Differential behavioral profiling of stimulant substances in the rat using the LABORAS™ system

Castagné, Vincent; Wolinsky, Toni D.; Quinn, Leann P.; Virley, David

doi:10.1016/j.pbb.2012.03.001

Cited by 16 publications

(6 citation statements)

References 30 publications

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“…In particular, we evaluated horizontal, vertical activity and distance travelled in standard motor activity cages, while in the other two studies, motor activity was quantified as number of crossings between any of the eight sections within the behavioural pattern monitor and by a descriptive statistic (spatial d) that calculated the spatial structure of locomotor paths (Halberstadt et al, ), or by trajectory length in cm (corrected for deviations less than 3 cm) over 5 min time blocks during 30 min sessions (Horsley et al, ). Remarkably, irrespective of the specific motor parameter affected by MXE and of the direction of its effects, all of the three studies indicated a biphasic effect of MXE on locomotor activity, as previously reported for ketamine and MK‐801 (Castagné et al, ).…”

Section: Discussionsupporting

confidence: 82%

Methoxetamine affects brain processing involved in emotional response in rats

Zanda

Fadda

Antinori

et al. 2017

British J Pharmacology

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BACKGROUND AND PURPOSEMethoxetamine (MXE) is a novel psychoactive substance that is emerging on the Internet and induces dissociative effects and acute toxicity. Its pharmacological effects have not yet been adequately investigated. EXPERIMENTAL APPROACHWe examined a range of behavioural effects induced by acute administration of MXE (0.5-5 mg·kg À1 ; i.p.) in rats and whether it causes rapid neuroadaptive molecular changes. KEY RESULTS MXE (0.5-5 mg·kgÀ1 ) affected motor activity in a dose-and time-dependent manner, inducing hypermotility and hypomotility at low and high doses respectively. At low and intermediate doses (0.5 and 1 mg·kg À1 ), MXE induced anxious and/or obsessive-compulsive traits (marble burying test), did not significantly increase sociability (social interaction test) or induce spatial anxiety (elevated plus maze test). At a high dose (5 mg·kg À1 ), MXE induced transient analgesia (tail-flick and hot-plate test), decreased social interaction time (social interaction test) and reduced immobility time while increasing swimming activity (forced swim test), suggesting an antidepressant effect. Acute MXE administration did not affect self-grooming behaviour at any dose tested. Immunohistochemical analysis showed that behaviourally active doses of MXE (1 and 5 mg·kg À1 ) increased phosphorylation of ribosomal protein S6 in the medial prefrontal cortex and hippocampus. CONCLUSIONS AND IMPLICATIONSMXE differentially affected motor activity, behaviour and emotional states in rats, depending on the dose tested. As reported for ketamine, phosphorylation of the ribosomal protein S6 was increased in MXE-treated animals, thus providing a 'molecular snapshot' of rapid neuroadaptive molecular changes induced by behaviourally active doses of MXE. Abbreviations

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Section: Discussionsupporting

confidence: 82%

Methoxetamine affects brain processing involved in emotional response in rats

Zanda

Fadda

Antinori

et al. 2017

British J Pharmacology

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“…Ambulatory activity measurements can be automated using photobeam arrays [ 15 ; 16 ; 17 ; 18 ; 19 ; 20 ], a passive infrared sensor [ 21 ; 22 ], a stabilimeter (‘rocking cage’/’jiggle box’) [ 15 ; 23 ] or birds-eye videotracking [ 24 ; 25 ; 26 ; 27 ]. A wider set of behaviors can be detected using pressure sensors beneath the cage [ 28 ; 29 ; 30 ] or by videoanalysis software [ 31 ]. These automated methods necessitate the rats, which are social animals [ 32 ; 33 ], to be singly housed, typically in bespoke cages, and generally on bench tops rather than cage racks, requiring dedicated space.…”

Section: Introductionmentioning

confidence: 99%

Automated recording of home cage activity and temperature of individual rats housed in social groups: The Rodent Big Brother project

et al. 2017

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Measuring the activity and temperature of rats is commonly required in biomedical research. Conventional approaches necessitate single housing, which affects their behavior and wellbeing. We have used a subcutaneous radiofrequency identification (RFID) transponder to measure ambulatory activity and temperature of individual rats when group-housed in conventional, rack-mounted home cages. The transponder location and temperature is detected by a matrix of antennae in a baseplate under the cage. An infrared high-definition camera acquires side-view video of the cage and also enables automated detection of vertical activity. Validation studies showed that baseplate-derived ambulatory activity correlated well with manual tracking and with side-view whole-cage video pixel movement. This technology enables individual behavioral and temperature data to be acquired continuously from group-housed rats in their familiar, home cage environment. We demonstrate its ability to reliably detect naturally occurring behavioral effects, extending beyond the capabilities of routine observational tests and conventional monitoring equipment. It has numerous potential applications including safety pharmacology, toxicology, circadian biology, disease models and drug discovery.

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“…In light of the antinociceptive synergism between curcumin and metformin in the formalin test, we evaluated the effects of individual compounds and their combination on motor coordination and short-term locomotor activity in naïve male-ICR mice. Measurement of locomotor activity allows us to determine whether the combination has potential side effects, either sedative or CNS stimulative 60 , 61 . The compounds in monotherapy or combination therapy were tested at the highest doses used in the formalin test (Fig.…”

Section: Resultsmentioning

confidence: 99%

Curcumin and metformin synergistically modulate peripheral and central immune mechanisms of pain

Wasana

Hasriadi

Muangnoi

et al. 2022

Sci Rep

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Metformin is a well-tolerated antidiabetic drug and has recently been repurposed for numerous diseases, including pain. However, a higher dose of metformin is required for effective analgesia, which can potentiate its dose-dependent gastrointestinal side effects. Curcumin is a natural polyphenol and has beneficial therapeutic effects on pain. Curcumin has been used as an analgesic adjuvant with several analgesic drugs, allowing synergistic antinociceptive effects. Nevertheless, whether curcumin can exert synergistic analgesia with metformin is still unknown. In the present study, the nature of curcumin-metformin anti-inflammatory interaction was evaluated in in vitro using lipopolysaccharide-induced RAW 264.7 macrophage and BV-2 microglia cells. In both macrophage and microglia, curcumin effectively potentiates the anti-inflammatory effects of metformin, indicating potential synergistic effects in both peripheral and central pathways of pain. The nature of the interaction between curcumin and metformin was further recapitulated using a mouse model of formalin-induced pain. Coadministration of curcumin and metformin at a 1:1 fixed ratio of their ED50 doses significantly reduced the dose required to produce a 50% effect compared to the theoretically required dose in phase II of the formalin test with a combination index value of 0.24. Besides, the synergistic interaction does not appear to involve severe CNS side effects indicated by no motor alterations, no alterations in short-term and long-term locomotive behaviors, and the general well-being of mice. Our findings suggest that curcumin exerts synergistic anti-inflammation with metformin with no potential CNS adverse effects.

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Differential behavioral profiling of stimulant substances in the rat using the LABORAS™ system

Cited by 16 publications

References 30 publications

Methoxetamine affects brain processing involved in emotional response in rats

Methoxetamine affects brain processing involved in emotional response in rats

Automated recording of home cage activity and temperature of individual rats housed in social groups: The Rodent Big Brother project

Curcumin and metformin synergistically modulate peripheral and central immune mechanisms of pain

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