Differential activities of maize plant elicitor peptides as mediators of immune signaling and herbivore resistance

Abstract

Plant elicitor peptides (Peps) are conserved regulators of defense responses and models for the study of damage-associated molecular pattern-induced immunity. Although present as multigene families in most species, the functional relevance of these multigene families remains largely undefined. While Arabidopsis Peps appear largely redundant in function, previous work examining Pep-induced responses in maize (Zm) implied specificity of function. To better define the function of individual ZmPeps and their cognate receptors (ZmPEPRs), activities were examined by assessing changes in defense-associated phytohormones, specialized metabolites and global gene expression patterns, in combination with heterologous expression assays and analyses of CRISPR/Cas9-generated knockout plants. Beyond simply delineating individual ZmPep and ZmPEPR activities, these experiments led to a number of new insights into Pep signaling mechanisms. ZmPROPEP and other poaceous precursors were found to contain multiple active Peps, a phenomenon not previously observed for this family. In all, seven new ZmPeps were identified and the peptides were found to have specific activities defined by the relative magnitude of their response output rather than by uniqueness. A striking correlation was observed between individual ZmPep-elicited changes in levels of jasmonic acid and ethylene and the magnitude of induced defense responses, indicating that ZmPeps may collectively regulate immune output through rheostat-like tuning of phytohormone levels. Peptide structure-function studies and ligand-receptor modeling revealed structural features critical to the function of ZmPeps and led to the identification of ZmPep5a as a potential antagonist peptide able to competitively inhibit the activity of other ZmPeps, a regulatory mechanism not previously observed for this family.

A sugarcane mosaic virus vector for rapid in planta screening of proteins that inhibit the growth of insect herbivores

“…Plant elicitor peptides (Peps) trigger anti‐herbivore defence responses (Huffaker, 2015 ; Huffaker et al ., 2013 ; Poretsky et al ., 2020 ). In maize, Zm Pep1 and Zm Pep3 up‐regulate defences, at least in part by induction of the jasmonic acid (JA) signalling pathway (Huffaker et al ., 2011 , 2013 ).…”

The race goes on: A fall armyworm-resistant maize inbred line influences insect oral secretion elicitation activity and nullifies herbivore suppression of plant defense

“…On the other hand, ZmPROPEP3 (Zm00001d002138) expression was almost 100-fold higher in Xi502 than B73 under both constitutive and induced conditions (Table S1). Since this peptide has been recently demonstrated to have the strongest elicitation effect, the relatively low absolute expression level (compared to ZmPROPEP2) may be sufficient to induce significant defense responses nevertheless (Poretsky et al ., 2020).…”

Phytocytokines function as immunological modulators of plant immunity

“…PEP1, a 23-aa peptide, is the first phytocytokine identified in Arabidopsis. Arabidopsis genome encodes eight PEPs, and their orthologs have been identified in a wide array of plant species, including maize, rice, potato, and soybean (Huffaker et al 2011;Huffaker et al 2006;Poretsky et al 2020). AtPEP1 activates the hallmark of PTI responses, promotes plant resistance to various pathogens, including bacterium Pseudomonas syringae, fungus Botrytis cinerea and oomycete Phytophthora infestans (Huffaker et al 2006;Yamaguchi et al 2010;Liu et al 2013).…”