Differential crosstalk between global DNA methylation and metabolomics associated with cell type specific stress response by pristine and functionalized MWCNT

Chatterjee, Nivedita; Yang, Jaesung; Yoon, Dahye; Kim, Suhkmann; Joo, Sang‐Woo; Choi, Jinhee

doi:10.1016/j.biomaterials.2016.11.005

Cited by 32 publications

(31 citation statements)

References 48 publications

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“…These techniques have been widely applied to identify biomarkers, characterize complex biochemical systems, study pathophysiological processes, map mechanisms of action and discover targets of novel drugs 14 – 18 . The cancer metabolome, as the complete set of small-molecule chemicals found within a biological sample, could be an important source for the discovery of molecular targets and mechanisms of action 19 , 20 . As a major carrier and functional executor of cellular activities, proteins own more biological information as compared to metabolites 21 .…”

Section: Introductionmentioning

confidence: 99%

8u, a pro-apoptosis/cell cycle arrest compound, suppresses invasion and metastasis through HSP90α downregulating and PI3K/Akt inactivation in hepatocellular carcinoma cells

Wang

Chen

Zhang

et al. 2018

Sci Rep

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8u, an acridine derivative, has been proved effective anti-hepatocarcinoma effect, while the underlying mechanism remains unclear. Here, metabolomics and proteomics approaches were applied to study its anti-cancer mechanism and explore its effect on HepG2 cells’ invasion and metastasis abilities. The results showed that 8u significantly suppressed HepG2 cells migration and enhanced cell-to-cell junctions. The inhibition effect of 8u on invasion and metastasis disappeared after HSP90α gene silencing, and was reversed after HSP90α overexpression. The biological experimental results indicated that 8u also blocked PI3K/Akt pathway, thereby reducing fatty acid synthase (FASN) protein expression and disordering intracellular lipid metabolism to inhibit cell invasion and metastasis. In addition, HSP90α protein and PI3K/Akt pathway could co-adjust to each other. These findings demonstrated that 8u could efficiently suppress the invasion and metastasis of HepG2 cells by decreasing the expression of HSP90α protein and inhibiting the PI3K/Akt signaling pathway, which could be used as a potential candidate for the treatment of HCC.

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Section: Introductionmentioning

confidence: 99%

8u, a pro-apoptosis/cell cycle arrest compound, suppresses invasion and metastasis through HSP90α downregulating and PI3K/Akt inactivation in hepatocellular carcinoma cells

Wang

Chen

Zhang

et al. 2018

Sci Rep

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“…DNA methylation is highly cell type specific (55, 56). Thus, it is possible that the changes in DNA methylation that we observed are due to a change in cell‐type composition in the CRS hearts rather than an alteration in DNA methylation in cardiomyocytes (57).…”

Section: Discussionmentioning

confidence: 99%

Contribution of DNA methylation in chronic stress‐induced cardiac remodeling and arrhythmias in mice

Zhang¹,

Li²,

Liu³

et al. 2019

The FASEB Journal

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It is recognized that stress can induce cardiac dysfunction, but the underlying mechanisms are not well understood. The present study aimed to test the hypothesis that chronic negative stress leads to alterations in DNA methylation of certain cardiac genes, which in turn contribute to pathologic remodeling of the heart. We found that mice that were exposed to chronic restraint stress (CRS) for 4 wk exhibited cardiac remodeling toward heart failure, as characterized by ventricular chamber dilatation, wall thinning, and decreased contractility. CRS also induced cardiac arrhythmias, including intermittent sinus tachycardia and bradycardia, frequent premature ventricular contraction, and sporadic atrioventricular conduction block. Circulating levels of stress hormones were elevated, and the cardiac expression of tyrosine hydroxylase, a marker of sympathetic innervation, was increased in CRS mice. Using reduced representation bisulfite sequencing, we found that although CRS did not lead to global changes in DNA methylation in the murine heart, it nevertheless altered methylation at specific genes that are associated with the dilated cardiomyopathy (DCM) (e.g., desmin) and adrenergic signaling of cardiomyocytes (ASPC) (e.g., adrenergic receptor‐α1) pathways. We conclude that CRS induces cardiac remodeling and arrhythmias, potentially through altered methylation of myocardial genes associated with the DCM and ASPC pathways.—Zhang P., Li, T., Liu, Y.‐Q., Zhang, H., Xue, S.‐M., Li, G., Cheng, H.‐Y.M., Cao, J.‐M. Contribution of DNA methylation in chronic stress‐induced cardiac remodeling and arrhythmias in mice. FASEB J. 33, 12240‐12252 (2019). http://www.fasebj.org

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“…This information may be important to define the hazardous properties of such nanoscale materials, a critical step in the identification of suitable biomarkers. Different ENMs, including silver (Ag)- (Müller et al, 2018; Murphy et al, 2016), titanium dioxide (TiO 2 )- (Kongseng et al, 2016; Patil et al, 2016; Renwick et al, 2001), zinc oxide (ZnO)- (Patil et al, 2016), silica (SiO 2 )- (Breznan et al, 2017), magnesium oxide (MgO)-NPs (Mahmoud et al, 2016), and carbon based-NMs (Chatterjee et al, 2017; Chortarea et al, 2017; Kim and Yu, 2014), demonstrated cyto-toxic, inflammatory, and oxidative stress effects, as well as the reduction of specific cellular function in vitro models. The small ENM size, resulting in a high surface area to volume ratio, may enhance ENM biological reactivity and relative toxicity (Guichard et al, 2016; Kim and Ryu, 2013; Soares et al, 2016; Uboldi et al, 2016).…”

Section: Hazard Identificationmentioning

confidence: 99%

Biological monitoring of workers exposed to engineered nanomaterials

et al. 2018

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As the number of nanomaterial workers increase there is need to consider whether biomonitoring of exposure should be used as a routine risk management tool. Currently, no biomonitoring of nanomaterials is mandated by authoritative or regulatory agencies. However, there is a growing knowledge base to support such biomonitoring, but further research is needed as are investigations of priorities for biomonitoring. That research should be focused on validation of biomarkers of exposure and effect. Some biomarkers of effect are generally nonspecific. These biomarkers need further interpretation before they should be used. Overall biomonitoring of nanomaterial workers may be important to supplement risk assessment and risk management efforts.

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Differential crosstalk between global DNA methylation and metabolomics associated with cell type specific stress response by pristine and functionalized MWCNT

Cited by 32 publications

References 48 publications

8u, a pro-apoptosis/cell cycle arrest compound, suppresses invasion and metastasis through HSP90α downregulating and PI3K/Akt inactivation in hepatocellular carcinoma cells

8u, a pro-apoptosis/cell cycle arrest compound, suppresses invasion and metastasis through HSP90α downregulating and PI3K/Akt inactivation in hepatocellular carcinoma cells

Contribution of DNA methylation in chronic stress‐induced cardiac remodeling and arrhythmias in mice

Biological monitoring of workers exposed to engineered nanomaterials

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