Differential effects of environmental chemicals and food contaminants on adipogenesis, biomarker release and PPARγ activation

The effects of phthalate and nonylphenol exposure on body size and secondary sexual characteristics during puberty

“…The activation of PPAR␥ promotes the differentiation of preadipose cells into adipocytes (Feige et al, 2007). Taxvig et al showed that MEHP caused increased adipogenesis (Taxvig et al, 2012). The adipogenic effect of PAEs might occur through the activation of PPAR␥ or the inhibition of TSH.…”

“…Epidemiological studies have shown positive associations between MEHP and adipogenesis (Taxvig et al, 2012), LMW metabolites and BMI (Trasande et al, 2013) and MEHP and MEP and WC in Chinese school children . MBzP and MEHP act as an obesogen by activating of peroxisome proliferatoractivated receptors (PPARs) (Hurst and Waxman, 2003).…”

1,25-Dihydroxyvitamin D3/vitamin D receptor suppresses brown adipocyte differentiation and mitochondrial respiration

“…Brown preadipocytes were transiently transfected with PPARc transactivation reporter system [20], in which murine PPARc ligand binding domain is coupled to the Gal4 DNA binding domain to form mPPARc-Gal4 and a reporter construct containing an upstream activating sequence (UAS)-linked luciferase, 4xUAS-TK-luc (TK: thymidine kinase) and b-galactosidase expression plasmid. PPARc transactivation system was a gift from Dr. Susanne Mandrup at University of Southern Denmark, Denmark.…”

Perfluorooctanoic acid binds to peroxisome proliferator-activated receptor γ and promotes adipocyte differentiation in 3T3-L1 adipocytes

“…In addition, no false positive of the binding assay for PPAR γ was observed (data not shown). Taxvig et al 14) reported that 0.3−30 μM PFOA did not induce PPAR γ activation, while 100 μM PFOA tended to increase the activation. These results suggest the possibility that PFOA at a high concentration acted as an agonistic ligand of PPAR γ.…”