Increased ethylene and decreased phenolic compounds stimulate somatic embryo regeneration in leaf thin section cultures ofDoritaenopsis hybrid

Park, Soyoung; Shin, Kong Sik; Paek, Kee Yoeup

doi:10.1007/bf03178812

Cited by 14 publications

(6 citation statements)

References 30 publications

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“…Therefore, the decreased efficiency of regeneration resulting from the lower survived explant rate following dark preconditioning suggests that treatment with AOPP is a simpler and more efficient method than dark preconditioning. Exudation of phenolic compounds and medium browning was observed in leaf cultures of Doritaenopsis (Park et al, 2006). In the present study, treatment with AOPP decreased the level of phenolic compounds in the upper portion of explants and medium browning for 4 weeks of culture (Figs.…”

Section: Discussionsupporting

confidence: 66%

“…Medium browning was measured using a modified protocol (Krishna et al, 2008;Park et al, 2006). Media (5.0 g) sampled from two culture vessels were homogenized in a mortar with 80% ethyl alcohol.…”

Section: Medium Browningmentioning

confidence: 99%

“…Cutting of explants also induces browning and death of the tissues owing to activation of phenylpropanoid metabolism and accumulation of phenol compounds in explants of most plant species (Park et al, 2006;Tang and Newton, 2004). In general, phenol compound secretion is related to in vitro survived explants and regeneration success (Arezki et al, 2001;FernandezLorenzo et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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L-2-aminooxy-3-phenylpropionic acid (AOPP) Controls Browning and Promotes Adventitious Bud Formation in Neofinetia falcata in vitro

Mitsukuri

Johkan

Yamasaki

et al. 2010

J. Japan. Soc. Hort. Sci.

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The effect of L-2-aminooxy-3-phenylpropionic acid (AOPP) on bud formation was investigated by determination of total phenolics in explants and medium browning in the micropropagation of the epiphytic orchid Neofinetia falcata (Thunb.) H.H. Hu. Explants, upper portion of plantlets, were cultured on a medium containing 0, 0.01, 0.1 or 1.0 mM AOPP. Browning was decreased from 70% to 3% by increasing AOPP concentration up to 1.0 mM. All surviving explants developed buds at 0, 0.01 and 0.1 mM AOPP. The highest mean number of buds (7.2) was produced on medium containing 0.01 mM AOPP. Multiple buds were formed on the explants 8 weeks after planting and multiple shoots had developed after 12 weeks. Total phenolics continuously increased in control explants up to 2.03 mg·gFW −1 after 4 weeks. In contrast, in explants treated with 0.01 mM AOPP, total phenolics ceased to increase for 4 weeks of culture and remained at 1.46 mg·gFW −1 , but thereafter increased to 2.24 mg·gFW −1 , the same level as the control, after 8 weeks. Medium browning continuously increased in the control, whereas medium containing 0.01 mM AOPP showed little browning after 4 weeks of culture and thereafter increased to the same degree as the control. The present results suggest that 0.01 mM AOPP is the most suitable concentration to control browning and bud formation in N. falcata.

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Section: Discussionsupporting

confidence: 66%

Section: Medium Browningmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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L-2-aminooxy-3-phenylpropionic acid (AOPP) Controls Browning and Promotes Adventitious Bud Formation in Neofinetia falcata in vitro

Mitsukuri

Johkan

Yamasaki

et al. 2010

J. Japan. Soc. Hort. Sci.

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“…But the number of yellowish SE reduced at the highest concentration of TDZ. Besides, an increase in the number of yellowish embryos has been reported due to the fact that TDZ elevates ethylene and phenolic compounds in explants (Park et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Refining a protocol for somatic embryogenesis and plant regeneration of Phalaenopsis amabilis cv. Jinan from mature tissues

Ghahremani¹,

Daylami²,

Mirmasoumi³

et al. 2021

Turk J Agric For

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Phalaenopsis is one of the most remarkable commercial genera in Orchidaceae family.Using immature explants for tissue culture of orchid results in undesired characteristics such as genetic variation and long-lasting juvenility. In this study, we optimized a protocol for somatic embryogenesis of Phalaenopsis amabilis cv. Jinan, by using mature thin cell layer (TCL) explants to minimize immature explant limitations. We examined somatic embryogenesis from two types of mature explants, viz. leaf lTCL and flower stalk nodes tTCL at various concentrations of TDZ (0, 1, 2 and 3 mg L -1 ). The results demonstrated that lTCL explants cultured on medium supplemented with 3 mg L -1 TDZ produced the highest number of somatic embryos (SE) (21.5 embryos per explant), the lowest yellowish SE (7%), the highest plantlet regeneration (20.5 plantlets per explant) and the highest survival rate (94%) in comparison with tTCL explants. This protocol can be used in commercial mass propagation of Phalaenopsis to overcome the limitations such as long-term juvenility and genetic variation of plantlets regenerated in vitro by using immature explants.

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“…Bud formation using divided floret explants is thought to be suitable for multiplication of elite clones of H. radiata. Multiplication efficiency might be increased by the use of thin-section culture that has been reported in Doritaenopsis (Park et al, 2006), Dendrobium (Zhao et al, 2007), and Phalaenopsis (Sinha et al, 2007). Callus, which sometimes results in an increased incidence of somaclonal variation (Evans and Sharp, 1983), was not induced in as part of this regeneration procedure of our experiment; hence, the occurrence of somaclonal variation is likely to be very low.…”

Section: Discussionmentioning

confidence: 99%

Effects of Type of Explant and Dark Preconditioning on Bud Formation in Habenaria radiata (Thunb.) in Vitro

Mitsukuri¹,

Arita²,

Johkan³

et al. 2009

horts

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Habenaria radiata is a terrestrial orchid with beautiful bird-shaped petals. The wild H. radiata population has been severely affected by environmental disruption and overexploitation. In micropropagation of H. radiata, although aseptic germination has been studied, tissue culture methods have not yet been established. Shoot apexes and leaf explants from vegetative plants and flower stalks, stolons, and floret explants from reproductive plants were chosen for this study. Explants were cultured on half-strength inorganic salts and full-strength vitamins of Murashige and Skoog (1/2 MS) medium containing 30 g·L−1 sucrose, 8 g·L−1 agar (pH 5.6) supplemented with 4.44 μM N6-benzyladenine, and 0.54 μM α-naphthaleneacetic acid. After 8 weeks of culture, the highest survival rate was obtained with floret explants excised from plants at the reproductive phase. In floret culture, the number of adventitious bud formation per explant was 5.4 per upper floret and 4.0 per lower floret. Dark preconditioning, which inhibited browning and contamination, of explants before shoot apex culture increased survival rates of explants (53%) and bud formation (83%). Consequently, a tissue culture method using florets and shoot apexes as explant material was established for H. radiata.

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Increased ethylene and decreased phenolic compounds stimulate somatic embryo regeneration in leaf thin section cultures ofDoritaenopsis hybrid

Cited by 14 publications

References 30 publications

L-2-aminooxy-3-phenylpropionic acid (AOPP) Controls Browning and Promotes Adventitious Bud Formation in Neofinetia falcata in vitro

L-2-aminooxy-3-phenylpropionic acid (AOPP) Controls Browning and Promotes Adventitious Bud Formation in Neofinetia falcata in vitro

Refining a protocol for somatic embryogenesis and plant regeneration of Phalaenopsis amabilis cv. Jinan from mature tissues

Effects of Type of Explant and Dark Preconditioning on Bud Formation in Habenaria radiata (Thunb.) in Vitro

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