Differential expression of four sweet potato peroxidase genes in response to abscisic acid and ethephon

Kim, Kee-Yeun; Kwon, Hye-Kyoung; Kwon, Suk‐Yoon; Lee, Haeng‐Soon; Hur, Yoonkang; Bang, Jae-Wook; Choi, Kyunghee; Kwak, Sang‐Soo

doi:10.1016/s0031-9422(00)00014-5

Cited by 24 publications

(14 citation statements)

References 17 publications

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“…Enhanced activity of particular POX isoforms is frequently associated with responses to wounding, pathogen infection and other stresses (Mäder et al, 1980;Grisebach, 1981;Christensen et al, 1998;Hiraga et al, 2000a,b). Differential activation of various isoforms has been seen in response to pathogens (Lebeda et al, 2001), ethylene and abscisic acid (Kim et al, 2000), cytokinins (Limam et al, 1998), and aphid feeding (Chaman et al, 2001), and diverse stimuli elicit differential accumulation of mRNAs for a wide range of POXs in rice (Hiraga et al, 2000). It is important to note that differences in POX isozyme activity were not detectable in the present study by the spectrophotometric assays of total POX activity.…”

Section: Discussionmentioning

confidence: 99%

Biochemical Responses of Chestnut Oak to a Galling Cynipid

Allison

Schultz

2005

J Chem Ecol

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Abstract-We characterized the distribution of nutritional and defensive biochemical traits in galls elicited on chestnut oak (Quercus prinus L.) by the gall wasp Andricus petiolicolus Basse (Cynipidae) in comparison with gypsy moth-wounded and unwounded leaves. Gall cortex and epidermis exhibited elevated soluble peroxidase (POX) and soluble invertase activities, and greater condensed tannin concentrations than did nutritive tissues or leaves. Nutritive tissue, on which the insect feeds, contained few polyphenols, and lower POX and invertase activities compared with other gall tissues and leaves. Elevated total POX activity arose from a complex pattern of enhanced and suppressed isoform activities in galls. Invertase enzyme activity decreased in all tissues over the course of the 7-d study, although gypsy moth wounding suppressed this decline slightly in ungalled leaves. Our results indicate that the distribution of biochemical defenses in this typical cynipid gall differs significantly from the leaf tissue from which it is formed and support a role for invertases in establishing the gall as a sink. A. petiolicolus larvae do not induce, and may suppress, plant defense responses in nutritive tissue, while enzymatic activity and phenolic accumulation are enhanced in gall tissues surrounding feeding sites. These patterns suggest that the gall is manipulated by the insect to enhance its food and protective value.

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Section: Discussionmentioning

confidence: 99%

Biochemical Responses of Chestnut Oak to a Galling Cynipid

Allison

Schultz

2005

J Chem Ecol

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“…To measure peroxidase activity, 100 mg of fresh tissue was collected weekly and stored at -70~ for analysis (Kim et al, 2000). The samples were ground with liquid nitrogen and 0.5 mL Tris buffer (50 mM, pH 7.5) that had been supplemented with 1% (w/v)insoluble polyvinyl-pyrrolidone (PVP).…”

Section: Peroxidase Activitymentioning

confidence: 99%

“…Its activity increases in response to a variety of physical, chemical, and biological stresses (Faivre-Rampant et al, 1998;Kim et al, 2000;Moon et al, 2005;Wi et al, 2006). This enzyme is also involved in the H202 detoxification mechanism, in lignification, and in general metabolism of plant phenolics (Lavid et al, 2006).…”

mentioning

confidence: 99%

Increased ethylene and decreased phenolic compounds stimulate somatic embryo regeneration in leaf thin section cultures ofDoritaenopsis hybrid

2006

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The effects of endogenous levels of ethylene and phenolic compounds on somatic embryogenesis, medium-browning, and peroxidase activity were evaluated in thin section cultures of Doritaenopsis. Cultures were maintained for 8 weeks with four different treatments: i) thick leaf segment culture, ii) thin leaf section culture, iii) thin leaf section culture with ventilation, or iv) thin leaf section culture after explants were first washed. Explants cultured in closed vessels produced a larger number of somatic embryos than those reared in the ventilated vessels. This enhanced formation confirmed the greater involvement of accumulated ethylene under non-ventilated conditions, because wound-induced tissues from thin leaf sections normally release high level of ethylene. When explants were washed in the liquid medium and inoculated on the same solid medium, somatic embryo production was 1.7 and 18.5 times higher than in the thin section cultures and thick segment cultures, respectively. Reducing the level of phenolics in explants at the initial stage of culturing apparently stimulated this embryo regeneration.

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“…In addition, ABA mediates changes in membrane stability and phospholipid composition, as well as in the modification of gene expression at the transcriptional level (Owen, 1988). Recent evidence suggests that ABA up regulates expression of the genes responsible for peroxidases in sweet potato (lpomoea batatas L.) (Kim et al, 2000).…”

Section: Role Of Hormones In Stressmentioning

confidence: 99%