Differential expression of Cyclin D1 in keratin-producing odontogenic cysts

Abstract

Objetives: The aim of the present study was to analyze the expression levels of Cyclin D1 (CCD1), a nuclear protein that plays a crucial role in cell cycle progression, in a series of keratin-producing odontogenic cysts. Study Design: A total of 58 keratin-producing odontogenic cysts, diagnosed over ten years and classified according to the WHO 2005 criteria, were immunohistochemically analyzed in terms of CCD1 expression, which was quantified in the basal, suprabasal and intermediate/superficial epithelial compartments. The extent of immunostaining was measured as a proportion of total epithelial thickness. Quantified immunohistochemical data were correlated with clinicopathological features and clinical recurrence. Results: Keratin-producing odontogenic cysts were classified as 6 syndromic keratocystic odontogenic tumors (S-KCOT), 40 sporadic or non-syndromic KCOT (NS-KCOT) and 12 orthokeratinized odontogenic cysts (OOC). Immunohistochemically, CCD1 staining was evident predominantly in the parabasal region of all cystic lesions, but among-lesion differences were apparent, showing a clear expansion of parabasal compartment especially in the S-KCOT, followed to a lesser extent in the NS-KCOT, and being much more reduced in the OOC, which had the greatest average epithelial thickness. Conclusions: The differential expression of CCD1 noted in the present study suggests that dysregulation of cell cycle progression from G1 to the S phase contributes to the different aggressiveness of these lesions. However, CCD1 expression levels did not predict NS-KCOT recurrence, which is likely influenced by factors unrelated to lesion biology. Key words:Keratin-producing odontogenic cyst, keratocyst, keratocystic odontogenic tumor, nevoid basal cell carcinoma syndrome, orthokeratinized odontogenic cyst, cyclin D1, immunohistochemistry.

Survivin, cyclin D1, and p21hras in keratocystic odontogenic tumors before and after decompression

“…This result is consistent with previous studies (Lo Muzio et al, 1999;Kimi et al, 2001;De Vicente et al, 2010;Vera-Sirera et al, 2015). An initial report on cyclin D1 in KCOTs by Lo Muzio and co-workers reported that its expression was typical for syndromic KCOTs while sporadic lesions were negative (Lo Muzio et al, 1999).…”

“…An initial report on cyclin D1 in KCOTs by Lo Muzio and co-workers reported that its expression was typical for syndromic KCOTs while sporadic lesions were negative (Lo Muzio et al, 1999). Further studies demonstrated that cyclin D1 immunostaining is an inherent feature of these tumors, whether they are primary, recurrent, or syndromic (Kimi et al, 2001;De Vicente et al, 2010;Vera-Sirera et al, 2015). Despite the fact that cyclin D1 immunoreactivity was noted in samples of normal oral mucosa (Kimi et al, 2001;Gurgel et al, 2014), our study showed that all samples of pericoronal follicles remained negative for cyclin D1.…”

Immunohistochemical expression of glucose transporter 1 in keratin-producing odontogenic cysts

“…In the present study, we have analysed for the first time to our knowledge, the expression of GLUT1, a protein transporter involved in the active uptake of glucose across cell membranes, in a series of KPOCs, which constitute a heterogeneous group of cystic odontogenic lesions, that are often aggressive in character, with high rates of recurrence and multifocality [ 1 ], and are associated with a marked proliferative activity [ 2 , 3 ].…”

“…Keratin-producing odontogenic cysts (KPOCs) form a heterogeneous group of cystic lesions that are often aggressive in character, with high rates of recurrence and multifocality [ 1 ], associated to a marked proliferative activity [ 2 , 3 ]. The lesional spectrum of KPOCs includes primarily orthokeratinised odontogenic cysts (OOC) and odontogenic keratocysts, which, according to WHO guidelines [ 4 ] are also referred to as keratocystic odontogenic tumours (KCOTs), in accordance with KCOTs being true tumoural growths.…”

Glucose Transporter 1 Expression in Odontogenic Keratocyst, Dentigerous Cyst, and Ameloblastoma: An Immunohistochemical Study

“…[17][18][19][20] The high infiltrative growth of OKC was comparable to AM. Due to this nature of OKC, a change in its terminology was long proposed and suggestions were made to consider it to be a neoplasm instead of a cyst.…”