Differential effect of heat shock on RNA metabolism in human Burkitt's lymphoma B-cell lines

Kumar, Ashok; Kurl, Rabinder N.; Kryworuchko, Marko; Díaz‐Mitoma, Francisco; Sharma, Surendra

doi:10.1016/0145-2126(95)00063-1

Cited by 4 publications

(2 citation statements)

References 35 publications

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“…As the tumour suppressor BARD1 is involved in this response, it is possible that malignant cells show altered levels of polyadenylation of certain mRNAs. Supporting this idea, enhanced polyadenylation has been detected in certain tumour cells (Kumar et al , 1995; Scorilas, 2002), polyadenylation is inactivated in the M‐phase (Colgan et al , 1996, 1998) and expression levels of poly(A) polymerase can interfere with cell growth (Zhao and Manley, 1998). Moreover, antiproliferative transcription factors, such as BTG2 and TOB, have been shown to enhance deadenylation and subsequent mRNA decay (Ezzeddine et al , 2007; Mauxion et al , 2008).…”

Section: Discussionmentioning

confidence: 98%

Nuclear deadenylation/polyadenylation factors regulate 3′ processing in response to DNA damage

Cevher

Zhang

Fernandez

et al. 2010

EMBO J

138

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We previously showed that mRNA 3 0 end cleavage reaction in cell extracts is strongly but transiently inhibited under DNA-damaging conditions. The cleavage stimulation factor-50 (CstF-50) has a role in this response, providing a link between transcription-coupled RNA processing and DNA repair. In this study, we show that CstF-50 interacts with nuclear poly(A)-specific ribonuclease (PARN) using in vitro and in extracts of UV-exposed cells. The CstF-50/ PARN complex formation has a role in the inhibition of 3 0 cleavage and activation of deadenylation upon DNA damage. Extending these results, we found that the tumour suppressor BARD1, which is involved in the UV-induced inhibition of 3 0 cleavage, strongly activates deadenylation by PARN in the presence of CstF-50, and that CstF-50/ BARD1 can revert the cap-binding protein-80 (CBP80)-mediated inhibition of PARN activity. We also provide evidence that PARN along with the CstF/BARD1 complex participates in the regulation of endogenous transcripts under DNA-damaging conditions. We speculate that the interplay between polyadenylation, deadenylation and tumour-suppressor factors might prevent the expression of prematurely terminated messengers, contributing to control of gene expression under different cellular conditions.

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Section: Discussionmentioning

confidence: 98%

Nuclear deadenylation/polyadenylation factors regulate 3′ processing in response to DNA damage

Cevher

Zhang

Fernandez

et al. 2010

EMBO J

138

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“…Polyadenylation plays a key role in regulating Hsp70 expression [28]and could be potentially important to control the expression of other transcripts expressed during heat shock. One observation reports an enhanced polyadenylation in response to thermal stress after infection of human B cells with Epstein‐Barr virus [29]. In addition to a possible increase in the stability of the transcripts, polyadenylation enhances the efficiency of translation and the efficiency of mRNA export from the nucleus [30, 31].…”

Section: Discussionmentioning

confidence: 99%

Marked differences between avian and mammalian testicular cells in the heat shock induction and polyadenylation of Hsp70 and ubiquitin transcripts

Mezquita¹,

Mezquita²,

Mezquita³

1998

FEBS Letters

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Mammalian male germ cells undergo apoptosis at the body's internal temperature of 37³C. Birds, however, are unique among homeothermic animals in developing spermatogenesis at the elevated avian internal body temperature of 40^41³C. To shed light on the mechanisms that maintain an efficient avian spermatogenesis at elevated temperatures we compared, in mouse and chicken testicular cells, the expression of genes that are essential for stress resistance: Hsp70 and ubiquitin. While the expression of Hsp70 and ubiquitin did not change upon heat shock in mouse testicular cells, both the amount and polyadenylation of Hsp70 and ubiquitin transcripts increased when male germ cells from adult chicken testis were exposed to elevated temperatures.z 1998 Federation of European Biochemical Societies.

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Differential Effect of IL-4 and IL-13 on CD44 Expression in the Burkitt's Lymphoma B Cell Line BL30/B95-8 and in Epstein–Barr Virus (EBV) Transformed Human B Cells: Loss of IL-13 Receptors on Burkitt's Lymphoma B Cells

Gee

Kozlowski

Kryworuchko

et al. 2001

Cellular Immunology

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Differential effect of heat shock on RNA metabolism in human Burkitt's lymphoma B-cell lines

Cited by 4 publications

References 35 publications

Nuclear deadenylation/polyadenylation factors regulate 3′ processing in response to DNA damage

Nuclear deadenylation/polyadenylation factors regulate 3′ processing in response to DNA damage

Marked differences between avian and mammalian testicular cells in the heat shock induction and polyadenylation of Hsp70 and ubiquitin transcripts

Differential Effect of IL-4 and IL-13 on CD44 Expression in the Burkitt's Lymphoma B Cell Line BL30/B95-8 and in Epstein–Barr Virus (EBV) Transformed Human B Cells: Loss of IL-13 Receptors on Burkitt's Lymphoma B Cells

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