Differential distribution of transcripts from genes involved in polyamine biosynthesis in bean plants

Abstract

Partial cDNAs sequences for arginine decarboxylase (Pvadc), S-adenosylmethionine decarboxylase (Pvsamdc) and spermidine synthase (Pvspds) were isolated from the bean Phaseolus vulgaris using primers designed from conserved regions of enzymes belonging to plant species. Sequence analysis showed that the Pvadc, Pvsamdc and Pvspds genes were most closely related to the orthologous genes from Glycine max, Phaseolus lunatus and Pisum sativum, respectively. The expression patterns of the genes, together with that of ornithine decarboxylase (Pvodc), were analysed in young and mature leaves, stems, roots, root tips, petals, stigma, ovaries, filaments and anthers of bean plants.Pvsamdc was found to be expressed at similar levels in all tissues. The other transcripts showed tissue specific expression. Pvadc was barely expressed in petals and not at all in roots tips, Pvspds was mainly expressed in roots, stigma and filaments, and Pvodc was detected only in roots.Additional key words: arginine decarboxylase, ornithine decarboxylase, Phaseolus vulgaris, S-adenosylmethionine decarboxylase, spermidine synthase.

Breaking seed dormancy in specially protected Turbinicarpus lophophoroides and Turbinicarpus pseudopectinatus (Cactaceae)

“…In general, polyamines have been implicated in various plant growth and developmental processes, such as stimulation of cell division, response to environmental stresses, regulation of rhizogenesis, somatic embryogenesis, pollen formation, floral development, initial fruit abscission, dormancy and senescence (Bouchereau et al . 1999; Jiménez‐Bremont et al . 2006a,b; Rodríguez‐Kessler et al .…”

Occurrence of polyamines in root nodules of Phaseolus vulgaris in symbiosis with Rhizobium tropici in response to salt stress

“…Finally, Spms expression was rather low in all organs tested which is consistent with the low levels of Spm detected in nodules. In general, these results indicate that the expression levels of the genes involved in the biosynthetic pathway of PAs are tissue-specific an do not follow a common pattern in different plant species which is probably the result of different regulation mechanism as stated by Jimenez-Bremont et al (2006).…”