Marked differences between avian and mammalian testicular cells in the heat shock induction and polyadenylation of Hsp70 and ubiquitin transcripts

Mezquita, Belén; Mezquita, Cristóbal; Mezquita, Jovita

doi:10.1016/s0014-5793(98)01172-7

Cited by 30 publications

(18 citation statements)

References 31 publications

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“…Total RNA was isolated from bacteria from each of the c1, c2, and c3 infections and used to probe a P. multocida strain PM70 microarray that contained all 2,015 of the annotated P. multocida genes (32). Control RNA was isolated from P. multocida strain X-73 grown aerobically at 41°C (the normal body temperature of chickens [30]) in BHI to a cell density similar to that in the in vivo-grown samples (5 ϫ 10 9 CFU/ml). Microarrays were hybridized with control and experimental RNA labeled with either cy3 or cy5.…”

Section: Resultsmentioning

confidence: 99%

Genomic Scale Analysis of Pasteurella multocida Gene Expression during Growth within the Natural Chicken Host

et al. 2002

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Little is known about the genomic-scale transcriptional responses of bacteria during natural infections. We used whole-genome microarray analysis to assess the transcriptional state of the gram-negative pathogen Pasteurella multocida, the causative agent of fowl cholera, during infection in the natural chicken host. We compared the expression profiles of bacteria harvested from the blood of septicemic chickens experiencing late-stage fowl cholera with those from bacteria grown in rich medium. Independent analysis of bacterial expression profiles from the infection of three individual chickens indicated that 40 genes were differentially expressed in all three individuals, 126 were differentially expressed in two of the three individuals, and another 372 were differentially expressed in one individual. Real-time reverse transcription-PCR assays were used to confirm the expression ratios for a number of genes. Of the 40 genes differentially expressed in all three individuals, 17 were up-regulated and 23 were down-regulated in the host compared with those grown in rich medium. The majority (10 of 17) of the up-regulated genes were involved in amino acid transport and metabolism and energy production and conversion, clearly indicating how P. multocida alters its biosynthetic and energy production pathways to cope with the host environment. In contrast, the majority (15 of 23) of down-regulated genes were of unknown or poorly characterized functions. There were clear differences in gene expression between the bacteria isolated from each of the three chickens, a finding consistent with individual host variation being an important factor in determining pathogen gene expression. Interestingly, bacteria from only two of the three infected animals had a gene expression profile highly similar to that observed during growth under iron-limiting conditions, suggesting that severe iron starvation may not always occur during P. multocida infection.

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Section: Resultsmentioning

confidence: 99%

Genomic Scale Analysis of Pasteurella multocida Gene Expression during Growth within the Natural Chicken Host

et al. 2002

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“…transcript is its high degree of polyadenylation, not observed in any other tissue. We have previously reported the existence of high polyadenylated forms of ubiquitin, Hsp70 and GAPDH transcripts in adult chicken testis [Mezquita et al, 1998[Mezquita et al, , 1998b. In addition, we have reported that polyadenylation markedly increased upon heat shock during chicken spermatogenesis [Mezquita et al, 1998b].…”

Section: Discussionmentioning

confidence: 80%

“…We have previously reported the existence of high polyadenylated forms of ubiquitin, Hsp70 and GAPDH transcripts in adult chicken testis [Mezquita et al, 1998[Mezquita et al, , 1998b. In addition, we have reported that polyadenylation markedly increased upon heat shock during chicken spermatogenesis [Mezquita et al, 1998b]. It has been proposed that polyadenylation enhances the stability of the transcripts, the efficiency of translation, and the mRNA export from the nucleus [Kleene, 1996;Sachs et al, 1997;Huang and Carmichael, 1996].…”

Section: Discussionmentioning

confidence: 94%

Expression of lactate dehydrogenases A and B during chicken spermatogenesis: Characterization of testis specific transcripts

Arias

Mezquita

2000

J. Cell. Biochem.

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The substrates required for glycolysis change markedly at successive stages of spermatogenesis suggesting a considerable plasticity in the expression of glycolytic enzymes. Lactate dehydrogenase (LDH) isoenzymes, LDH-A and LDH-B, are expressed in premeiotic, meiotic cells, and early spermatids, both in avian and mammalian spermatogenesis. Highly polyadenylated forms, particularly of LDH-A, were detected in chicken testis. While mammals and columbid birds express the testis specific LDH-C gene in meiotic and postmeiotic cells, several LDH-B testis specific transcripts were detected in the corresponding cells during chicken spermatogenesis. These testis specific transcripts and the mRNA of mammalian LDH-C show several properties in common, such as temporal correlation of expression, mRNA stability, and repression of premature translation. These observations suggest that the testis specific transcripts could perform during chicken spermatogenesis the functions of the LDH-C mRNA in mammalian testis.

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“…The high temperature sensitivity during gametogenesis, especially spermatogenesis, is found across invertebrates 34 35 and vertebrates 36 , except birds 37 . Its biochemical mechanism is understood best among mammals.…”

Section: Published Datamentioning

confidence: 99%

How warm is too warm for the life cycle of actinopterygian fishes?

Motani

Wainwright

2015

Sci Rep

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We investigated the highest constant temperature at which actinopterygian fishes can complete their lifecycles, based on an oxygen supply model for cleavage-stage eggs. This stage is one of the most heat-sensitive periods during the lifecycle, likely reflecting the exhaustion of maternally supplied heat shock proteins without new production. The model suggests that average eggs would not develop normally under a constant temperature of about 36 °C or higher. This estimate matches published empirical values derived from laboratory and field observations. Spermatogenesis is more heat sensitive than embryogenesis in fishes, so the threshold may indeed be lower, at about 35 °C, unless actinopterygian fishes evolve heat tolerance during spermatogenesis as in birds. Our model also predicts an inverse relationship between egg size and temperature, and empirical data support this prediction. Therefore, the average egg size, and hence hatching size, is expected to shrink in a greenhouse world but a feeding function prohibits the survival of very small hatchlings, posing a limit to the shrinkage. It was once suggested that a marine animal community may be sustained under temperatures up to about 38 °C, and this value is being used, for example, in paleotemperature reconstruction. A revision of the value is overdue. (199/200)

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Marked differences between avian and mammalian testicular cells in the heat shock induction and polyadenylation of Hsp70 and ubiquitin transcripts

Cited by 30 publications

References 31 publications

Genomic Scale Analysis of Pasteurella multocida Gene Expression during Growth within the Natural Chicken Host

Genomic Scale Analysis of Pasteurella multocida Gene Expression during Growth within the Natural Chicken Host

Expression of lactate dehydrogenases A and B during chicken spermatogenesis: Characterization of testis specific transcripts

How warm is too warm for the life cycle of actinopterygian fishes?

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