Differential and tissue-specific activation pattern of the AtPROPEP and AtPEPR genes in response to biotic and abiotic stress in Arabidopsis thaliana

Abstract

In Arabidopsis thaliana At PEPR1 and At PEPR2 act as the receptors for the endogenous At PROPEP-derived Pep peptides and subsequently initiate defense-signaling cascades. In the previous work, 9 the expression pattern of the genes encoding the PEPR receptors and the AtPROPEP peptide precursor proteins was studied using promoter-GUS reporter constructs. Here, using the same constructs to study their expression pattern under biotic and abiotic stress, including At Pep1, flg22, methyl jasmonate (MeJA), and NaCl treatments, we observed that in response to At Pep1 and flg22, the activation of AtPEPR1 promoter was different from AtPEPR2 . We also found that these promoters were differentially activated in response to NaCl. Remarkably, we showed that it is possible to classify the genes of the AtPROPEP family, based on the response of their promoters to the various stimuli employed: thus, we classify AtPROPEP1 in one group; AtPROPEP2 and AtPROPEP3 in a second group; AtPROPEP4, AtPROPEP7 and AtPROPEP8 in a third group and AtPROPEP5 in a fourth group. Our finding, confirm non-redundant roles among the members of the AtPROPEP family and their corresponding receptors.

Retracted Article: Investigation of yellow horn (Xanthoceras sorbifoliaBunge) transcriptome in response to different abiotic stresses: a comparative RNA-Seq study

“…Furthermore, recent advances in biotechnology have also accelerated the molecular understanding of plants in response to different abiotic stresses. 38 As a result, a huge number of stress-responsive genes have been screened and functionally annotated in model plants such as Arabidopsis, 47 Oryza sativa 48 and Zea mays. 49 Nevertheless, little information can be available on the molecular responses of yellow horn with regard to salt stress, ABA stress and low temperature stress.…”

Maintenance of Cell Wall Integrity under High Salinity

“…The CWD caused by salinity stress, isoxaben, an inhibitor of cellulose biosynthesis, or driselase, a cell wall-degrading enzyme, can increase the protein levels of hormone-like peptides PROPEP1/3, the precursors of plant elicitor peptide 1/3 (Pep1/3) [ 138 , 147 ]. The Pep3 knockdown plants and the null mutant of Pep1 receptor 1 ( PEPR1 ) both exhibit salt-hypersensitivity [ 148 ].…”

Prunus persica plant endogenous peptides PpPep1 and PpPep2 cause PTI-like transcriptome reprogramming in peach and enhance resistance to Xanthomonas arboricola pv. pruni

“…An extensive transcriptional reprogramming is activated in a matter of hours [ 22 ], and cell wall re-modelling and synthesis of antimicrobial products is triggered within days [ 23 , 24 ]. Interestingly, Peps seem to have positive feedback since AtPep1 induces expression of its own precursor, so amplifying the pattern-triggered immunity (PTI) response [ 1 , 25 ]. This response has been specially studied in AtPeps, as they not only play a critical role in immunity, but may also be involved in development, and other biological processes from germination to flowering and seed production [ 25 – 27 ].…”