and miR-192 are also upregulated in tilapia ovary, while miR-27d, miR-29b, miR-92b, miR-144-5p and miR-455 show the same pattern of greater expression in testes at later stage [ 43 ], suggesting they might play crucial roles in tilapia sex differentiation.…”

Section: Discussionmentioning

confidence: 99%

Integrated analysis of miRNA and mRNA expression profiles in tilapia gonads at an early stage of sex differentiation

et al. 2016

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BackgroundMicroRNAs (miRNAs) represent a second regulatory network that has important effects on gene expression and protein translation during biological process. However, the possible role of miRNAs in the early stages of fish sex differentiation is not well understood. In this study, we carried an integrated analysis of miRNA and mRNA expression profiles to explore their possibly regulatory patterns at the critical stage of sex differentiation in tilapia.ResultsWe identified 279 pre-miRNA genes in tilapia genome, which were highly conserved in other fish species. Based on small RNA library sequencing, we identified 635 mature miRNAs in tilapia gonads, in which 62 and 49 miRNAs showed higher expression in XX and XY gonads, respectively. The predicted targets of these sex-biased miRNAs (e.g., miR-9, miR-21, miR-30a, miR-96, miR-200b, miR-212 and miR-7977) included genes encoding key enzymes in steroidogenic pathways (Cyp11a1, Hsd3b, Cyp19a1a, Hsd11b) and key molecules involved in vertebrate sex differentiation (Foxl2, Amh, Star1, Sf1, Dmrt1, and Gsdf). These genes also showed sex-biased expression in tilapia gonads at 5 dah. Some miRNAs (e.g., miR-96 and miR-737) targeted multiple genes involved in steroid synthesis, suggesting a complex miRNA regulatory network during early sex differentiation in this fish.ConclusionsThe sequence and expression patterns of most miRNAs in tilapia are conserved in fishes, indicating the basic functions of vertebrate miRNAs might share a common evolutionary origin. This comprehensive analysis of miRNA and mRNA at the early stage of molecular sex differentiation in tilapia XX and XY gonads lead to the discovery of differentially expressed miRNAs and their putative targets, which will facilitate studies of the regulatory network of molecular sex determination and differentiation in fishes.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-2636-z) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Integrated analysis of miRNA and mRNA expression profiles in tilapia gonads at an early stage of sex differentiation

et al. 2016

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“…QKI can express four subtypes during the development of chicken sperm (Mezquita et al 1998), and quaking (qkv mutant) mice also show severe https://doi.org/10.17221/158/2018-CJAS sperm maturation disorders. In addition, the study showed that QKI expression is increasing in 1-to 7-month-old boar's testes (Zhang et al 2015). These results indicate that QKI plays an important role in the development of testes and in spermatogenesis.…”

Section: Discussionmentioning

confidence: 71%

Apoptosis of porcine Sertoli cells is inhibited by QKI-5 via regulating CASP8

Liang¹,

Liu²,

Guo³

et al. 2019

CZECH J ANIM SCI

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QKI, a KH domain containing RNA binding, is an RNA-binding protein that is involved in cell proliferation and apoptosis through binding to the QKI response element (QRE) site of its target mRNA. And Caspase 8 (CASP8) and Caspase 3 (CASP3) play important roles in the pathway of apoptosis. The purpose of this study was to investigate the effect of QKI-5 on the apoptosis of Sertoli cells. The experimental results show that pig tissues contain QKI-5, QKI-6 and QKI-7. Overexpression of QKI-5 significantly decreased the mRNA expression of CASP8 (P < 0.05) and the protein expression of CASP8 (P < 0.05). On the contrary, inhibiting QKI-5 increased the expression of CASP8 significantly. Overexpression of QKI-5 significantly reduced the apoptosis of Sertoli cells and promoted cell growth (P < 0.05). Furthermore, QKI-5 specifically reduced the stability of CASP8 mRNA by binding QRE sites on CASP8. Our experiments provide preliminary evidence that QKI-5 induces Sertoli cells proliferation by inhibiting apoptosis, and this may be one of the factors promoting testicular development.

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“…Our previous study and that of others showed that miR-499 exhibits a higher expression level during the fetal and prepubertal periods than in the pubertal period in porcine testicular tissues (Ran et al 2015. It was further reported that miR-499 targets the QKI gene and represses its expression in immature porcine Sertoli cells (Zhang et al 2015). Therefore, we speculated that miR-499 might play regulatory roles in immature porcine Sertoli cell growth.…”

Section: Discussionmentioning

confidence: 73%

miR-499 promotes immature porcine Sertoli cell growth through the PI3K/AKT pathway by targeting the PTEN gene

Ran

Luo

et al. 2020

Reproduction

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Sertoli cells are indispensable for normal spermatogenesis, and increasing evidence has shown that miRNAs participate in the regulation of Sertoli cell growth. However, the functions and regulatory mechanisms of miRNAs in Sertoli cells of domestic animals have not been fully investigated. In the present study, we mainly investigated the regulatory roles of miR-499 in immature porcine Sertoli cells. The results showed that miR-499 was mainly located in the basement section of seminiferous tubules of prepubertal porcine testicular tissue. Overexpression of miR-499 promoted cell proliferation and inhibited apoptosis, whereas miR-499 inhibition resulted in the opposite effect. The PTEN gene was directly targeted by miR-499, and the expression of mRNA and protein was also negatively regulated by miR-499 in immature porcine Sertoli cells. siRNA-induced PTEN knockdown resulted in a similar effect as an overexpression of miR-499 and abolished the effects of miR-499 inhibition on immature porcine Sertoli cells. Moreover, both miR-499 overexpression and the PTEN knockdown activated the PI3K/AKT signaling pathway, whereas inhibition of the PI3K/AKT signaling pathway caused immature porcine Sertoli cell apoptosis and inhibited cell proliferation. Overall, miR-499 promotes proliferation and inhibits apoptosis in immature porcine Sertoli cells through the PI3K/AKT pathway by targeting the PTEN gene. This study provides novel insights into the effects of miR-499 in spermatogenesis through the regulation of immature Sertoli cell proliferation and apoptosis.

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Differential Expression of miR-499 and Validation of Predicted Target Genes in the Testicular Tissue of Swine at Different Developmental Stages

Cited by 9 publications

References 22 publications

Integrated analysis of miRNA and mRNA expression profiles in tilapia gonads at an early stage of sex differentiation

Integrated analysis of miRNA and mRNA expression profiles in tilapia gonads at an early stage of sex differentiation

Apoptosis of porcine Sertoli cells is inhibited by QKI-5 via regulating CASP8

miR-499 promotes immature porcine Sertoli cell growth through the PI3K/AKT pathway by targeting the PTEN gene

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