Differential effects of TGF-β1 and FGF-2 on SDF-1α expression in human periodontal ligament cells derived from deciduous teeth in vitro

Hasegawa, Tomokazu; Chosa, Naoyuki; Asakawa, Takeyoshi; Yoshimura, Yoshitaka; Fujihara, Yuri; Kitamura, Takamasa; Tanaka, Mitsuro; Ishisaki, Akira; Mitome, Masato

doi:10.3892/ijmm.2012.957

Cited by 9 publications

(9 citation statements)

References 25 publications

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“…Indeed, the expression level was much higher than that in human dermal fibroblasts (17). Hasegawa et al reported that SDF-1α expression was increased in hPDLF by treatment with transforming growth factor-β1 but inhibited by treatment with basic fibroblast growth factor-2 (18), which suggests that fibroblasts exhibit cell type-specific reactivity to external stimuli. ANG, VEGF, and SDF-1α are potent angiogenic factors.…”

Section: Discussionmentioning

confidence: 98%

Hypoxia-induced upregulation of angiogenic factors in immortalized human periodontal ligament fibroblasts

et al. 2018

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Hypoxia induces complex cellular responses that are mediated by a key transcription factor, hypoxia-inducible factor-1 (HIF-1). HIF-1 promotes production of cytokines and angiogenic factors and contributes to recovery of injured tissues. In the present study, expressions of angiogenin (ANG) and vascular endothelial growth factor (VEGF), which are potent angiogenic factors in mammalian tissues, were examined in immortalized fibroblasts exposed to hypoxia. After 24 h of exposure to hypoxia, ANG and VEGF mRNAs expressions were significantly elevated in periodontal ligament (PDL) fibroblasts but not in embryonic fibroblasts. Hypoxia also increased productions of ANG and VEGF proteins in PDL fibroblasts. HIF-1α mRNA expression was not affected by hypoxia in either fibroblast, although HIF-1α protein expression was enhanced after exposure to hypoxia. Treatment of PDL fibroblasts with dimethyloxaloylglycine, a prolyl hydroxylase inhibitor that stabilizes the HIF-1α protein, significantly increased expressions of ANG and VEGF mRNAs under normoxia. This suggests that stabilization of HIF-1α is crucial for upregulation of ANG and VEGF in PDL fibroblasts. These results indicate that, under hypoxic conditions, HIF-1α upregulates synthesis of ANG and VEGF in PDL fibroblasts and promotes angiogenesis.

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Section: Discussionmentioning

confidence: 98%

Hypoxia-induced upregulation of angiogenic factors in immortalized human periodontal ligament fibroblasts

et al. 2018

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“…It has been demonstrated in previous studies that a number of cytokines and bioactive materials have clinical uses (32,33). One such cytokine molecule, FGF-2, is a multifunctional growth factor that exerts various effects, including the induction of proliferation and differentiation of a wide range of mesodermal and neuroectodermal cells (10,32,33). FGF-2 is also a crucial factor in wound healing and is involved in the induction of angiogenesis, cell proliferation and the accumulation and modulation of the extracellular matrix (34).…”

Section: Discussionmentioning

confidence: 99%

“…Transmembrane migration assay. Cell migration was evaluated using a Transwell system as previously described (10). Chambers with a 8-µm pore size and 6.4 mm in diameter were used (Corning Cell Culture Insert 24-well System; Sigma-Aldrich, St. Louis, MO, USA).…”

Section: Reverse Transcription Polymerase Chain Reaction (Rt-pcr)mentioning

confidence: 99%

Recruitment of mesenchymal stem cells by stromal cell-derived factor 1α in pulp cells from deciduous teeth

Akazawa

Hasegawa

Yoshimura

et al. 2015

International Journal of Molecular Medicine

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Dental pulp cells (DPCs), including dental pulp (DP) stem cells, play a role in dentine repair under certain conditions caused by bacterial infections associated with caries, tooth fracture and injury. Mesenchymal stem cells (MSCs) have also been shown to be involved in this process of repair. However, the mechanisms through which MSCs are recruited to the DP have not yet been elucidated. Therefore, the aim of the present in vitro study was to investigate whether stromal cell-derived factor 1α (SDF1)-C-X-C chemokine receptor type 4 (CXCR4) signaling is involved in tissue repair in the DP of deciduous teeth. A single-cell clone from DPCs (SDP11) and UE7T-13 cells were used as pulp cells and MSCs, respectively. The MG-63 and HuO9 cells, two osteosarcoma cell lines, were used as positive control cells. Reverse transcription polymerase chain reaction (RT-PCR) revealed that all cell lines (SDP11, UE7T-13 MG-63 and HuO9) were positive for both SDF1 and CXCR4 mRNA expression. Moreover, immunocytochemical analysis indicated that SDF1 and CXCR4 proteins were expressed in the SDP11 and UE7T-13 cells. SDF1 was also detected in the cell lysates (CLs) and conditioned medium (CM) collected from the SDP11 and UE7T-13 cells, and AMD3100, a specific antagonist of CXCR4, inhibited the migration of the UE7T-13 cells; this migration was induced by treatment with CM, which was collected from the SDP11 cells. In addition, real-time PCR showed that the expression of SDF1 in the SDP11 cells was inhibited by treatment with 20 ng/ml fibroblast growth factor (FGF)-2, and exposure to AZD4547, an inhibitor of the FGF receptor, blocked this inhibition. Collectively, these data suggest that SDF1 produced by DP plays an important role in homeostasis, repair and regeneration via the recruitment of MSCs.

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“…RNA (1 µg) was reversetranscribed to first-strand cDNA using a PrimeScript™ RT reagent kit (Takara Bio, Inc., Shiga, Japan) according to the manufacturer's instructions. A Thermal Cycler Dice real-time system (Takara Bio, Inc.) was used for real-time PCR (18)(19)(20). The cDNAs were amplified with SYBR ® Premix Ex Taq and specific primer pairs for fibroblast growth factor (FGF)-1 (21), FGF-2 (22), epidermal growth factor (EGF) (23), vascular endothelial growth factor (VEGF) (24,25), insulin-like growth factor-1 (IGF-1) (26), nerve growth factor (NGF), neurotrophin-3 (NT-3), brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF), neuropeptide Y, β-tubulin Ⅲ, 2' ,3'-cyclic-nucleotide phosphodiesterase (CNPase), microtubule-associated protein 2 (Map2), glial fibrillary acidic protein (GFAP), nestin, glutamate receptor 1 (GluR1) (27), glutamate receptor 2 (GluR2) (27), glutamate receptor 3 (GluR3) (27), glutamate receptor 4 (GluR4) (27), N-methyl D-aspartate receptor subtype 2B (NR2B) (28) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH).…”

Section: Volume Measurement Of the Granular Cell Layer Of The Dgmentioning

confidence: 99%

Forced mastication increases survival of adult neural stem cells in the hippocampal dentate gyrus

Akazawa

Kitamura

Fujihara

et al. 2012

International Journal of Molecular Medicine

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Abstract. In this study, we examined the effect of forced mastication on neurogenesis in the hippocampal dentate gyrus (DG) of adult mice. Six-week-old mice were subjected to either a hard or normal diet for 13 weeks. They received a daily injection of bromodeoxyuridine (BrdU) for 12 consecutive days beginning at 14 weeks of age. The number of BrdU-positive cells in the DG was counted 1 day after and 5 weeks after the final BrdU injection. The number of BrdU-positive cells 1 day after injection did not differ between the 2 diet groups. However, the number of BrdU-positive cells in the group fed the hard diet was significantly increased 5 weeks after BrdU injection compared to the group fed the normal diet. The results of the Morris water maze test showed that mice fed a hard diet required significantly less time to reach the platform than the control mice when tested at 10 days. Moreover, mice in the group fed the hard diet spent significantly more time in the former platform area than the group fed the normal diet, indicating that hard diet feeding improved spatial memory compared to normal diet feeding. Real-time PCR analysis showed that the expression of glutamate receptor 1 mRNA was significantly increased in the group fed the hard diet compared with the group fed the normal diet. These results suggest that mastication increases the survival of adult neural stem cells in the hippocampal DG.

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Differential effects of TGF-β1 and FGF-2 on SDF-1α expression in human periodontal ligament cells derived from deciduous teeth in vitro

Cited by 9 publications

References 25 publications

Hypoxia-induced upregulation of angiogenic factors in immortalized human periodontal ligament fibroblasts

Hypoxia-induced upregulation of angiogenic factors in immortalized human periodontal ligament fibroblasts

Recruitment of mesenchymal stem cells by stromal cell-derived factor 1α in pulp cells from deciduous teeth

Forced mastication increases survival of adult neural stem cells in the hippocampal dentate gyrus

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