Differential Expression of High-Affinity Melatonin Receptors (MT1) in Normal and Malignant Human Breast Tissue

Dillon, Dionne; Easley, Samantha; Asch, Bonnie B.; Cheney, Richard; Brydon, Lena; Jockers, Ralf; Winston, Janet S.; Brooks, John S.; Hurd, Thelma C.; Asch, Harold L.

doi:10.1309/1t4v-ct1g-ubjp-3ehp

Cited by 67 publications

(67 citation statements)

References 25 publications

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“…7, B and C). Similar B max and EC 50 values were obtained for the MT 1 wild-type and the MUPP1 binding-deficient MT 1 -DSA mutant (Fig. 7, C and D).…”

Section: G I Coupling and High Affinity Agonist Binding To Mt 1 Depensupporting

confidence: 82%

“…In both cases, MUPP1 is removed far from its potential membrane partners, thus impeding any interaction with them. Regulation of the MT 1 /MUPP1 interaction is likely to occur under these circumstances, since functional MT 1 expression has been shown in keratinocytes (49) and mammary tumors (50). Recently, MUPP1 has been shown to be robustly up-regulated by hypertonicity and to be important in the osmotic stress response in tight junctions of kidney cells (51).…”

Section: Discussionmentioning

confidence: 99%

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The PDZ Protein Mupp1 Promotes Gi Coupling and Signaling of the Mt1 Melatonin Receptor

Guillaume

Daulat

Maurice

et al. 2008

Journal of Biological Chemistry

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The concept of organized networks has emerged in the field of cellular signaling in the last few years. Assembling the different partners in close proximity optimizes the spatial and temporal organization and the specificity of the cellular response. The assembly of these multimolecular complexes occurs through the interaction of modular domains recognizing their target counterparts. PDZ domains are widely spread modules exhibiting this function. Data bank exploration with SMART (1) identifies 584 PDZ domains in 328 different proteins in the human genome.G protein-coupled receptors (GPCRs) 5 constitute the largest family of membrane receptors, and many of the more than 750 members have been shown to interact with PDZ domain-containing proteins, either constitutively or upon agonist activation (2). Binding of PDZ proteins to GPCRs has been reported to primarily regulate subcellular localization, trafficking, and stability of receptors (3). For instance, binding of MUPP1 and syntrophins to the ␥-aminobutyric acid type B (GABA B ) receptor and the ␣ 1D -adrenergic receptor, respectively, significantly increases receptor stability (4, 5). In other cases, PDZ scaffolds determine the subcellular localization of GPCRs (6) and receptor endocytosis as shown for PSD-95 and the 5-HT 2A serotonin and ␤ 1 -adrenergic receptors (7,8). PDZ proteins, such as NHERF and hScrib, are also important for the recycling of receptors to the cell surface (9 -11).Binding of PDZ proteins to GPCRs also modulates receptor signaling by assembling proteins involved in signal transduction. NHERF family proteins are known to regulate the activity of the Na ϩ /H ϩ exchanger through association with NHERF-1 (12) and to form a ternary complex with phospholipase C␤3 and GPCRs, which enhances the signaling efficiency of the receptor-mediated activation of the phospholipase C␤/Ca 2ϩ pathway (13-15). Binding of GIPC (GAIP-interacting protein, COOH terminus) to the COOH terminus of the D3 dopamine and the ␤ 1 -adrenergic receptor (16) decreased G␣ i -mediated signaling of these receptors most likely through RGS19, which binds to GIPC (17). Further examples of PDZ scaffolds that regulate GPCR signaling include a ternary complex formation around the PDZ scaffold MAGI-3, which binds to the GPCR frizzled-4 and Ltap to regulate the JNK signaling cascade (18), as well as PDZ-domain-containing Rho guanine nucleotide exchange factors that interact with lysophosphatidic acid 1 and 2 receptors to activate RhoA (19).

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“…7, B and C). Similar B max and EC 50 values were obtained for the MT 1 wild-type and the MUPP1 binding-deficient MT 1 -DSA mutant (Fig. 7, C and D).…”

Section: G I Coupling and High Affinity Agonist Binding To Mt 1 Depensupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

The PDZ Protein Mupp1 Promotes Gi Coupling and Signaling of the Mt1 Melatonin Receptor

Guillaume

Daulat

Maurice

et al. 2008

Journal of Biological Chemistry

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“…These effects had been observed in tumor cell lines of breast (39), prostate (40) and colon carcinoma (41). Melanoma is an aggressive form of skin cancer associated with high mortality and limited response to therapy once extended beyond the skin (42)(43)(44).…”

Section: Introductionmentioning

confidence: 78%

“…For example, studies in the colon 38 carcinoma cell line identified both the nuclear receptor RZR/ROR (ROR·) and the cell membrane receptor MT2, but not MT1, as being responsible for the oncostatic effect of melatonin (41,52,68,69). In contrast, the MT1 receptor was responsible for the enhancement of the tumor suppressing effect of melatonin in MCF-7 breast cancer cells (39,53) and in S-91 murine melanoma cells (45). In uveal melanoma cell lines, melatonin and agonists for MT1 and MT2 inhibited melanoma growth, whereas the nuclear receptor was not involved in growth suppression (47).…”

Section: Discussionmentioning

confidence: 99%

Oncostatic effects of the indole melatonin and expression of its cytosolic and nuclear receptors in cultured human melanoma cell lines

Fischer

Żmijewski²,

Zbytek³

et al. 2006

Int J Oncol

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Abstract.Melatonin has been shown to have oncostatic effects on malignant melanoma in vitro and in vivo. We studied the growth suppressive effects of melatonin over a wide range of concentrations in four melanoma cell lines (SBCE2, WM-98, WM-164 and SKMEL-188) representative for different growth stages and phenotype. Melanoma cells were incubated with melatonin 10 -12 -10 -3 M, and proliferation and clonogenicity was assessed at 12 h and 14 days, respectively. We also determined the expression of cytosolic quinone oxidoreductases NQO1, NQO2 (known as MT3 receptor) and nuclear receptor ROR· by RT-PCR. Melatonin at pharmacological concentrations (10 -3 -10 -7 M) suppressed proliferation in all melanoma cell lines. In SKMEL-188 cells cultured in serum-free media, melatonin at low concentrations (10 -12 -10 -10 M) also slightly attenuated the proliferation. The effects of pharmacological doses of melatonin were confirmed in the clonogenic assay. Expression of NQO1 was detected in all cell lines, whereas NQO2 and nuclear receptor ROR· including its isoform ROR·4 were present only in SBCE2, WM-164 and WM-98. Thus, melatonin differentially suppressed proliferation in melanoma cell lines of different behaviour. The intensity of the oncostatic response to melatonin could be related to the cell-line specific pattern of melatonin cellular receptors and cytosolic binding protein expression.

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“…β‐catenin, a central mediator in the Wnt/β‐catenin signalling pathway, is expressed in many solid tumours 7, 8. Nuclear translocation of β‐catenin is considered a marker of pathway activation 9.…”

Section: Resultsmentioning

confidence: 99%

HMQ‐T‐F2 exert antitumour effects by upregulation of Axin in human cervical HeLa cells

Dai

Yang

Yin

et al. 2018

J Cellular Molecular Medi

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Looking for novel, effective and less toxic therapies for cervical cancer is of significant importance. In this study, we reported that HMQ‐T‐F2(F2) significantly inhibited cell proliferation and transplantable tumour growth. Mechanistically, HMQ‐T‐F2 inhibited HeLa cell growth through repressing the expression and nuclear translocation of β‐catenin, enhancing Axin expression, as well as downregulating the Wnt downstream targeted proteins. Knock‐down of a checkpoint β‐catenin by siRNA significantly attenuated HeLa cell proliferation. Furthermore, XAV939, an inhibitor of β‐catenin, was used to treat HeLa cells and the results demonstrated that HMQ‐T‐F2 inhibited proliferation and migration via the inhibition of the Wnt/β‐catenin pathway.

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Differential Expression of High-Affinity Melatonin Receptors (MT1) in Normal and Malignant Human Breast Tissue

Cited by 67 publications

References 25 publications

The PDZ Protein Mupp1 Promotes Gi Coupling and Signaling of the Mt1 Melatonin Receptor

The PDZ Protein Mupp1 Promotes Gi Coupling and Signaling of the Mt1 Melatonin Receptor

Oncostatic effects of the indole melatonin and expression of its cytosolic and nuclear receptors in cultured human melanoma cell lines

HMQ‐T‐F2 exert antitumour effects by upregulation of Axin in human cervical HeLa cells

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