Differential Detection of the Tobamoviruses Tomato Mosaic Virus (ToMV) and Tomato Brown Rugose Fruit Virus (ToBRFV) Using CRISPR-Cas12a

Alon, Dan Mark; Hak, Hagit; Bornstein, Menachem; Pines, Gur; Spiegelman, Ziv

doi:10.3390/plants10061256

Cited by 40 publications

(39 citation statements)

References 57 publications

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“…Careful literature overview highlighted several in silico studies based on the crystal structure of TMV CP (PDB code 1ei7) [ 39 , 72 , 73 , 89 , 91 , 94 , 97 , 105 , 106 , 107 ]. Due to the high conservation of the tobamovirus CP sequences [ 108 ] in this study, we considered the TMV CP sequence as a template. Several TMV CP structures are available in the PDB [ 84 , 85 ], with a resolution spanning from 2.8 Å to 2.4 Å [ 59 , 69 ].…”

Section: Discussionmentioning

confidence: 99%

Autoxidation Products of the Methanolic Extract of the Leaves of Combretum micranthum Exert Antiviral Activity against Tomato Brown Rugose Fruit Virus (ToBRFV)

Iobbi

Lanteri²,

Minuto³

et al. 2022

Molecules

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Tomato brown rugose fruit virus (ToBRFV) is a new damaging plant virus of great interest from both an economical and research point of view. ToBRFV is transmitted by contact, remains infective for months, and to-date, no resistant cultivars have been developed. Due to the relevance of this virus, new effective, sustainable, and operator-safe antiviral agents are needed. Thus, 4-hydroxybenzoic acid was identified as the main product of the alkaline autoxidation at high temperature of the methanolic extract of the leaves of C. micranthum, known for antiviral activity. The autoxidized extract and 4-hydroxybenzoic acid were assayed in in vitro experiments, in combination with a mechanical inoculation test of tomato plants. Catechinic acid, a common product of rearrangement of catechins in hot alkaline solution, was also tested. Degradation of the viral particles, evidenced by the absence of detectable ToBRFV RNA and the loss of virus infectivity, as a possible consequence of disassembly of the virus coat protein (CP), were shown. Homology modeling was then applied to prepare the protein model of ToBRFV CP, and its structure was optimized. Molecular docking simulation showed the interactions of the two compounds, with the amino acid residues responsible for CP-CP interactions. Catechinic acid showed the best binding energy value in comparison with ribavirin, an anti-tobamovirus agent.

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Section: Discussionmentioning

confidence: 99%

Autoxidation Products of the Methanolic Extract of the Leaves of Combretum micranthum Exert Antiviral Activity against Tomato Brown Rugose Fruit Virus (ToBRFV)

Iobbi

Lanteri²,

Minuto³

et al. 2022

Molecules

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show abstract

“…In addition, antibodies for ToBRFV are produced and commercialized and are employed in serological tests; however, cross-reactivity with other tobamoviruses is a possibility. Finally, a new detection method based on CRISPR/Cas technology has been recently described, which has the potential for use in the laboratory or field [61,62]. For the management of this disease, distinct strategies must be considered, such as the use of pathogen-free seeds, avoiding reusing substrates, the early elimination and removal of infected plants, the elimination of potential weed hosts located either inside or near the production area, crop rotation when possible, and avoiding the rotation of workers among greenhouses.…”

Section: Tomato Brown Rugose Fruit Virusmentioning

confidence: 99%

Viruses of Economic Impact on Tomato Crops in Mexico: From Diagnosis to Management—A Review

García‐Estrada

Diaz‐Lara

Aguilar-Molina

et al. 2022

Viruses

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Tomato is the most economically important vegetable crop worldwide and the second most important for Mexico. However, viral diseases are among the main limiting factors that affect the productivity of this crop, causing total losses in some cases. This review provides key information and findings on the symptoms, distribution, transmission, detection, and management of diseases caused by viruses of major importance in tomato crops in Mexico. Currently, about 25 viruses belonging to nine different families have been reported infecting tomato in Mexico, but not all of them cause economically significant diseases. Viruses of economic importance include tomato brown rugose fruit virus (ToBRFV), tomato spotted wilt virus (TSWV), tomato yellow leaf curl virus (TYLCV), pepino mosaic virus (PepMV), and tomato marchitez virus (ToMarV). The topics discussed here will provide updated information about the status of these plant viruses in Mexico as well as diverse management strategies that can be implemented according to the specific circumstances of each viral pathosystem. Additionally, a list of tomato-affecting viruses not present in Mexico that are continuous threats to the crop health is included.

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“…The presence of PCR inhibitors in analyzed plant samples can also cause problems. Other, newer approaches to the detection of plant viruses are already being used, such as CRISPR/Cas12a-based detection [21] or high-throughput sequencing [22,23]. The latter has brought about an approach for virus identification that does not require knowledge about the virus infecting a given plant.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of New Polyclonal Antibody Developed for Serological Diagnostics of Tomato Mosaic Virus

Mrkvová

Hančinský

Grešíková

et al. 2022

Viruses

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Plant viruses threaten agricultural production by reducing the yield, quality, and economical benefits. Tomato mosaic virus (ToMV) from the genus Tobamovirus causes serious losses in the quantity and quality of tomato production. The management of plant protection is very difficult, mainly due to the vector-less transmission of ToMV. Resistant breeding generally has low effectiveness. The most practical approach is the use of a rapid diagnostic assay of the virus’ presence before the symptoms occur in plants, followed by the eradication of virus-infected plants. Such approaches also include serological detection methods (ELISA and Western immunoblotting), where antibodies need to be developed for an immunochemical reaction. The development and characterization of polyclonal antibodies for the detection of ToMV with appropriate parameters (sensitivity, specificity, and cross-reactivity) were the subjects of this study. A new polyclonal antibody, AB-1, was developed in immunized rabbits using the modified oligopeptides with antigenic potential (sequences are revealed) derived from the coat protein of ToMV SL-1. the developed polyclonal antibody. AB-1, showed higher sensitivity when compared with commercially available analogs. It also detected ToMV in infected pepper and eggplant plants, and detected another two tobamoviruses (TMV and PMMoV) and ToMV in soil rhizosphere samples and root residues, even two years after the cultivation of the infected tomato plant.

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Differential Detection of the Tobamoviruses Tomato Mosaic Virus (ToMV) and Tomato Brown Rugose Fruit Virus (ToBRFV) Using CRISPR-Cas12a

Cited by 40 publications

References 57 publications

Autoxidation Products of the Methanolic Extract of the Leaves of Combretum micranthum Exert Antiviral Activity against Tomato Brown Rugose Fruit Virus (ToBRFV)

Autoxidation Products of the Methanolic Extract of the Leaves of Combretum micranthum Exert Antiviral Activity against Tomato Brown Rugose Fruit Virus (ToBRFV)

Viruses of Economic Impact on Tomato Crops in Mexico: From Diagnosis to Management—A Review

Evaluation of New Polyclonal Antibody Developed for Serological Diagnostics of Tomato Mosaic Virus

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