Differential changes in antioxidants, proteases, and lipid peroxidation in flag leaves of wheat genotypes under different levels of water deficit conditions

Hameed, Amjad; Bibi, Nizakat; Akhter, Javed; Iqbal, Nayyer

doi:10.1016/j.plaphy.2010.11.009

Cited by 116 publications

(63 citation statements)

References 30 publications

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“…It may be reflected more efficient role antioxidant enzymes in compare to non-enzymatic in protects cell systems against oxidative damage. These results are similar to works of Amjad et al (2011) and Shao et al (2005). H 2 O 2 and MSI had the highest correlation with YSI in all the traits.…”

Section: Pearsosupporting

confidence: 90%

Influence of Drought Stress on Oxidative Damage and Antioxidant Defense Systems in Tolerant and Susceptible Wheat Genotypes

Hasheminasab¹,

Assad²,

Aliakbari³

et al. 2012

JAS

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Drought is one of the major factors limiting crop production in arid and semi-arid regions. Twenty wheat genotypes with wide range of sensitivity to drought, including 18 varieties of bread wheat (Triticum aestivum L.) and two varieties of durum wheat (Triticum turgidum L.) were used in two separate field experiments in 2009-2010 at the Experimental Station of College of Agriculture in Shiraz University. Each experiment was conducted as a randomized completed block design with three replications. The moisture level in one of the experiments was optimum (100% field capacity) while the second experiment was conducted under drought stress (45% field capacity). Several biochemical components including enzymatic (catalase, CAT; peroxidase, POD; superoxide dismutase, SOD and ascorbate peroxidase, APX) and non-enzymatic (proline and carotenoids, Car) antioxidant defense systems and some factors of oxidative damage (hydrogen peroxide, H 2 O 2 ; lipid peroxidation, LPO and membrane stability index, MSI) were analyzed in the two conditions. Drought stress caused significant increase in enzymatic antioxidant activities, proline content, H 2 O 2 and LPO content at the flowering stage, while Car content and MSI decreased significantly in all genotypes. Drought tolerant genotypes showed the highest enzymatic and non-enzymatic antioxidants, highest MSI and the lowest LPO and H 2 O 2 . This trend was reversed in susceptible genotypes. The enzymatic antioxidants had higher correlation than non-enzymatic with oxidative stress factors and yield stability index (YSI). POD showed the highest positive correlation with MSI and the highest negative correlation with LPO. H 2 O 2 and MSI showed the highest correlation with YSI. In present study, Kavir and Alamut varieties were selected respectively as the most tolerant and susceptible genotypes.

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Section: Pearsosupporting

confidence: 90%

Influence of Drought Stress on Oxidative Damage and Antioxidant Defense Systems in Tolerant and Susceptible Wheat Genotypes

Hasheminasab¹,

Assad²,

Aliakbari³

et al. 2012

JAS

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“…3B). The higher activity of acidic proteolysis, induced by water deprivation and associated with a distinct reduction of protein content, is a common response of sensitive plants to dehydration (Cruz de Carvalho et al 2001;Hieng et al 2004;Simova-Stoilova et al 2010;Hameed et al 2011). As previously reported, (Wiśniewski and Zagdańska 2001;Grudkowska and Zagdańska 2010;Simova-Stoilova et al 2010) cysteine proteinases were the most active proteinases during a water deficiency.…”

Section: Discussionsupporting

confidence: 64%

“…This result was also true for frost-sensitive wheat seedlings (Grudkowska and Zagdańska 2010) and drought-sensitive wheat genotypes (Hameed et al 2011). An increase in protein carbonyl groups and oxidized protein thiol groups, with a concomitant significant repression of proteolytic activity, is commonly associated with cellular senescence (Smakowska et al 2014).…”

Section: Discussionsupporting

confidence: 61%

Changes in the reduction state of ascorbate and glutathione, protein oxidation and hydrolysis leading to the development of dehydration intolerance in Triticum aestivum L. seedlings

2015

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The present work demonstrates that the transition of wheat (Triticum aestivum L.) seedlings from dehydration tolerance to intolerance on the fifth day following imbibition is associated with a disturbance in cellular redox homeostasis. During germination the ratio of reduced (AsA) to oxidized ascorbate (DHA) was lower in the tolerant 4-day-old seedlings compared to the sensitive 6-day-old seedlings because of the lower ascorbate content in the former. The reduced glutathione (GSH) and total glutathione (GSH ? GSSG) pools were higher in tolerant seedlings and remained higher upon dehydration. The development of dehydration intolerance with a seedling age coincided with a 50 % loss of the total glutathione pool and a shift of GSH/GSSG to a more oxidized state. Activities of ascorbate peroxidase and glutathione reductase increased with water deficiency in both tolerant and sensitive seedlings but the three new activity bands appeared only in sensitive seedlings. The stable ratio of GSH/GSSG and a higher AsA/DHA ratio in sensitive seedlings did not prevent the enhanced production of H2O2 and the peroxidation of lipids with dehydration. As a result, an increase in the protein carbonyl group and a significant decrease in the thiol groups were observed in dehydrated sensitive seedlings. Water deficiency enhanced the total azocaseinolytic activity, mainly in sensitive seedlings. The highest increase in protein carbonylation and lowest azocaseinolytic activity was observed at the beginning of seedling dehydration (15-25 % WSD) being in a sensitive phase of growth. The presented results indicate that the development of dehydration intolerance during the transition of wheat seedlings from heterotrophic to autotrophic growth is associated with an alterations in protein oxidation.

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“…Reduction in absorbance of the reaction solution at 240 nm was recorded after every 20 s. An absorbance change of 0.01 unit's min −1 was defined as one unit CAT activity. On fresh weight basis, enzyme activity was expressed (Hameed et al 2011). …”

Section: Catalase (Cat)mentioning

confidence: 99%

Biochemical alterations in leaves of resistant and susceptible cotton genotypes infected systemically by cotton leaf curl Burewala virus

Siddique

Akhtar

Hameed

et al. 2014

Journal of Plant Interactions

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Leaf curl disease caused by Cotton Leaf Curl Burewala virus (CLCuBuV) has been recognized as serious threat to cotton in Indian subcontinent. However, information about cotton-CLCuBuV interaction is still limited. In this study, the level of phenolic compounds, total soluble proteins, and malondialdehyde (MDA) and the activities of phenylalanine ammonia-lyase (PAL), peroxidase (POX), catalase (CAT), proteases, superoxide dismutase (SOD), and polyphenol oxidase (PPO) were studied in leaves of two susceptible (CIM-496 & NIAB-111) and two resistant (Ravi and Co Tiep Khac) cotton genotypes. Disease symptoms were mild in the resistant genotypes but were severe in highly susceptible genotypes. The results showed that phenolic compounds, proteins, PAL, POX, CAT, proteases, SOD, PPO, and MDA play an active role in disease resistance against CLCuBuV. The amount of total phenols, proteases, MDA, and PPO was significantly higher in leaves of CLCuBuV-inoculated plants of both resistant genotypes as in non-inoculated plants, and decreased in CLCuBuV-inoculated plants of both susceptible genotypes over their healthy plants. POX, protein content, SOD, and PAL activities showed lower values in resistant genotypes, while they decreased significantly in susceptible genotypes as compared to the noninoculated plants except PAL, which showed non-significant decrease. CAT was found to be increased in both susceptible and resistant genotypes with maximum percent increase in resistant genotype Ravi, as compared to non-inoculated plants. The results showed significantly higher concentrations of total phenols and higher activity of protease, MDA, SOD, and PPO in resistant genotype Ravi after infection with CLCuBuV, suggesting that there is a correlation between constitutive induced levels of these enzymes and plant resistance that could be considered as biochemical markers for studying plant-virus compatible and incompatible interactions.

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Differential changes in antioxidants, proteases, and lipid peroxidation in flag leaves of wheat genotypes under different levels of water deficit conditions

Cited by 116 publications

References 30 publications

Influence of Drought Stress on Oxidative Damage and Antioxidant Defense Systems in Tolerant and Susceptible Wheat Genotypes

Influence of Drought Stress on Oxidative Damage and Antioxidant Defense Systems in Tolerant and Susceptible Wheat Genotypes

Changes in the reduction state of ascorbate and glutathione, protein oxidation and hydrolysis leading to the development of dehydration intolerance in Triticum aestivum L. seedlings

Biochemical alterations in leaves of resistant and susceptible cotton genotypes infected systemically by cotton leaf curl Burewala virus

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