Arteriosclerosis

1990

DOI: 10.1161/01.atv.10.4.616

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Differential effect of Photofrin II on growth of human smooth muscle cells from nonatherosclerotic arteries and atheromatous plaques in vitro.

Peter C. Dartsch

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Thomas Ischinger

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Cardiovascular Applications Of Pdt In Vitro Experience1

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Cited by 29 publications

(14 citation statements)

References 34 publications

(11 reference statements)

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“…In addition, after 4 d in culture, the proliferation rate of the cholesterol-SMC in culture was twofold compared to the control-SMC, as measured by the number of cells. This difference in the proliferation is comparable with that described elsewhere between human SMC from nonatherosclerotic arteries and atheromatous plaques (48).…”

Section: Figsupporting

confidence: 91%

Changes in cultured arterial smooth muscle cells isolated from chicks upon cholesterol feeding

et al. 1998

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We have developed cultures of smooth muscle cells (SMC) isolated from arterial hypercholesterolemic chicks (cholesterol-SMC). These cultures are suitable for the study at the molecular level of the changes in arterial SMC induced by a cholesterol diet. By using a strong dose of cholesterol (5%) for 10 d, we obtained very proliferative SMC which became foam cells after 30 d in culture. On the other hand, SMC cultures isolated from control-fed chicks had a lower growth rate than the SMC ones under the same culture conditions. DNA synthesis was fourfold greater in cholesterol-SMC than in control-SMC cultures. Intracellular cholesterol concentrations were the same in both cholesterol and control SMC during the first 14 d of culture but afterward increased in differing ways: after 20 d of culture the cholesterol-SMC increased their cholesterol content to double the control. We give here the results obtained from transmission electron microscopy, lipid analysis, proliferation studies, DNA, RNA and protein synthesis, and then discuss their implications.

“…In addition, after 4 d in culture, the proliferation rate of the cholesterol-SMC in culture was twofold compared to the control-SMC, as measured by the number of cells. This difference in the proliferation is comparable with that described elsewhere between human SMC from nonatherosclerotic arteries and atheromatous plaques (48).…”

Section: Figsupporting

confidence: 91%

Changes in cultured arterial smooth muscle cells isolated from chicks upon cholesterol feeding

et al. 1998

View full text Add to dashboard Cite

We have developed cultures of smooth muscle cells (SMC) isolated from arterial hypercholesterolemic chicks (cholesterol-SMC). These cultures are suitable for the study at the molecular level of the changes in arterial SMC induced by a cholesterol diet. By using a strong dose of cholesterol (5%) for 10 d, we obtained very proliferative SMC which became foam cells after 30 d in culture. On the other hand, SMC cultures isolated from control-fed chicks had a lower growth rate than the SMC ones under the same culture conditions. DNA synthesis was fourfold greater in cholesterol-SMC than in control-SMC cultures. Intracellular cholesterol concentrations were the same in both cholesterol and control SMC during the first 14 d of culture but afterward increased in differing ways: after 20 d of culture the cholesterol-SMC increased their cholesterol content to double the control. We give here the results obtained from transmission electron microscopy, lipid analysis, proliferation studies, DNA, RNA and protein synthesis, and then discuss their implications.

“…PDT with Photofrin II substantially impairs the growth of cultured SMCs derived from both atherosclerotic lesions and nonatherosclerotic arteries. 30,47 The effect on SMCs obtained from atherosclerotic lesions (activated SMCs) is much greater than that seen in the cells obtained from the normal vascular wall. Similarly, studies of SMCs derived from saphenous vein grafts have shown significant inhibition of their growth after photosensitization.…”

Section: Cardiovascular Applications Of Pdt In Vitro Experiencementioning

confidence: 96%

Photoangioplasty

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et al. 2000

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Abstract-Photodynamic therapy (PDT) has been studied and applied to various disease processes. The potential of PDT for selective destruction of target tissues is especially appealing in cardiovascular disease, in which other existing interventional tools are somewhat nonselective and carry substantial risk of damage to the normal arterial wall. Enthusiasm for photoangioplasty (PDT of vascular de novo atherosclerotic and, potentially, restenotic lesions) is fueled by more effective second-generation photosensitizers and technological advances in endovascular light delivery. This excitement revolves around at least 4 significant attributes of light-activated therapy: the putative selectivity and safety of photoangioplasty, the potential for atraumatic and effective debulking of atheromatous plaque through a biological mechanism, the postulated capability to reduce or inhibit restenosis, and the potential to treat long segments of abnormal vessel by simply using fibers with longer light-emitting regions. The available nonclinical data, coupled with the observations of a new phase I trial in human peripheral atherosclerosis, suggest a promising future for photoangioplasty in the treatment of primary atherosclerosis and prevention of restenosis. (Circulation. 2000;102:591-596.)

“…In addition, it is showing great promise in the treatment and management of a variety of non-malignant conditions such as dysfunctional uterine bleeding [3], macular degeneration [4,5], dermatological port-wine stains [6], psoriasis [7,8] and atherosclerosis [9,10].…”

Section: Introductionmentioning

confidence: 99%

Chromosomes are target sites for photodynamic therapy as demonstrated by subcellular laser microirradiation

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et al. 2000

Journal of Photochemistry and Photobiology B: Biology

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