Immunohistochemical expression of glucose transporter 1 in keratin-producing odontogenic cysts

Abstract

BackgroundKeratin-producing odontogenic cysts (KPOCs) are a group of cystic lesions that are often aggressive, with high rates of recurrence and multifocality. KPOCs included orthokeratinised odontogenic cyst (OOC) and parakeratotic odontogenic cysts, which are now considered true tumours denominated keratocystic odontogenic tumours (KCOTs). GLUT1 is a protein transporter that is involved in the active uptake of glucose across cell membranes and that is overexpressed in tumours in close correlation with the proliferation rate and positron emission tomography (PET) imaging results.MethodsA series of 58 keratin-producing odontogenic cysts was evaluated histologically and immunohistochemically in terms of GLUT1 expression. Different data were correlated using the beta regression model in relation to histological type and immunohistochemical expression of GLUT1, which was quantified using two different morphological methods.ResultsKPOC cases comprised 12 OOCs and 46 KCOTs, the latter corresponding to 6 syndromic and 40 sporadic KCOTs. GLUT1 expression was very low in OOC cases compared with KCOT cases, with statistical significant differences when quantification was considered. Different GLUT1 localisation patterns were revealed by immunostaining, with the parabasal cells showing higher reactivity in KCOTs. However, among KCOTs cases, GLUT1 expression was unable to establish differences between syndromic and sporadic cases.ConclusionsGLUT1 expression differentiated between OOC and KCOT cases, with significantly higher expression in KCOTs, but did not differentiate between syndromic and sporadic KCOT cases. However, given the structural characteristics of KCOTs, we hypothesised that PET imaging methodology is probably not a useful diagnostic tool for KCOTs. Further studies of GLUT1 expression and PET examination in KCOT series are needed to confirm this last hypothesis.

Glucose Transporter 1 Expression in Odontogenic Keratocyst, Dentigerous Cyst, and Ameloblastoma: An Immunohistochemical Study

“…29 In the present study, when the GLUT-1 expression was evaluated in OKC, it showed more number of labeled cells, strong intensity of staining, and both membrane and cytoplasmic staining. Similar findings were also reported by Vasconcelos et al 15 and Vera-Sirera et al 16 When the number of labeled cells was considered, in contrast to OKC, AM and DC showed less number of labeled cells. However, Vasconcelos et al 15 found all three lesions expressing more number of labeled cells.…”

“…However, Vasconcelos et al 15 found all three lesions expressing more number of labeled cells. Vera-Sirera et al 16 compared the GLUT-1 expression of OKC with orthokeratinized odontogenic cyst and found higher expression in OKC. They proposed that the higher expression of GLUT-1 in OKC can be attributed to the high cellular proliferation as seen in various other types of tumors.…”

“…14 Very few studies are present in the literature enumerating the GLUT-1 expression in odontogenic cysts, such as OKC and DC. 15,16 The aim of our study was to evaluate GLUT-1 expression in AM, OKC, and DC by immunohistochemistry to understand the metabolic behavior of each. This may throw some light on their biology.…”

Orthokeratinized odontogenic cysts: A clinicopathologic study of 159 cases and molecular evidence for the absence of PTCH1 mutations

“…After reviewing the reported OOCs in English literature, we found that more than 300 cases have been reported in different populations since 1981 (Table S1). 1–4,6,16–21 Thirty‐eight cases in present study overlapped with our previous reports 6,22 . After removing duplicated data, a total of 280 reported cases were analyzed as follows.…”

Glucose transporter 1 expression in ameloblastoma and odontogenic keratocyst – A comparative immunohistochemical study

“…study which states that high intensity and pronounced membrane staining of GLUT1 in parabasal cells of OKCs. [ 13 ] Previous studies on OKC using proliferative markers such as Ki67, PCNA and AgNOR and cell cycle regulatory factor cyclin D1 showed increased expression in the supra basal layers of OKC when compared to basal and superficial layers. [ 14 15 16 ] This suggests that the cells in the supra basal cells are in the proliferative pool which reinforces the fact that OKC epithelial lining presents a different pattern of cell proliferation.…”