Differential Antibody Responses in 6.B Major Histocompatibility (B) Complex Congenic Chickens

Abstract

Lines 6.6-2 (B2B2) and 6.15-5 (B2B2), congenic for the major histocompatibility (B) complex with > 99.9% background gene uniformity, were used to examine primary antibody responses to two antigens. In each of two trials, 1 mL of 5% SRBC, a T cell-dependent antigen, or 0.1 mL of Brucella abortus (BA), a T cell-independent antigen, was injected into separate groups of each B genotype aged 3 and 6 wk. Blood samples were taken from the chickens 7 d after immunization. Serum titers (log2) for both total antibody and mercaptoethanol (ME)-sensitive antibody to detect IgG were assayed by microtiter procedures. Least squares analysis of variance and Fisher's protected Least Significant Difference at P < 0.05 were used to evaluate the data. The total anti-SRBC antibody titer was significantly higher in B5B5 chicks than in B2B2 chicks at 4 and 7 wk of age. There was no significant difference in ME sensitive antibody to SRBC. Chicks of the B5B5 genotype also had significantly higher total and IgG antibody titers to BA at both ages than B2B2 chicks. The results indicate that 4- and 7-wk-old B5B5 chicks had a significantly stronger antibody response to SRBC or BA than B2B2 chicks.

Bee pollen and propolis as dietary supplements for rabbit: Effect on reproductive performance of does and on immunological response of does and their offspring

“…At 10 days post‐immunization on dams and at 90 days of age on all the born kits, ~2.0 ml blood samples were collected. The levels of anti‐SRBCs antibody titres were determined using a microhaemagglutination technique as described by Yamamoto and Glick () and Dix and Taylor (). The antibody titre values were expressed as log 2 of the highest serum dilution giving total agglutination to sheep red blood cells.…”

“…The rabbits were housed in a naturally ventilated building and kept in individual Italian wire galvanized cages (60 × 55 × 40 cm) equipped with an internal nest box. The rabbits were fed ad libitum with a commercial pelleted diet (Table 1) Yamamoto and Glick (1982) and Dix and Taylor (1996). The antibody titre values were expressed as log 2 of the highest serum dilution giving total agglutination to sheep red blood cells.…”

Estimation of digestible tryptophan:lysine ratios for maximum performance, egg quality and welfare of white-egg-laying hens by fitting the different non-linear models

“…Blood samples were collected from each bird at 12 d after SRBC challenge. The total, mercaptoethanol-sensitive (MES, presumably IgM) and mercaptoethanol-resistant (MER, presumably IgG) anti-SRBC antibody titres were determined using a microheamagglutination technique as described by Yamamoto and Glick (1982) and Dix and Taylor (1996). The antibody data were expressed as the log 2 of the reciprocal of the highest dilution giving visible agglutination.…”

Effects of various levels of organic acids and of virginiamycin on performance, blood parameters, immunoglobulins and microbial population of broiler chicks

“…The colon was removed and its contents were streaked on agar plates for culture. The total immunoglobulin M (IgM) and Y (IgY) titers were determined as described by Dix & Taylor (1996). To determine bacterial growth and colony counts, the agar collecting tubes were weighed, wrapped in an aluminium sheet, and autoclaved for 10 min.…”