Differential effects of high-carbohydrate and high-fat diets on hepatic lipogenesis in rats

Ferramosca, Alessandra; Conte, Annalea; Damiano, Fabrizio; Siculella, Luisa; Zara, Vincenzo

doi:10.1007/s00394-013-0613-8

Cited by 45 publications

(37 citation statements)

References 38 publications

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“…For our model application with focus on the FA content of a diet, we assumed that the carbohydrate content in the diet is in a normal, healthy range and, thus, does not contribute to an increased DNL. In support of this procedure is the fact that at the beginning of a high-fat dietary intervention the excess supply of fat does, in fact, inhibit the process of DNL [106]. In accordance, Hijmans et al [70] predicted with a novel computational modelling approach that not increased DNL but the amount of FA influx determines hepatic fat load.…”

Section: Discussionmentioning

confidence: 84%

Zonation of hepatic fat accumulation: insights from mathematical modelling of nutrient gradients and fatty acid uptake

Schleicher

Dahmen

Guthke

et al. 2017

J. R. Soc. Interface.

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Intrinsic of non-alcoholic fatty liver diseases is an aberrant accumulation of triglycerides (steatosis), which occurs inhomogeneously within lobules. To improve our understanding of the mechanisms involved in this zonation patterning, we developed a mathematical multicompartment model of hepatic fatty acid metabolism accompanied by blood flow simulations. A model analysis determines the influence of the uptake process of fatty acids, the porto-central gradient of plasma fatty acid concentration, and the oxygen supply via blood on the zonation of triglyceride accumulation. From this theoretical perspective, the plasma oxygen gradient, but not the fatty acid gradient, leads the way to a zonated triglyceride accumulation by its decisive role in oxidative processes. In addition, the uptake mechanism of fatty acids seems to be fundamental for a pericentral dominance of steatosis. However, the mechanism of cellular fatty acid uptake from the blood is still under debate. Our theoretical approach supports the transporter-mediated uptake mechanism and reveals that the maximal velocity of fatty acid uptake affects the switching between a periportal and a pericentral triglyceride accumulation. Further research on hepatic fatty acid uptake is needed to push forward our understanding of aberrant triglyceride accumulation in diet-induced steatosis.

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Section: Discussionmentioning

confidence: 84%

Zonation of hepatic fat accumulation: insights from mathematical modelling of nutrient gradients and fatty acid uptake

Schleicher

Dahmen

Guthke

et al. 2017

J. R. Soc. Interface.

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“…In this study, rats, a commonly used experimental model to investigate obesity, were fed a diet with a high amount (35%) of dietary fat. The diet was also low in polyunsaturated fatty acids (PUFA) in order to prevent the strong inhibitory effect of hepatic fatty acid synthesis by these unsaturated fatty acids (Ferramosca & Zara, ; Ferramosca et al ., ). The administration of this diet caused a significant increase in body weight and predisposed rats to obesity.…”

Section: Discussionmentioning

confidence: 97%

“…‘Metabolic syndrome’ is a disorder of energy use and storage, characterized by the presence of different risk factors, such as abdominal obesity, dyslipidemia, insulin resistance, and a pro‐inflammatory state. The prevalence of this syndrome is increasing worldwide, along with its related complications, such as cardiovascular diseases, type 2 diabetes, and liver alterations (Ferramosca et al ., ; Jahangir et al ., ; Asrih & Jornayvaz, ; Yoo & Choi, ).…”

Section: Introductionmentioning

confidence: 99%

A high‐fat diet negatively affects rat sperm mitochondrial respiration

et al. 2016

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SUMMARYRecent evidences have linked abdominal obesity, insulin resistance, and dyslipidemia to male infertility. Since a defective energy metabolism may play an important role in the impairment of sperm quality, the aim of this study is to investigate the sperm energetic metabolism in rats fed with a high-fat diet, an animal model associated with metabolic syndrome development. Sexually mature male Sprague-Dawley rats were divided into two groups and fed for 4 weeks a standard diet (control group) or a diet enriched in 35% of fat (high fat group). Liver and adipose tissue weight, plasma glucose, insulin, and lipid concentrations were determined. Activities of enzymes involved in sperm energetic metabolism were evaluated by spectrophotometric assays. Sperm mitochondrial respiratory activity was evaluated with a polarographic assay of oxygen consumption. The administration of a high-fat diet caused a significant increase in body weight of rats and provoked hyperglycemia, hyperinsulinemia, and dyslipidemia. In these animals, we also observed a reduction in sperm concentration and motility. The investigation of sperm energetic metabolism in animals fed a high-fat diet revealed an impairment in the activity of pyruvate and lactate dehydrogenase, citrate synthase, and respiratory chain complexes. A parallel reduction in the cellular levels of adenosine triphosphate (ATP) and an increase in oxidative damage were also observed. A defective energy metabolism may play an important role in the impairment of sperm quality in the high-fat diet fed rats.

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“…Several lines of evidence indicate that structural and functional alterations in mitochondria are crucial to the development of NAFLD[18-20]. The structural alterations include depletion of mitochondrial DNA (mtDNA), as well as morphological and ultrastructural changes, whereas the functional alterations include defects in mitochondrial β-oxidation and respiration[21].…”

Section: Dysfunctional Mitochondrial Bioenergetics In the Fatty Livermentioning

confidence: 99%

Antioxidant dietary approach in treatment of fatty liver: New insights and updates

Ferramosca

Giacomo

Zara

2017

WJG

Self Cite

145

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Non-alcoholic fatty liver disease (NAFLD) is a common clinicopathological condition, encompassing a range of conditions caused by lipid deposition within liver cells. To date, no approved drugs are available for the treatment of NAFLD, despite the fact that it represents a serious and growing clinical problem in the Western world. Identification of the molecular mechanisms leading to NAFLD-related fat accumulation, mitochondrial dysfunction and oxidative balance impairment facilitates the development of specific interventions aimed at preventing the progression of hepatic steatosis. In this review, we focus our attention on the role of dysfunctions in mitochondrial bioenergetics in the pathogenesis of fatty liver. Major data from the literature about the mitochondrial targeting of some antioxidant molecules as a potential treatment for hepatic steatosis are described and critically analysed. There is ample evidence of the positive effects of several classes of antioxidants, such as polyphenols (i.e., resveratrol, quercetin, coumestrol, anthocyanins, epigallocatechin gallate and curcumin), carotenoids (i.e., lycopene, astaxanthin and fucoxanthin) and glucosinolates (i.e., glucoraphanin, sulforaphane, sinigrin and allyl-isothiocyanate), on the reversion of fatty liver. Although the mechanism of action is not yet fully elucidated, in some cases an indirect interaction with mitochondrial metabolism is expected. We believe that such knowledge will eventually translate into the development of novel therapeutic approaches for fatty liver.

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Differential effects of high-carbohydrate and high-fat diets on hepatic lipogenesis in rats

Cited by 45 publications

References 38 publications

Zonation of hepatic fat accumulation: insights from mathematical modelling of nutrient gradients and fatty acid uptake

Zonation of hepatic fat accumulation: insights from mathematical modelling of nutrient gradients and fatty acid uptake

A high‐fat diet negatively affects rat sperm mitochondrial respiration

Antioxidant dietary approach in treatment of fatty liver: New insights and updates

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