Recruitment of mesenchymal stem cells by stromal cell-derived factor 1α in pulp cells from deciduous teeth

Abstract

Dental pulp cells (DPCs), including dental pulp (DP) stem cells, play a role in dentine repair under certain conditions caused by bacterial infections associated with caries, tooth fracture and injury. Mesenchymal stem cells (MSCs) have also been shown to be involved in this process of repair. However, the mechanisms through which MSCs are recruited to the DP have not yet been elucidated. Therefore, the aim of the present in vitro study was to investigate whether stromal cell-derived factor 1α (SDF1)-C-X-C chemokine receptor type 4 (CXCR4) signaling is involved in tissue repair in the DP of deciduous teeth. A single-cell clone from DPCs (SDP11) and UE7T-13 cells were used as pulp cells and MSCs, respectively. The MG-63 and HuO9 cells, two osteosarcoma cell lines, were used as positive control cells. Reverse transcription polymerase chain reaction (RT-PCR) revealed that all cell lines (SDP11, UE7T-13 MG-63 and HuO9) were positive for both SDF1 and CXCR4 mRNA expression. Moreover, immunocytochemical analysis indicated that SDF1 and CXCR4 proteins were expressed in the SDP11 and UE7T-13 cells. SDF1 was also detected in the cell lysates (CLs) and conditioned medium (CM) collected from the SDP11 and UE7T-13 cells, and AMD3100, a specific antagonist of CXCR4, inhibited the migration of the UE7T-13 cells; this migration was induced by treatment with CM, which was collected from the SDP11 cells. In addition, real-time PCR showed that the expression of SDF1 in the SDP11 cells was inhibited by treatment with 20 ng/ml fibroblast growth factor (FGF)-2, and exposure to AZD4547, an inhibitor of the FGF receptor, blocked this inhibition. Collectively, these data suggest that SDF1 produced by DP plays an important role in homeostasis, repair and regeneration via the recruitment of MSCs.

Pannexin 3 regulates proliferation and differentiation of odontoblasts via its hemichannel activities

“…The expression of p21, but not of p27, was significantly reduced by the knockdown of endogenous Panx3 (Fig 4D). Further, we have used single clone cell derived from deciduous tooth pulp cells, SDP11 cells [29]. SDP11 cells were cultured with BMP2 and Panx3 inhibitory peptide.…”

Stimulation of oral fibroblast chemokine receptors identifies CCR3 and CCR4 as potential wound healing targets

“…Although the appropriate receptors for CXCL8 and CXCL12 are expressed on gingiva fibroblasts, these chemokines did not affect proliferation, migration, or the secretion of wound healing mediators. Both CXCL8 and CXCL12 have been shown to influence skin fibroblast migration and CXCL12 is even thought to be a major factor in stem cell homing to sites of injury (Akazawa et al, ; Stuermer et al, ). For CXCL8, our results may possibly be explained by the fact that gingiva fibroblasts secrete large quantities of endogenous CXCL8 which would mask any effect of the recombinant CXCL8 used in this study (Buskermolen et al, ; Kosten et al, ).…”

Piezo type mechanosensitive ion channel component 1 functions as a regulator of the cell fate determination of mesenchymal stem cells

“…The UE7T-13 cells, a human bone marrow-derived MSC line infected with retroviruses expressing papillomavirus E7 and hTERT, were purchased from the Health Science Research Resources Bank (JCRB1154; Japan Health Sciences Foundation, Tokyo, Japan). The SDP11 cells, a human dental pulp-derived MSC line transfected with the pBABE-neo-hTERT plasmid, were recently established from the crown and root of healthy human deciduous teeth 58 , 59 . The primary human MSCs originated from a single human bone marrow (BMSCs) were obtained from Lonza (PT-2501).…”