Differential effect of hormone therapy on E1S-sulfatase activity in non-malignant and cancerous breast cells in vitro

Stute, Petra; Götte, Martin; Kiesel, L.

doi:10.1007/s10549-007-9615-7

Cited by 11 publications

(15 citation statements)

References 66 publications

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“…Previous studies in BC cell lines have demonstrated that treatment with estradiol, and various progestins, respectively, rather affect enzyme activity than its protein levels and mRNA expression [40,59]. We previously reported similar effects in nonmalignant human breast cells in vitro [45]. Thus, the enzymatic effects leading to a decreased E 1 and E 2 formation could be mediated by a decreased STS, elevated sulfotransferase, or by a shift in the balance of 17␤-HSD type 1 and 2 activity.…”

Section: Discussionsupporting

confidence: 58%

“…STS activity was measured as described previously [45,46]. In brief, breast tissue (150 mg) was finely minced with scissors and homogenized on ice, in 2 ml 0.06 M Tris-HCl-buffer pH 7.0 (assay buffer throughout the entire procedure) for approximately 1 min at maximum speed using a Polytron homogenizer.…”

Section: E 1 S-sulfatase (Sts) Activity Assaymentioning

confidence: 99%

“…Estradiol and various progestogens have been shown to influence STS in BC cells in vitro [38][39][40][41][42][43][44]. In vitro, the effect of steroids on local estrogen formation in normal and malignant breast cells is rather due to changes in STS activity than in its mRNA and protein levels [45]. We recently demonstrated that longterm HT alters local estrogen formation in breast tissue of postmenopausal cynomolgus monkeys (Macaca fascicularis) and women depending on breast tissue composition [46,47].…”

Section: Introductionmentioning

confidence: 99%

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Effects of black cohosh on estrogen biosynthesis in normal breast tissue in vitro

Stute¹,

Nisslein

Götte³

et al. 2007

Maturitas

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Objectives: To investigate the effect of black cohosh on the estrogen biosynthesis in the breast in vitro. Methods: Steroid sulfatase (STS) activity was studied in normal breast tissue obtained from pre-and postmenopausal women undergoing reduction mammoplasty. STS protein expression was studied by immunohistochemistry and western blotting. Breast tissue was incubated in vitro without or with black cohosh (iCR) at concentrations ranging from 0.1 mg/ml to 1 ng/ml. STS activity was evaluated by incubating homogenized breast tissue with [ 3 H]-estrone sulfate, separating the formed products, estrone (E 1 ) and estradiol (E 2 ), by thin layer chromatography and measuring the amounts of E 1 and E 2 by scintillation counting. Results: STS protein expression and enzymatic activity were detected in all specimens investigated. In all groups, significantly more E 1 than E 2 was produced. Local estrogen formation was decreased in premenopausal breast tissue by treatment with iCR at 0.1 mg/ml (p ≤ 0.05). Conclusions: iCR decreases local estrogen formation in normal human breast tissue in vitro. This may contribute to the lack of hormonal effects of black cohosh in breast tissue observed in previous studies.

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Section: Discussionsupporting

confidence: 58%

Section: E 1 S-sulfatase (Sts) Activity Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of black cohosh on estrogen biosynthesis in normal breast tissue in vitro

Stute¹,

Nisslein

Götte³

et al. 2007

Maturitas

Self Cite

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“…Our finding that STS activity was higher in malignant than healthy cells and tissue, respectively, is in accordance with previous studies in breast cells [15] and brain tumors [17]. Other factors might also contribute to the differences observed such as species (human vs. monkey), origin of investigated material (SH-SY5Y cells obtained from bone marrow biopsy vs. hippocampus tissue obtained from brain necropsy), culture conditions (cells vs. tissue), and the hormonal environment.…”

Section: Discussionsupporting

confidence: 92%

“…STS activity was measured as described previously [15]. In brief, SH-SY5Y cells were homogenized on ice, in 2 ml 0.06 M Tris-HCl-buffer pH 7.0 (assay buffer throughout the entire procedure) for approximately 1 min at maximum speed using a Polytron® homogenizer (Kinematica, AG).…”

Section: E1s-sulfatase (Sts) Activity Assaymentioning

confidence: 99%

Effects of black cohosh on estrogen biosynthesis in hippocampus of non-human primates ex vivo in vitro and in human neuroblastoma cells in vitro

et al. 2016

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Background: Estrogens have neuroprotective properties. The aim of the study was to assess the impact of an isopropanolic extract of Cimicifuga racemosa (iCR), 17ß-estradiol (E2), testosterone (T) and Tibolone's 3α-OH metabolite (Org4094) on local estrogen formation in hippocampus tissue from non-human primates ex vivo in vitro and human neuroblastoma cells (SH-SY5Y) in vitro. Methods: SH-SY5Y cells were incubated in RPMI 1640 medium containing 5 % steroid-depleted fetal calf serum for 3 days, and subsequently incubated in absence or presence of iCR at 10 μg/ml (n = 5) and 1 μg/ml (n = 5), E2 at 10 −8 M (n = 5), and 10 −6 M (n = 5), or T at 10 −8 M (n = 5), and 10 −6 M (n = 5), respectively, at 37°C for either 24 h or directly in cell extracts. Hippocampus tissue from healthy female cynomolgus macaques (n = 14) was homogenized and treated with iCR, E2 and Org4049 accordingly. STS activity was evaluated by incubating homogenized brain cells and tissue with [3H]-estrone sulfate and separating the products estrone (E1) and E2 by thin layer chromatography. STS activity was expressed as total estrogen formation (E1 + E2) fmol/mg of protein/min. Statistical comparisons were made using unpaired T-Test for comparing two sets of data and ANOVA to compare many groups at once. A p value < 0.05 was considered to be significant different. Results: Basal total estrogen formation was significantly higher in proliferative SH-SY5Y cells (1350.06 ± 109 E1 + E2 fmol/mg of protein/min) compared to hippocampus tissue (37.83 ± 3.9 E1 + E2 fmol/mg of protein/min; p < 0.01). ICR exerted a bidirectional action with an immediate strong inhibitory effect being followed by a stimulation of STS activity (85 % increase). E2 had a bidirectional dose-dependent long-term effect on STS activity (−23 % reduction and 18 % increase). T and Org4094 had an immediate strong inhibitory effect on STS activity that further maintained with 24 h T treatment (30 % decrease). Conclusions: Local estrogen formation within brain cells and tissue may be modulated by black cohosh, 17ß-estradiol, testosterone, and Tibolone's 3α-OH metabolite, respectively. The clinical implication of this finding has to be elucidated.

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Effect of testosterone on E1S-sulfatase activity in non-malignant and cancerous breast cells in vitro

Götte¹,

Kalkhake²,

Ploeger³

et al. 2009

The Journal of Steroid Biochemistry and Molecular Biology

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Differential effect of hormone therapy on E1S-sulfatase activity in non-malignant and cancerous breast cells in vitro

Cited by 11 publications

References 66 publications

Effects of black cohosh on estrogen biosynthesis in normal breast tissue in vitro

Effects of black cohosh on estrogen biosynthesis in normal breast tissue in vitro

Effects of black cohosh on estrogen biosynthesis in hippocampus of non-human primates ex vivo in vitro and in human neuroblastoma cells in vitro

Effect of testosterone on E1S-sulfatase activity in non-malignant and cancerous breast cells in vitro

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