Differential Cytotoxicity Induced by Transition Metal Oxide Nanoparticles is a Function of Cell Killing and Suppression of Cell Proliferation

Tolliver, Larry M.; Holl, Natalie J.; Hou, Fang; Lee, Han-Jung; Cambre, Melissa Hope; Huang, Yue-Wern

doi:10.3390/ijms21051731

Cited by 16 publications

(9 citation statements)

References 35 publications

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“…95 In addition, the S/G2 transition is a crucial regulatory step to determine the timing of mitosis, which based on the successful DNA synthesis. The induction of cell cycle arrest in S phase by mixed-form nano-TiO 2 indicated that the particles may directly damage DNA or influence DNA replication machinery, 96 and it can also be attributed to the S-phase arrest caused by anatase-form nano-TiO 2 that we observed in cancer cells, since the mixed-form nano-TiO 2 is formed by rutile and anatase. We speculated that the decrease in the percentage of G0/G1 phase cells caused by mixed-form nano-TiO 2 may be related to the induction of S-phase arrest.…”

Section: Discussionmentioning

confidence: 67%

Effect of Titanium Dioxide Nanoparticles on Mammalian Cell Cycle In Vitro: A Systematic Review and Meta-Analysis

Chang

Wang

Meng

et al. 2022

Chem. Res. Toxicol.

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Although most studies that explore the cytotoxicity of titanium dioxide nanoparticles (nano-TiO2) have focused on cell viability and oxidative stress, the cell cycle, a basic process of cell life, can also be affected. However, the results on the effects of nano-TiO2 on mammalian cell cycle are still inconsistent. A systematic review and meta-analysis were therefore performed in this research based on the effects of nano-TiO2 on the mammalian cell cycle in vitro to explore whether nano-TiO2 can induce cell cycle arrest. Meanwhile, the impact of physicochemical properties of nano-TiO2 on the cell cycle in vitro was investigated, and the response of normal cells and cancer cells was compared. A total of 33 articles met the eligibility criteria after screening. We used Review Manager 5.4 and Stata 15.1 for analysis. The results showed an increased percentage of cells in the sub-G1 phase and an upregulation of the p53 gene after being exposed to nano-TiO2. Nevertheless, nano-TiO2 had no effect on cell percentage in other phases of the cell cycle. Furthermore, subgroup analysis revealed that the cell percentage in both the sub-G1 phase of normal cells and S phase of cancer cells were significantly increased under anatase-form nano-TiO2 treatment. Moreover, nano-TiO2 with a particle size <25 nm or exposure duration of nano-TiO2 more than 24 h induced an increased percentage of normal cells in the sub-G1 phase. In addition, the cell cycle of cancer cells was arrested in the S phase no matter if the exposure duration of nano-TiO2 was more than 24 h or the exposure concentration was over 50 μg/mL. In conclusion, this study demonstrated that nano-TiO2 disrupted the cell cycle in vitro. The cell cycle arrest induced by nano-TiO2 varies with cell status and physicochemical properties of nano-TiO2.

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Section: Discussionmentioning

confidence: 67%

Effect of Titanium Dioxide Nanoparticles on Mammalian Cell Cycle In Vitro: A Systematic Review and Meta-Analysis

Chang

Wang

Meng

et al. 2022

Chem. Res. Toxicol.

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“…The included studies used differing methods in assessing cytotoxicity and genotoxicity: cell membrane integrity was assessed with Lactate dehydrogenase (LDH) assays [ 44 , 57 , 116 ]; cell viability was assessed using tetrazolium reduction assays [ 82 , 83 , 90 , 116 ]; apoptosis was assessed using immunohistochemistry biomarkers [ 60 , 65 , 86 ]; electron microscopy was used to assess intracellular localisation of nanoparticles [ 34 , 106 ]; and cell inflammation was estimated using chemokines biomarkers (i.e., IL-8, TNF- α, and IL-6) [ 146 ]. Compounds such as MTT, XTT, MTS, and WST-1 are used to detect viable cells [ 147 ].…”

Section: Discussionmentioning

confidence: 99%

Human and environmental impacts of nanoparticles: a scoping review of the current literature

et al. 2023

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Use of nanoparticles have established benefits in a wide range of applications, however, the effects of exposure to nanoparticles on health and the environmental risks associated with the production and use of nanoparticles are less well-established. The present study addresses this gap in knowledge by examining, through a scoping review of the current literature, the effects of nanoparticles on human health and the environment. We searched relevant databases including Medline, Web of Science, ScienceDirect, Scopus, CINAHL, Embase, and SAGE journals, as well as Google, Google Scholar, and grey literature from June 2021 to July 2021. After removing duplicate articles, the title and abstracts of 1495 articles were first screened followed by the full-texts of 249 studies, and this resulted in the inclusion of 117 studies in the presented review.In this contribution we conclude that while nanoparticles offer distinct benefits in a range of applications, they pose significant threats to humans and the environment. Using several biological models and biomarkers, the included studies revealed the toxic effects of nanoparticles (mainly zinc oxide, silicon dioxide, titanium dioxide, silver, and carbon nanotubes) to include cell death, production of oxidative stress, DNA damage, apoptosis, and induction of inflammatory responses. Most of the included studies (65.81%) investigated inorganic-based nanoparticles. In terms of biomarkers, most studies (76.9%) used immortalised cell lines, whiles 18.8% used primary cells as the biomarker for assessing human health effect of nanoparticles. Biomarkers that were used for assessing environmental impact of nanoparticles included soil samples and soybean seeds, zebrafish larvae, fish, and Daphnia magna neonates.From the studies included in this work the United States recorded the highest number of publications (n = 30, 25.64%), followed by China, India, and Saudi Arabia recording the same number of publications (n = 8 each), with 95.75% of the studies published from the year 2009. The majority of the included studies (93.16%) assessed impact of nanoparticles on human health, and 95.7% used experimental study design. This shows a clear gap exists in examining the impact of nanoparticles on the environment.

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“…For cytotoxicity assay using SRB dye (Tolliver et al, 2020); cells were seeded in each well of 96-well plate at a seeding density of 0.5*10 4 per well in a 100 µL culture medium. The plate was then incubated for 24 hours at 37 °C in a 5 % CO2 incubator.…”

Section: ) Cell Line and Cytotoxicity Studymentioning

confidence: 99%

Titanium Dioxide and Its Effect on Human Health and Environment- An in vitro Study

Utgikar

Kadam²,

Rambhiya³

et al. 2022

EJBIO

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This work focuses on understanding the in-vitro effects of Titanium Dioxide (TiO2) led in the environment on human health, algae, and agriculture. Human dermal fibroblasts (HDF) from juvenile foreskin and human red blood cells (HRBC) were taken as a model to study the effect of TiO2 on human health in vitro. The study intended to define the toxic effect of TiO2 at 50 ppm, 100 ppm, and 200 ppm. The effect of TiO2 on the algae cyanobacterium as a model to analyze the algal toxicity effect i.e. was checked for freshwater toxicity at the concentration of TiO2 at 100 and 200 ppm was studied. The effect of TiO2 on the growth of microorganisms in wastewater was studied to determine its biodegradation in-vitro for 5 days using dissolved oxygen determination and the biological oxidation demand (BOD). Finally, phytotoxicity was monitored by observing the effect of TiO2 on wheat seed germination. It was found that TiO2 had no effect on HDF and HRBC at the tested concentrations as no cell death and hemolysis were observed when the cells were treated with TiO2. However, a statistically significant algal toxicity of 32.14 % was observed at 100 ppm and a 42.86 % (p<0.01) decrease in biomass was observed at 200 ppm. Additionally, there was no effect found on BOD of wastewater in the presence or absence of TiO2. The TiO2 had a positive effect on wheat seed germination in a dose-dependent manner. There was an increase in root length from 3.4 cm to 4.3 cm and 4.6 cm at 100 and 200 ppm of TiO2, respectively. Also, a slight increase in shoot length was observed at 100 ppm and 200 ppm. However, visible root thinning was a drawback observed. Hence, the present study gives an elaborative insight into the effects of Titanium Dioxide on human health and the environment.

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Differential Cytotoxicity Induced by Transition Metal Oxide Nanoparticles is a Function of Cell Killing and Suppression of Cell Proliferation

Cited by 16 publications

References 35 publications

Effect of Titanium Dioxide Nanoparticles on Mammalian Cell Cycle In Vitro: A Systematic Review and Meta-Analysis

Effect of Titanium Dioxide Nanoparticles on Mammalian Cell Cycle In Vitro: A Systematic Review and Meta-Analysis

Human and environmental impacts of nanoparticles: a scoping review of the current literature

Titanium Dioxide and Its Effect on Human Health and Environment- An in vitro Study

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