Differential expression and localization of de-novo synthesized endometriotic haptoglobin in endometrium and endometriotic lesions

Sharpe-Timms, Kathy L.; Ricke, Emily A.; Piva, Marta; Horowitz, Gary M.

doi:10.1093/humrep/15.10.2180

Cited by 57 publications

(46 citation statements)

References 35 publications

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“…The H-SCORE was calculated with the following equation: H-SCORE = Σ Pi (i + 1). Intensity (i) of HOXA10 nuclear staining is indicated by a value of 1, 2, or 3 (weak, moderate, or strong respectively), and Pi is the percentage of stained nuclei for each intensity, ranging from 0 -100% (46,47). The three H-SCORE results for the glands from each slide were averaged, and the three H-SCORE results for the stroma were averaged.…”

Section: Methodsmentioning

confidence: 99%

GnRH antagonists may affect endometrial receptivity

Rackow

Kliman

Taylor

2008

Fertility and Sterility

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Study objective-HOXA10 is an essential regulator of endometrial receptivity. To determine the effect of gonadotropin releasing hormone (GnRH) antagonists on endometrial receptivity we assessed endometrial HOXA10 expression in GnRH antagonist, GnRH agonist, and natural cycles. Design-Prospective case-control study Setting-University academic medical centerPatients-Nineteen subjects were included: 12 subjects underwent controlled ovarian hyperstimulation (COH) with recombinant follicle stimulating hormone (rFSH) and used either a GnRH antagonist or a GnRH agonist; 7 control subjects underwent natural cycles.Interventions-Pipelle endometrial biopsies were obtained 11 days after human chorionic gonadotropin (hCG) administration or spontaneous luteinizing hormone (LH) surge in untreated cycles, respectively. Immunohistochemistry was used to assess HOXA10 protein expression in endometrial glands and stroma. Main outcome measure(s)-Endometrial HOXA10 protein expressionResults-HOXA10 expression was significantly decreased in endometrial stromal cells in GnRH antagonist treated cycles compared with GnRH agonist treated cycles or natural cycle controls. There was no significant difference in glandular cell HOXA10 expression among the three groups.Conclusions-Use of GnRH antagonists may be associated with impaired HOXA10 expression in endometrial stromal cells, and thus may affect endometrial receptivity.

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Section: Methodsmentioning

confidence: 99%

GnRH antagonists may affect endometrial receptivity

Rackow

Kliman

Taylor

2008

Fertility and Sterility

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“…The H-SCORE was calculated with the following equation: H-SCORE = Σ Pi (i + 1). Intensity of HOXA10 nuclear staining is indicated by a value of 0, 1, 2, or 3 (none, weak, moderate, or strong respectively), and Pi is the percentage of stained nuclei for each intensity, ranging from 0 -100% (49,50). For each slide, the two H-SCORE results for glandular cells were averaged; the same process was employed for stromal cells.…”

Section: Immunohistochemistrymentioning

confidence: 99%

Submucosal uterine leiomyomas have a global effect on molecular determinants of endometrial receptivity

Rackow

Taylor

2010

Fertility and Sterility

190

143

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Study objective-To evaluate the effect of uterine leiomyomas on the endometrium using molecular markers of endometrial receptivity: HOXA10, HOXA11, LIF, and BTEB1. Design-Case-control study Setting-University medical centerPatients-Thirty reproductive-age women with submucosal, intramural, or no uterine myomas who underwent hysteroscopy or hysterectomy.Interventions-Proliferative phase endometrial sampling was performed at the time of surgery. In uteri with a submucosal myoma, directed endometrial biopsies were obtained over the myoma and over normal myometrium.Main outcome measures-Endometrial HOXA10 expression was evaluated as a primary end point using quantitative real time RT-PCR and immunohistochemistry. HOXA11, BTEB1, and LIF were evaluated using real time RT-PCR.Results-Endometrial HOXA10 and HOXA11 mRNA expression were significantly decreased in uteri with submucosal myomas compared to controls and to uteri with intramural myomas. A similar trend was seen in BTEB1 mRNA expression, however no difference was found in LIF mRNA expression. Immunohistochemistry localized the decrease in endometrial HOXA10 protein expression to stroma. In the presence of a submucosal myoma, there were no regional differences in gene expression.Conclusions-The molecular mechanism by which submucosal myomas adversely affect reproduction includes a global decrease in endometrial HOX gene expression, not simply a focal change over the myoma. This may explain the reproductive dysfunction observed with submucosal myomas.

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“…The H-score was calculated using the following equation: H-score = Σ Pi (i+1). Intensity of HOXA10 nuclear staining is indicated by a value of 0, 1, 2, or 3 (none, weak, moderate, strong), and Pi is the percentage of stained nuclei for each intensity (33,34).…”

mentioning

confidence: 99%