Occurrence of polyamines in root nodules of Phaseolus vulgaris in symbiosis with Rhizobium tropici in response to salt stress

López‐Gómez, Miguel; Cobos-Porras, Libertad; Hidalgo-Castellanos, Javier; Lluch, Carmen

doi:10.1016/j.phytochem.2014.08.017

Cited by 22 publications

(9 citation statements)

References 43 publications

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“…PuuR in E. coli is known to bind putrescine, a polyamine (34, 35), which in cell plants has been implicated in protection against salt stress by stabilizing RNA, DNA, proteins, and phospholipids (35). Previous work in Phaseolus vulgaris determined that not only the number but also the variety of polyamines (including putrescine, spermidine, spermine, and homospermidine) found in nodules is higher compared to leaves and roots (35), suggesting a functional role in these structures. The regulation of PuuR over nodI , a nodulation gene, and nifU2 , a nitrogen fixation gene, suggests a role in both nodulation and nitrogen fixation ( Figure 8 ).…”

Section: Discussionmentioning

confidence: 99%

Putrescine acts as a signaling metabolite in the transition from nodulation to nitrogen fixation inRhizobium phaseoli

Hernandez-Benitez,

Martínez-Romero,

Aguirre-Noyola

et al. 2024

Preprint

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Growth of the common bean plant Phaseolus vulgaris is tightly linked to its symbiotic relationship with diverse rhizobial species, particularly Rhizobium phaseoli, an alphaproteobacteria that forms root nodules and provides high levels of nitrogen to the plant. Molecular cross-talk is known to happen via plant-derived metabolites, but only flavonoids have been identified as signals. Flavonoids are transported inside the bacteria, where they signal the NodD regulator to elicit nodulation. Although seven other regulators are known to be involved, our knowledge of the regulatory mechanisms underlying the nodulation, and nitrogen fixation processes is limited, and the signals recognized by regulators are mostly unknown. Here, we identified 75 transcription factors in R. phaseoli genome through sequence conservation from Escherichia coli, and assembled a transcriptional regulatory network comprising 24 regulators, and 652 target genes. We identified the interactions relevant to nodulation via gene expression, and signaled out putrescine as a signaling metabolite. We propose a model where putrescine acts as a switch on the transition from nodulation to nitrogen fixation via the dual transcription factor PuuR, and its regulation of the nodI and nifU2 genes.

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Section: Discussionmentioning

confidence: 99%

Putrescine acts as a signaling metabolite in the transition from nodulation to nitrogen fixation inRhizobium phaseoli

Hernandez-Benitez,

Martínez-Romero,

Aguirre-Noyola

et al. 2024

Preprint

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“…A significant number of additional works have analyzed the salt-induced changes in the PAs profiles of different legumes like soybean (Zhang et al, 2014), bean (López-Gómez et al, 2014aShevyakova et al, 2013;Talaat, 2015;Zapata et al, 2008), L. tenuis (Maiale et al, 2004;Sanchez et al, 2005;Sannazzaro et al, 2007). Taken together, these works do not show a congruent pattern of PAs changes due to saline treatment, not even within the same plant species,…”

Section: Salt Stressmentioning

confidence: 99%

Polyamines and legumes: Joint stories of stress, nitrogen fixation and environment

Menéndez

Calzadilla

Sansberro

et al. 2019

Preprint

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Polyamines (PAs) are natural aliphatic amines involved in many physiological processes in almost all living organisms, including responses to abiotic stresses and microbial interactions. This review presents the profuse evidence that relates changes in polyamines levels during responses to biotic and abiotic stresses in model and cultivable species within Leguminosae, and examines their potential roles in the functioning of symbiotic interactions with nitrogen fixing bacteria and arbuscular mycorrhizae. The family Leguminosae constitutes an economically and ecologically key botanical group for humans, being also regarded as the most important protein source for livestock. The ability of legumes to establish symbiotic interactions with nitrogen fixing bacteria and arbuscular mycorrhizae fungi, gives to some legume species that are able to exploit their molecular machinery “pioneer” attributes, with better competition in nutrient-poor soils and higher adaptation to restricted environments. However, many legume crops may be affected by climate change-derived environmental stresses, whereby maintaining their yields safe from adverse environmental conditions is probably one of the biggest challenge facing modern agriculture. Therefore, the obtaining of vigorous genotypes with higher tolerance to abiotic and biotic stressors has turned an increasingly important biotechnological target. At this scenario, PAs can play an important role, and genetic manipulation of crop plants with genes encoding polyamine PA biosynthetic pathway enzymes is envisioned as a strategy to achieve plants with improved stress tolerance and symbiotic performance. As linking plant physiological behavior with "big data" available in "omics" is an essential step to improve our understanding of legumes responses to global change, we also examined integrative MultiOmics approaches available to decrypt the interface legumes-PAs-abiotic and biotic stress or interactions. These approaches are expected to accelerate the identification of stress tolerant phenotypes and the design of new biotechnological strategies to increase their yield and adaptation to marginal environments, making a better use of available plant genetic resources.

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“…The action of ROS in the apoplast may involve a delicate balance between cleavage and cross-linking activities (Cosgrove, 1999) and several authors have reported that apoplastic ROS promote cellular elongation (Rodríguez et al, 2009;Schopfer, 2001 A significant number of additional works have analyzed the salt-induced changes in the PAs profiles of different legumes like soybean (Zhang et al, 2014), bean (López-Gómez et al, 2014aShevyakova et al, 2013;Talaat, 2015;Zapata et al, 2008), L. tenuis (Maiale et al, 2004;Sanchez et al, 2005;Sannazzaro et al, 2007). Taken together, these works do not show a congruent pattern of PAs changes due to saline treatment, not even within the same plant species, whereby it is not possible to extract a unique conclusion on PAs physiological role on plant tolerance to salinity.…”

Section: Salt Stressmentioning

confidence: 99%

Polyamines and legumes: Joint stories of stress, nitrogen fixation and environment

Menéndez

Calzadilla

Sansberro

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

Occurrence of polyamines in root nodules of Phaseolus vulgaris in symbiosis with Rhizobium tropici in response to salt stress

Cited by 22 publications

References 43 publications

Putrescine acts as a signaling metabolite in the transition from nodulation to nitrogen fixation inRhizobium phaseoli

Putrescine acts as a signaling metabolite in the transition from nodulation to nitrogen fixation inRhizobium phaseoli

Polyamines and legumes: Joint stories of stress, nitrogen fixation and environment

Polyamines and legumes: Joint stories of stress, nitrogen fixation and environment

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