Differential expression of mast cell granules in samples of metastatic and non-metastatic colorectal cancer in patients

Rios, Paulina Alejandra Flores de los; Soto‐Domínguez, Adolfo; Vertti, Rubén Daniel Arellano-Pérez; Maldonado, Edmundo Erbey Castelán; Gauna, Sergio Everardo Velázquez; Martínez, Javier Morán; Salas-Treviño, Daniel; García-Garza, Rubén

doi:10.1016/j.acthis.2020.151618

Cited by 7 publications

(8 citation statements)

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“…Immunohistochemistry tests of tumour specimens collected from patients with colorectal cancer showed expression of chymase in the mast cells in the tumour microenvironment. It was also found that such expression was significantly higher in the group of subjects with distant metastases versus the group free of metastases [ 17 ]. Studies of HT-29 cell lines of colorectal cancer showed that these cells did not show ACE expression while showing AT1R and chymase expression.…”

Section: Discussionmentioning

confidence: 99%

Risk factors for long-term survival in patients with ypN+ M0 rectal cancer after radical anterior resection

et al. 2022

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Background Regional lymph node metastases are the main adverse prognostic factor in patients with rectal cancer without distant metastases. There are discrepancies, however, regarding additional risk factors in the group of ypN + M0 patients. The purpose of the study was to assess clinical and pathological factors affecting long-term oncological outcomes in the group of ypN + M0 patients after radical rectal anterior resection. Methods 112 patients with ypN + M0 rectal cancer after neoadjuvant therapy and radical anterior resection were subject to a retrospective analysis. The effect of potential factors on survival was assessed with the use of Kaplan–Meier curves together with a log-rank test and multiple factor Cox proportional hazards model. Results In the multiple factor Cox analysis, adverse factors affecting disease-free survival (DFS) were: the use of angiotensin-converting enzyme inhibitors (ACEIs) (hazard ratio HR: 3.11, 95% CI 1.01–9.56, p = 0.047), presence of perineural invasion (HR: 7.27, 95% CI 2.74–19.3, p < 0.001) and occurrence of postoperative complications (HR: 6.79, 95% CI 2.09–22.11, p = 0.001), while a positive factor was the negative lymph node (NLN) count > 7 (HR: 0.33, 95% CI 0.12–0.88, p = 0.026). In the disease-specific survival (DSS) analysis, an adverse factor was the use of ACEIs (HR: 4.275, 95% CI 1.44–12.694, p = 0.009), while a positive effect was caused by NLN > 5 (HR: 0.22, 95% CI 0.082–0.586, p = 0.002). Conclusions The use of ACEIs may have a negative effect on long-term treatment outcomes in patients with ypN + M0 rectal cancer. In this group of patients, the NLN count seems to be an important prognostic factor, as well.

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Section: Discussionmentioning

confidence: 99%

Risk factors for long-term survival in patients with ypN+ M0 rectal cancer after radical anterior resection

et al. 2022

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“…All samples were evaluated by 3 independent specialists in Morphology using previously published criteria. 34…”

Section: Immunohistochemical Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…In this study, a morphometric analysis was performed in the immunolabeling method by light microscopy, at a 40x magnification in 8 representative fields scattered in the preparations as follows. 34 The color parameters, hue distribution, saturation, and luminance were established in the capture software and were the same for all the images obtained. From the images obtained, the brown hue of the positivity to CLDN 2 was manually selected, and the program (with the detection threshold of the control sample pre-calibrated) converted the interval color to grayscale and the other tissue components to white.…”

Section: Morphometric Analysismentioning

confidence: 99%

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Antioxidant Effect of Jatropha dioica Extract on Immunoreactivity of Claudin 2 in the Kidney of Rats with Induced Diabetes

Ramírez-Moreno

García-Garza

Escobar

et al. 2023

Natural Product Communications

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Introduction Diabetes is related to different complications, such as nephropathy. In kidneys, the tight junctions (TJ) regulate the paracellular transport of solutes and water. The claudins are the most significant component in the TJ and are expressed throughout the epithelium and modulated permeability. Objective To evaluate the antioxidant effect of Jatropha dioica (JD) extract and claudin 2 (CLDN 2) in the kidney of rats with induced diabetes. Methods It is an experimental, explanatory, prospective, longitudinal, and analytical study. The effect of hydro-alcoholic extract from JD root on the presence of CLDN 2 in rats with induced diabetes was evaluated. The experiment lasting 21 days, treated 40 rats with and without induced diabetes by an intraperitoneal (IP) injection of alloxan. The study groups were exposed to JD extract at 25, 50, and 100 mg/kg. There was no significant morphological difference in the renal tissue morphology of the treated and control groups. Conclusion These results demonstrate that JD extract at 50 mg/kg ameliorated the tubular damage induced by diabetes, and the mechanism might be related to the CLDN2 expression. Finally, JD could also increase total antioxidant capacity to protect the kidney from oxidative stress.

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“…Previous tissue-based studies of intestinal MCs in human CRC either used no MC subtyping (histochemical stainings) [21,22] or IHC-based methodologies to subtype MCs regarding to their protease content as follows: tryptase (MC T ) [3,5,9,23]; chymase (MC C ); or tryptase and chymase (MC TC ) positive ( + ) MCs [7,8,24,25]. Indeed, some authors occasionally mention the occurrence of intestinal MCs in close proximity to the intestinal epithelium [22], but a clear discrimination between ieMMCs and stromal MCs in human CRC has not been carried out so far.…”

Section: Introductionmentioning

confidence: 99%

Comparative Study of the Role of Interepithelial Mucosal Mast Cells in the Context of Intestinal Adenoma-Carcinoma Progression

Groll

Silva

Sarker

et al. 2022

Cancers

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Mast cells (MCs) are crucial players in the relationship between the tumor microenvironment (TME) and cancer cells and have been shown to influence angiogenesis and progression of human colorectal cancer (CRC). However, the role of MCs in the TME is controversially discussed as either pro- or anti-tumorigenic. Genetically engineered mouse models (GEMMs) are the most frequently used in vivo models for human CRC research. In the murine intestine there are at least three different MC subtypes: interepithelial mucosal mast cells (ieMMCs), lamina proprial mucosal mast cells (lpMMCs) and connective tissue mast cells (CTMCs). Interepithelial mucosal mast cells (ieMMCs) in (pre-)neoplastic intestinal formalin-fixed paraffin-embedded (FFPE) specimens of mouse models (total lesions n = 274) and human patients (n = 104) were immunohistochemically identified and semiquantitatively scored. Scores were analyzed along the adenoma-carcinoma sequence in humans and 12 GEMMs of small and large intestinal cancer. The presence of ieMMCs was a common finding in intestinal adenomas and carcinomas in mice and humans. The number of ieMMCs decreased in the course of colonic adenoma-carcinoma sequence in both species (p < 0.001). However, this dynamic cellular state was not observed for small intestinal murine tumors. Furthermore, ieMMC scores were higher in GEMMs with altered Wnt signaling (active β-catenin) than in GEMMs with altered MAPK signaling and wildtypes (WT). In conclusion, we hypothesize that, besides stromal MCs (lpMMCs/CTMCs), particularly the ieMMC subset is important for onset and progression of intestinal neoplasia and may interact with the adjacent neoplastic epithelial cells in dependence on the molecular environment. Moreover, our study indicates the need for adequate GEMMs for the investigation of the intestinal immunologic TME.

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Differential expression of mast cell granules in samples of metastatic and non-metastatic colorectal cancer in patients

Cited by 7 publications

References 15 publications

Risk factors for long-term survival in patients with ypN+ M0 rectal cancer after radical anterior resection

Risk factors for long-term survival in patients with ypN+ M0 rectal cancer after radical anterior resection

Antioxidant Effect of Jatropha dioica Extract on Immunoreactivity of Claudin 2 in the Kidney of Rats with Induced Diabetes

Comparative Study of the Role of Interepithelial Mucosal Mast Cells in the Context of Intestinal Adenoma-Carcinoma Progression

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