Effect of testosterone on E1S-sulfatase activity in non-malignant and cancerous breast cells in vitro

Götte, Martin; Kalkhake, Kathrin; Ploeger, Sebastian; Kiesel, L.; Stute, Petra

doi:10.1016/j.jsbmb.2009.09.007

Cited by 6 publications

(7 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…By "direct" incubation, we believe to obtain more valid information about regulation of the selected compounds at the level of the enzyme's catalytic activity. Our findings in brain are in agreement with previous studies in malignant breast cells and normal mammary gland, demonstrating an inhibitory effect on STS activity after "direct" incubation by iCR [18], E2 [15], T [19] and Org4094 [15], respectively.…”

Section: Discussionsupporting

confidence: 93%

See 1 more Smart Citation

Effects of black cohosh on estrogen biosynthesis in hippocampus of non-human primates ex vivo in vitro and in human neuroblastoma cells in vitro

et al. 2016

Self Cite

View full text Add to dashboard Cite

Background: Estrogens have neuroprotective properties. The aim of the study was to assess the impact of an isopropanolic extract of Cimicifuga racemosa (iCR), 17ß-estradiol (E2), testosterone (T) and Tibolone's 3α-OH metabolite (Org4094) on local estrogen formation in hippocampus tissue from non-human primates ex vivo in vitro and human neuroblastoma cells (SH-SY5Y) in vitro. Methods: SH-SY5Y cells were incubated in RPMI 1640 medium containing 5 % steroid-depleted fetal calf serum for 3 days, and subsequently incubated in absence or presence of iCR at 10 μg/ml (n = 5) and 1 μg/ml (n = 5), E2 at 10 −8 M (n = 5), and 10 −6 M (n = 5), or T at 10 −8 M (n = 5), and 10 −6 M (n = 5), respectively, at 37°C for either 24 h or directly in cell extracts. Hippocampus tissue from healthy female cynomolgus macaques (n = 14) was homogenized and treated with iCR, E2 and Org4049 accordingly. STS activity was evaluated by incubating homogenized brain cells and tissue with [3H]-estrone sulfate and separating the products estrone (E1) and E2 by thin layer chromatography. STS activity was expressed as total estrogen formation (E1 + E2) fmol/mg of protein/min. Statistical comparisons were made using unpaired T-Test for comparing two sets of data and ANOVA to compare many groups at once. A p value < 0.05 was considered to be significant different. Results: Basal total estrogen formation was significantly higher in proliferative SH-SY5Y cells (1350.06 ± 109 E1 + E2 fmol/mg of protein/min) compared to hippocampus tissue (37.83 ± 3.9 E1 + E2 fmol/mg of protein/min; p < 0.01). ICR exerted a bidirectional action with an immediate strong inhibitory effect being followed by a stimulation of STS activity (85 % increase). E2 had a bidirectional dose-dependent long-term effect on STS activity (−23 % reduction and 18 % increase). T and Org4094 had an immediate strong inhibitory effect on STS activity that further maintained with 24 h T treatment (30 % decrease). Conclusions: Local estrogen formation within brain cells and tissue may be modulated by black cohosh, 17ß-estradiol, testosterone, and Tibolone's 3α-OH metabolite, respectively. The clinical implication of this finding has to be elucidated.

show abstract

Section: Discussionsupporting

confidence: 93%

“…Our results contribute to our previous work showing that local STS activity can be modulated by exogenous steroids and black cohosh [15,18,19,34] -also in brain (this study). This might be one explanation as to how those compounds frequently used in menopausal women may affect cognition.…”

Section: Discussionsupporting

confidence: 87%

Effects of black cohosh on estrogen biosynthesis in hippocampus of non-human primates ex vivo in vitro and in human neuroblastoma cells in vitro

et al. 2016

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, the extent of enzyme inhibition was tissuedependent, and mammary 17bHSD1 seemed to be more sensitive to co-incubated testosterone although this difference was not significant. Apart from 17bHSD1, previous studies found an inhibitory effect of testosterone on mammary aromatase 43 , and STS activity 23 , respectively. These in vitro findings support the hypothesis of an anti-estrogenic effect of testosterone on the mammary gland, which has previously been postulated [44][45][46] .…”

Section: Discussionmentioning

confidence: 92%

“…For the PA, the conversion of the co-substrate NADH to NAD+ in the presence of the substrate E1 was used to determine E2 formation 35 . Therefore, 60 ml enzyme solution in assay buffer (0.2 M Tris-HCl, 0.1 M Na 2 HPO 4 , pH 6.9) were mixed with 30 ml substrate (381 mg/ml E1 in 95% ethanol), and 60 ml NADH (1 mg/ml) in assay buffer in the presence or absence of the following substances 23,31 ( Figure 1):…”

Section: Human Placenta and Mammary Glandmentioning

confidence: 99%

See 1 more Smart Citation

The impact of testosterone, tibolone and black cohosh on purified mammary and placental 17β-hydroxysteroid dehydrogenase type 1

Stute

Kalkhake

Kiesel³

et al. 2014

Journal of Enzyme Inhibition and Medicinal Chemistry

Self Cite

View full text Add to dashboard Cite

Context: Mammary and placental 17b-hydroxysteroid dehydrogenase type 1 (17bHSD1). Objective: To assess the impact of testosterone, tibolone, and black cohosh on purified mammary and placental 17bHSD1. Materials and methods: 17bHSD1 was purified from human mammary gland and placenta by column chromatography, its activity was monitored by a radioactive activity assay, and the degree of purification was determined by gel electrophoresis. Photometric cofactor transformation analysis was performed to assess 17bHSD1 activity without or in presence of testosterone, tibolone and black cohosh. Results: 17bHSD1 from both sources displayed a comparable basal activity. Testosterone and tibolone metabolites inhibited purified mammary and placental 17bHSD1 activity to a different extent, whereas black cohosh had no impact. Discussion: Studies on purified enzymes reveal the individual action of drugs on local regulatory mechanisms thus helping to develop more targeted therapeutic intervention. Conclusion: Testosterone, tibolone and black cohosh display a beneficial effect on local mammary estrogen metabolism by not affecting or decreasing local estradiol exposure.

show abstract

Discrimination of wild and domestic deer musk using isotope ratio mass spectrometry

Wang

et al. 2018

J Mass Spectrom

View full text Add to dashboard Cite

Musk is the dried secretion of the musk pod (sac) of adult male musk deer, and has been used as perfume and traditional medicine for thousands of years. Steroid was regarded as 1 of the most important active compounds in musk. In order to protect the wild musk deer, musk deer farming has been carried out in China, India, and Nepal. However, it is hard to differentiate the 2 origins of musk by morphological identification. No other method has been reported so far for the discrimination of wild and domestic deer musk. The establishment of a reliable discrimination method has become an urgent work. In the present study, 6 batches of wild deer musk and 14 batches of domestic deer musk were collected. Analysis of steroid components in musk was carried out with GC-MS/MS. Androgen, progestin, estrogen, and sterol were detected in those samples. Large diversity was observed in the concentrations of steroids in musk. No obvious difference could be observed in steroid concentrations between wild and domestic deer musk by principal component analysis and cluster analysis. Furthermore, the δ C values of steroids were determined by gas chromatography/combustion coupled with isotopic ratio mass spectrometry. There were significant differences (P < .01) in steroid δ C values between wild origin musk and domesticated origin musk. Isotopic ratio mass spectrometry can be used to discriminate wild and domestic deer musk.

show abstract

Effect of testosterone on E1S-sulfatase activity in non-malignant and cancerous breast cells in vitro

Cited by 6 publications

References 62 publications

Effects of black cohosh on estrogen biosynthesis in hippocampus of non-human primates ex vivo in vitro and in human neuroblastoma cells in vitro

Effects of black cohosh on estrogen biosynthesis in hippocampus of non-human primates ex vivo in vitro and in human neuroblastoma cells in vitro

The impact of testosterone, tibolone and black cohosh on purified mammary and placental 17β-hydroxysteroid dehydrogenase type 1

Discrimination of wild and domestic deer musk using isotope ratio mass spectrometry

Contact Info

Product

Resources

About