Differential diagnosis of rinderpest and PPR using biotinylated cDNA probes

Pandey, K. C.; Bar-On, Tamir; Barrett, Tom

doi:10.1136/vr.131.9.199

Cited by 20 publications

(13 citation statements)

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“…RT-PCR, simple and aqueous phase ELISA (SNAP-ELISA) and nucleic acid hybridization have also been used for the detection and differentiation of PPRV [35,43,53,54,105,127]. RT-PCR and cDNA hybridization techniques are the two sensitive means to diagnose the PPRV infection, but are time consuming and cumbersome for routine diagnosis with a large sample size.…”

Section: Nucleic Acid Hybridizationmentioning

confidence: 99%

“…RT-PCR and cDNA hybridization techniques are the two sensitive means to diagnose the PPRV infection, but are time consuming and cumbersome for routine diagnosis with a large sample size. Nucleic acid hybridization is also suitable for providing diagnosis utilizing field materials, which are either collected from the putrefied or semi-putrefied carcasses or get putrefied during transit period [41,105]. Diallo et al [41].…”

Section: Nucleic Acid Hybridizationmentioning

confidence: 99%

“…Therefore, specific diagnosis of PPRV infection can be achieved by cDNA hybridization [41,105,127,149], mobility of N protein [40,149], neutralization test [28,116,149] and MAb-based ELISA [81,82,135,136], RT-PCR [7,53] and Real time RT-PCR [12,13,18]. A battery of serological tests and molecular assays are available to detect and identify PPRV antigen/nucleic acid and antibodies.…”

Section: Diagnosismentioning

confidence: 99%

“…Due to improved understanding of the viral genome and molecular biological techniques, nucleic acid-based specific and sensitive assays were also developed [41,43,105,128].…”

Section: Molecular Diagnostic Techniquesmentioning

confidence: 99%

“…Advent of cell culture and molecular biological techniques has allowed development of specific, rapid and sensitive diagnostics. In general, virus isolation, AGID/AGPT [104], CIE [86], nucleic acid hybridization [41,105], hemagglutination using piglet [160] or chicken [126] red blood cells, immuno histochemical detection [118], serum or virus neutralization test (VNT) and ELISA using PPRV-specific monoclonal antibodies (MAbs) are used for diagnosis of PPR.…”

Section: Diagnosismentioning

confidence: 99%

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Diagnosis and control of peste des petits ruminants: a comprehensive review

Balamurugan¹,

Hemadri²,

Gajendragad³

et al. 2013

VirusDis.

122

118

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Peste des petits ruminants (PPR) is an acute, highly contagious, world organization for animal health (OIE) notifiable and economically important transboundary viral disease of sheep and goats associated with high morbidity and mortality and caused by PPR virus. PPR is considered as one of the main constraints in augmenting the productivity of small ruminants in developing countries and particularly severely affects poor farmer's economy. The disease is clinically manifested by pyrexia, oculo-nasal discharges, necrotizing and erosive stomatitis, gastroenteritis, diarrhoea and bronchopneumonia. The disease can be diagnosed from its clinical signs, pathological lesions, and specific detection of virus antigen/antibodies/genome in the clinical samples by various serological tests and molecular assays. PPR is the one of the priority animal diseases whose control is considered important for poverty alleviation in enzootic countries. Availability of effective and safe live attenuated cell culture PPR vaccines and diagnostics have boosted the recently launched centrally sponsored control programme in India and also in other countries. This review article primarily focus on the current scenario of PPR diagnosis and its control programme with advancement of research areas that have taken place in the recent years with future perspectives.

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Section: Nucleic Acid Hybridizationmentioning

confidence: 99%

Section: Nucleic Acid Hybridizationmentioning

confidence: 99%

Section: Diagnosismentioning

confidence: 99%

“…Due to improved understanding of the viral genome and molecular biological techniques, nucleic acid-based specific and sensitive assays were also developed [41,43,105,128].…”

Section: Molecular Diagnostic Techniquesmentioning

confidence: 99%

Section: Diagnosismentioning

confidence: 99%

See 3 more Smart Citations

Diagnosis and control of peste des petits ruminants: a comprehensive review

Balamurugan¹,

Hemadri²,

Gajendragad³

et al. 2013

VirusDis.

122

118

View full text Add to dashboard Cite

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Peste des Petits Ruminants

Balamurugan

2017

Emerging and Re-Emerging Infectious Diseases of Livestock

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A sandwich-ELISA for the diagnosis of Peste des petits ruminants (PPR) infection in small ruminants using anti-nucleocapsid protein monoclonal antibody

et al. 2004

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A sandwich ELISA test using PPR specific monoclonal antibody (clone 4G6) to an epitope of nucleocapsid protein has been developed. The test uses polyclonal sera to capture the antigen from clinical samples (swabs and tissues). Captured antigens from clinical samples are detected using PPR specific monoclonal antibody. The test is specific to PPR as it failed to detect rinderpest vaccine virus (RBOK strain). Varieties of clinical samples originating from laboratory experiments (n = 231) and from field (n = 259) were employed to test the efficacy of sandwich-ELISA test. The test compared very well with an internationally accepted commercial Immune-capture ELISA kit, which uses biotinylated monoclonal antibody against the nucleocapsid protein. On a parallel testing using 490 clinical samples, 4G6 MAb based sandwich ELISA had an overall relative diagnostic specificity of 92.8% and diagnostic sensitivity of 88.9% compared to the commercial kit. The newly developed test is free from prozone phenomenon. PPR outbreaks from various parts of India have been confirmed using the test. Findings suggested that the newly developed ELISA is suitable for PPR diagnosis under field conditions.

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Differential diagnosis of rinderpest and PPR using biotinylated cDNA probes

Cited by 20 publications

References 0 publications

Diagnosis and control of peste des petits ruminants: a comprehensive review

Diagnosis and control of peste des petits ruminants: a comprehensive review

Peste des Petits Ruminants

A sandwich-ELISA for the diagnosis of Peste des petits ruminants (PPR) infection in small ruminants using anti-nucleocapsid protein monoclonal antibody

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