Differential expression of extracellular matrix metalloproteinase inducer (EMMPRIN/CD147) in avian tibial dyschondroplasia

Abstract

Tibial dyschondroplasia (TD) is an avian bone disorder of different aetiologies that may be associated with lameness. The disorder is characterized by focal disruption of endochondral bone formation, with a lack of matrix proteolysis and an accumulation of non-mineralized avascular cartilage. The aim of this study was to determine the expression of extracellular matrix metalloproteinase inducer (EMMPRIN/CD147) in normal, thiram-induced TD lesions and in the process of recovery from TD in broiler chickens. An extracellular matrix (ECM) degrading enzyme, matrix metalloproteinase-9 (MMP-9), was selected to investigate the effects of CD147 in the degradation of ECM. Gene expression was analysed by quantitative real-time polymerase chain reaction and protein levels by immunohistochemistry and western blotting. The birds were divided into three groups: thiram fed; recovery; and controls. Genes encoding CD147 and MMP-9 were down-regulated during the development of the disease, and were up-regulated during recovery. Western blotting also showed lower protein levels of CD147 in TD, which increased during the recovery phase associated with ECM degradation and growth plate repair. The findings of this study suggest that ECM has a crucial role in the occurrence of TD and that CD147 appears to play a pivotal role in matrix proteolysis in the chicken, similar to that in other species.

Tetramethylpyrazine Mitigates Toxicity and Liver Oxidative Stress in Tibial Dyschondroplasia Chickens

“…After that on day 4 post hatch, chickens were distributed in 2 groups; Control (n=60) and Thiram (n=120) group. Tibial dyschondroplasia was induced by adding thiram @ 50 mg per kg of feed to thiram group ( Shahzad et al, 2014a;Shahzad et al, 2015;Iqbal et al, 2016) while control group was offered normal diet. On day 7 post hatch, after induction of TD, thiram group was equally divided into two groups designated as TD group (n=60) and TMP treatment group (n=60).…”

“…However, most recent molecular study showed that the occurrence of TD is associated with inhibition of angiogenesis and genes related to bone vascularization and mineralization (Shahzad et al, 2015;Nabi et al, 2016).…”

Earlier hatching time predisposes Cobb broiler chickens to tibial dyschondroplasia

“…TD is described as one of the most prevalent leg weakness conditions seen in modern broilers (Yalçin et al, 2007), and lesions have been found to be present in 30% of broiler flock (Crespo and Shivaprasad, 2011;Shahzad et al, 2015). Accurate prevalence figures are not readily available as TD is mostly sub-clinical and does not result in huge culling rates (Crespo and Shivaprasad, 2011) and has a very variable incidence (Leach and Monsonego-Ornan, 2007), but it does contribute to general leg weakness expressed as reluctance to move.…”

Salvia Miltiorrhiza Reinstates Growth Plate Width, Reduces Liver Oxidative Stress and Toxicity in Avian Tibial Dyschondroplasia

“…TD is characterized as avascularized and non-mineralized cartilage in the growth plate (Tian et al, 2013). The etiology of TD is associated with several factors, however, most recent molecular studies showed that the occurrence of TD is associated with inhibition of angiogenesis, related genetic factors and genes contribute to bone vascularization and mineralization (Nabi et al, 2016a;Shahzad et al, 2015).Thiram (tetramethyl thiuram disulfide) is generally used as apesticide and fungicide in agriculture sector. Experimental addition of thiram to poultry feed induces TD.…”

“…Thiram interferes with normal growth plate metabolism and development of bone that causes TD in chicken (Rath et al, 2005;Nabi et al, 2016a;Shahzad et al, 2015).…”