Differential antioxidant defense and detoxification mechanisms in photodynamically stressed rice plants treated with the deregulators of porphyrin biosynthesis, 5-aminolevulinic acid and oxyfluorfen

Phung, Thu-Ha; Jung, Sunyo

doi:10.1016/j.bbrc.2015.02.125

Cited by 15 publications

(10 citation statements)

References 33 publications

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“…Therefore, a controlled flow of metabolites in the porphyrin biosynthetic pathway is essential to avoid photodynamic damage in stressed plants. Plants suffer severe photodynamic damage if these control mechanisms are circumvented, for example in plants treated with porphyrin deregulators including ALA and peroxidizing herbicides [ 29 , 30 ], plants with deregulation of porphyrin biosynthetic genes [ 31 , 32 ] or plants under environmental stress conditions [ 10 , 13 ]. The differential oxidative stress responses in OF- and MV-treated plants appear to be partly due to different perturbation of porphyrin metabolism, i.e.…”

Section: Resultsmentioning

confidence: 99%

Differential Antioxidant Responses and Perturbed Porphyrin Biosynthesis after Exposure to Oxyfluorfen and Methyl Viologen in Oryza sativa

Pham

Kim

Jung

2015

IJMS

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We compared antioxidant responses and regulation of porphyrin metabolism in rice plants treated with oxyfluorfen (OF) or methyl viologen (MV). Plants treated with MV exhibited not only greater increases in conductivity and malondialdehyde but also a greater decline in Fv/Fm, compared to plants treated with OF. MV-treated plants had greater increases in activities of superoxide dismutase (SOD) and catalase (CAT) as well as transcript levels of SODA and CATA than OF-treated plants after 28 h of the treatments, whereas increases in ascorbate peroxidase (APX) activity and transcript levels of APXA and APXB were greater in OF-treated plants. Both OF- and MV-treated plants resulted in not only down-regulation of most genes involved in porphyrin biosynthesis but also disappearance of Mg-porphyrins during the late stage of photooxidative stress. By contrast, up-regulation of heme oxygenase 2 (HO2) is possibly part of an efficient antioxidant response to compensate photooxidative damage in both treatments. Our data show that down-regulated biosynthesis and degradation dynamics of porphyrin intermediates have important roles in photoprotection of plants from perturbed porphyrin biosynthesis and photosynthetic electron transport. This study suggests that porphyrin scavenging as well as strong antioxidative activities are required for mitigating reactive oxygen species (ROS) production under photooxidative stress caused by OF and MV.

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Section: Resultsmentioning

confidence: 99%

Differential Antioxidant Responses and Perturbed Porphyrin Biosynthesis after Exposure to Oxyfluorfen and Methyl Viologen in Oryza sativa

Pham

Kim

Jung

2015

IJMS

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“…In cucumber seedlings, significantly enhanced activities of SOD, POD, CAT, APX (Ascorbate peroxidase), and GR (Glutathione reductase), and reduced ROS and MDA accumulation, are observed under ALA treatment combined with low-temperature stress [10]. Previous studies have reported that ALA activates the plant defense system and defense-related genes, such as genes encoding SOD, POD, CAT, and APX, in rice and strawberry under osmotic and photodynamic stresses and reduce overproduction of ROS and MDA [31][32][33]. ALA is a precursor of heme biosynthesis, and CAT, POD, and APX contain a heme prosthetic group [14], which might be the reason that antioxidant enzyme activities were stimulated in ALA-treated seedlings ( Figure 5).…”

Section: Discussionmentioning

confidence: 99%

Substrate Application of 5-Aminolevulinic Acid Enhanced Low-temperature and Weak-light Stress Tolerance in Cucumber (Cucumis sativus L.)

Anwar

Wang

et al. 2020

Agronomy

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5-Aminolevulinic acid (ALA) is a type of nonprotein amino acid that promotes plant stress tolerance. However, the underlying physiological and biochemical mechanisms are not fully understood. We investigated the role of ALA in low-temperature and weak-light stress tolerance in cucumber seedlings. Seedlings grown in different ALA treatments (0, 10, 20, or 30 mg ALA·kg−1 added to substrate) were exposed to low temperature (16/8 ˚C light/dark) and weak light (180 μmol·m−2·s−1 photosynthetically active radiation) for two weeks. Treatment with ALA significantly alleviated the inhibition of plant growth, and enhanced leaf area, and fresh and dry weight of the seedlings under low-temperature and weak-light stress. Moreover, ALA increased chlorophyll (Chl) a, Chl b, and Chl a+b contents. Net photosynthesis rate, stomatal conductance, transpiration rate, photochemical quenching, non-photochemical quenching, actual photochemical efficiency of photosystem II, and electron transport rate were significantly increased in ALA-treated seedlings. In addition, ALA increased root activity and antioxidant enzyme (superoxide dismutase, peroxidase, and catalase) activities, and reduced reactive oxygen species (hydrogen peroxide and superoxide radical) and malondialdehyde accumulation in the root and leaf of cucumber seedlings. These findings suggested that ALA incorporation in the substrate alleviated the adverse effects of low-temperature and weak-light stress, and improved Chl contents, photosynthetic capacity, and antioxidant enzyme activities, and thus enhanced cucumber seedling growth.

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“…that showed rapid membrane damage after light irradiation and increase in both protochlorophyllide and PPIX contents suggesting herbicidal effect of ALA (Matsumoto et al, 1994 ). In the other hand, ALA-treated plants significantly upregulated transcript levels of genes encoding superoxide dismutase and serine/threonine kinase receptors but the induction of antioxidative components lacked capacity to withstand ROS generation (Phung and Jung, 2014 , 2015 ). In 2004, Jung and co-workers shed light on “photodynamic stress” as they showed that rice plants suffered from severe oxidative damage upon the ectopic expression of the bacterial ALA synthase gene bringing about the accumulation of harmful photosensitizers PPIX and protochlorophyllide under 350 μmol.m −2 .s −1 (Jung et al, 2004 ).…”

Section: Indirect Photodynamic Stressmentioning

confidence: 99%

“…They constitute the main class of PPO-inhibiting herbicides that are widely investigated. Phung and Jung ( 2015 ) reported the different responses of photodynamically stressed rice plants undergoing ALA (5 mM) and oxyfluorfen (50 μM) herbicidal treatment. In term of phenotype under illumination, ALA induced bleached necrotic spots while oxyfluorfen caused bronzed necrotic spots on the leaves.…”

Section: Indirect Photodynamic Stressmentioning

confidence: 99%

Plant Photodynamic Stress: What's New?

2018

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In the 1970's, an unconventional stressful photodynamic treatment applied to plants was investigated in two directions. Exogenous photosensitizer treatment underlies direct photodynamic stress while treatment mediating endogenous photosensitizer over-accumulation pinpoints indirect photodynamic stress. For indirect photodynamic treatment, tetrapyrrole biosynthesis pathway was deregulated by 5-aminolevulenic acid or diphenyl ether. Overall, photodynamic stress involves the generation of high amount of reactive oxygen species leading to plant cell death. All these investigations were mainly performed to gain insight into new herbicide development but they were rapidly given up or limited due to the harmfulness of diphenyl ether and the high cost of 5-aminolevulinic acid treatment. Twenty years ago, plant photodynamic stress came back by way of crop transgenesis where for example protoporphyrin oxidases from human or bacteria were overexpressed. Such plants grew without dramatic effects of photodamage suggesting that plants tolerated induced photodynamic stress. In this review, we shed light on the occurrence of plant photodynamic stress and discuss challenging issues in the context of agriculture focusing on direct photodynamic modality. Indeed, we highlighted applications of exogenous PS especially porphyrins on plants, to further develop an emerged antimicrobial photodynamic treatment that could be a new strategy to kill plant pathogens without disturbing plant growth.

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Differential antioxidant defense and detoxification mechanisms in photodynamically stressed rice plants treated with the deregulators of porphyrin biosynthesis, 5-aminolevulinic acid and oxyfluorfen

Cited by 15 publications

References 33 publications

Differential Antioxidant Responses and Perturbed Porphyrin Biosynthesis after Exposure to Oxyfluorfen and Methyl Viologen in Oryza sativa

Differential Antioxidant Responses and Perturbed Porphyrin Biosynthesis after Exposure to Oxyfluorfen and Methyl Viologen in Oryza sativa

Substrate Application of 5-Aminolevulinic Acid Enhanced Low-temperature and Weak-light Stress Tolerance in Cucumber (Cucumis sativus L.)

Plant Photodynamic Stress: What's New?

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