Chromosomes are target sites for photodynamic therapy as demonstrated by subcellular laser microirradiation

Luan, Hong; Do, Tuyen; Kasravi, Sanaz; Aurasteh, P.; Nguyen, Andrea; Huang, Andy; Wang, Zifu; Berns, Michael W.

doi:10.1016/s1011-1344(00)00013-0

Cited by 7 publications

(3 citation statements)

References 24 publications

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“…Laser microbeams have been used in experimental biology and ophthalmology for decades [31,32], but no therapeutic microbeam devices have been developed for skin surgery or dermatology. By adjusting focal depth and pulse energy, different tissue compartments can be arbitrarily chosen as primary sites for photothermal lesions.…”

Section: Discussionmentioning

confidence: 99%

Fractional Photothermolysis: A New Concept for Cutaneous Remodeling Using Microscopic Patterns of Thermal Injury

Manstein¹,

Herron²,

Sink³

et al. 2004

Lasers Surg Med

1,381

1,236

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Background and Objectives: We introduce and clinically examine a new concept of skin treatment called fractional photothermolysis (FP), achieved by applying an array of microscopic treatment zones (MTZ) of thermal injury to the skin. Study Design/Materials and Methods: Two prototype devices emitting at 1.5 mm wavelength provided a pattern of micro-exposures with variable MTZ density. Effects of different MTZ densities were tested on the forearms of 15 subjects. Clinical effects and histology were assessed up to 3 months after exposure. Treatment of photoaged skin on the periorbital area in an additional 30 subjects receiving four treatments over a period of 2-3 weeks was also tested. Tissue shrinkage and clinical effects were assessed up to 3 months after treatment. Results: Pattern densities with spacing of 250 mm or more were well tolerated. Typical MTZ had a diameter of 100 mm and penetrated 300 mm into the skin. Reepithelialization was complete within 1 day. Clinical effects were assessed over a 3-month period. Histology at 3 months revealed enhanced undulating rete ridges and increased mucin deposition within the superficial dermis. Periorbital treatments were well tolerated with minimal erythema and edema. Linear shrinkage of 2.1% was measured 3 months after the last treatment. The wrinkle score improved 18% (P < 0.001) 3 months after the last treatment. Conclusions: FP is a new concept for skin restoration treatment. Safety and efficacy were demonstrated with a prototype device. Further clinical studies are necessary to refine the optimum parameters and to explore further dermatological applications.

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Section: Discussionmentioning

confidence: 99%

Fractional Photothermolysis: A New Concept for Cutaneous Remodeling Using Microscopic Patterns of Thermal Injury

Manstein¹,

Herron²,

Sink³

et al. 2004

Lasers Surg Med

1,381

1,236

View full text Add to dashboard Cite

show abstract

“…Weitere, neuere Anwendungen ergeben sich durch eine Extrapolation von früher erwähnten In-vitro-und In-vivo-Verfahren [20], indem Nanopartikel, wie z. B. photodynamische Substanzen [63], mittels geeigneter Trägersysteme, wie zum Beispiel durch Antikörper oder Lipoproteine, an entsprechende Zielstrukturen gebracht werden. Eine Zerstörung bestimmter Rezeptoren und Zellorganellen sowie der Ersatz von unerwünschtem Genmaterial durch geeignetes Material [25] eröffnet Möglichkeiten, nicht nur die Zellorganellen zu zerstören, sondern auch gezielt Funktionen zu modifizieren.…”

Section: Wie Lautet Die Zukunft?unclassified

Mikroskopgestützte Laserchirurgie - zelluläre Laserchirurgie in der Augenheilkunde. Eine Übersicht12

Fankhauser¹

2001

Klin Monatsbl Augenheilkd

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This is a review of a number of laser methods, allowing to realize strongly localized, atraumatic, molecular, cellular, and subcellular microeffects. New instruments are the laser tweezers and the laser knife to be used with a microscope (microscopical surgery). Laser tweezers allow fixation, by means of the radiation pressure of molecular, cellular, and subcellular elements, of diameters as small as 1 microm. These may be irradiated atraumatically by means of short-pulsed lasers. A reversible damage of the cell membrane allows to introduce living genetic material into living cells and to reversibly modify their genetic behaviour. Micromanipulation/irradiation of living cells by means of the short-pulse laser method of living cells may be optimal in in-vivo fertilization experiments. Short-pulse laser methods have been tentatively introduced in ophthalmology and highly localized effects have been realized in the trabecular meshwork for the treatment of glaucoma and in the retina in retinal pathology.

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“…5 There has been a particular interest in events that occur immediately after the selective production of singlet oxygen in a given subcellular domain. Pertinent experiments can be performed at the level of a single cell, and include the use of (1) sensitizers that predominantly localize in a given organelle (e.g., the mitochondria), 5,7- 12 (2) sensitizer irradiation using a laser beam specifically focused into a subcellular domain, 13,14 and (3) fluorescence-based assays that probe a specific response (e.g., cytoskeletal collapse). 2, 15 For the present study, we set out to perform complementary single cell experiments that assess the effect of intracellular singlet oxygen production on the ion permeability of the cell membrane.…”

Section: Introductionmentioning

confidence: 99%

Effect of intracellular photosensitized singlet oxygen production on the electrophysiological properties of cultured rat hippocampal neurons

Breitenbach

Ogilby

Lambert

2010

Photochem Photobiol Sci

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Whole-cell patch-clamp recordings from single cultured mammalian neurons have been used to provide insight into early membrane-dependent events that result upon the intracellular photosensitized production of singlet molecular oxygen, O(2)(a(1)Δ(g)). The singlet oxygen sensitizers used, pyropheophorbide a (PPa) and protoporphyrin IX (PpIX), locate mainly in cell membranes and mitochondria, respectively. Irradiation of these sensitizers altered both passive and dynamic electrophysiological properties of the neurons in a dose-dependent manner, though the response threshold was much lower with PPa than with PpIX. In particular, notable decreases were observed in the rising and falling rates of action potentials and, at higher light fluences, plateau potentials consistent with activation of Ca(2+) channels also developed. The data suggest that singlet oxygen production specifically influences Na(+), K(+) and Ca(2+) ionophores in the cell membrane. Upon terminating sensitizer irradiation, responses evoked by PPa stabilized immediately whereas those evoked by PpIX continued to develop. These data are consistent with a spatially-resolved sphere of intracellular singlet oxygen activity. While the response to PPa irradiation appears to be membrane specific, the response to PpIX irradiation appears to be systemic and possibly part of a cascade of apoptotic events. These results should contribute to a better understanding of membrane-dependent events pertinent to cell death mediated by singlet oxygen.

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Chromosomes are target sites for photodynamic therapy as demonstrated by subcellular laser microirradiation

Cited by 7 publications

References 24 publications

Fractional Photothermolysis: A New Concept for Cutaneous Remodeling Using Microscopic Patterns of Thermal Injury

Fractional Photothermolysis: A New Concept for Cutaneous Remodeling Using Microscopic Patterns of Thermal Injury

Mikroskopgestützte Laserchirurgie - zelluläre Laserchirurgie in der Augenheilkunde. Eine Übersicht12

Effect of intracellular photosensitized singlet oxygen production on the electrophysiological properties of cultured rat hippocampal neurons

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