Antioxidant Effect of Jatropha dioica Extract on Immunoreactivity of Claudin 2 in the Kidney of Rats with Induced Diabetes

Abstract

Introduction Diabetes is related to different complications, such as nephropathy. In kidneys, the tight junctions (TJ) regulate the paracellular transport of solutes and water. The claudins are the most significant component in the TJ and are expressed throughout the epithelium and modulated permeability. Objective To evaluate the antioxidant effect of Jatropha dioica (JD) extract and claudin 2 (CLDN 2) in the kidney of rats with induced diabetes. Methods It is an experimental, explanatory, prospective, longitudinal, and analytical study. The effect of hydro-alcoholic extract from JD root on the presence of CLDN 2 in rats with induced diabetes was evaluated. The experiment lasting 21 days, treated 40 rats with and without induced diabetes by an intraperitoneal (IP) injection of alloxan. The study groups were exposed to JD extract at 25, 50, and 100 mg/kg. There was no significant morphological difference in the renal tissue morphology of the treated and control groups. Conclusion These results demonstrate that JD extract at 50 mg/kg ameliorated the tubular damage induced by diabetes, and the mechanism might be related to the CLDN2 expression. Finally, JD could also increase total antioxidant capacity to protect the kidney from oxidative stress.