Differential accumulation of vimentin fragments in preeclamptic placenta

Mary, Sheon; Kulkarni, Mahesh J.; Mehendale, Savita; Joshi, Sadhana; Giri, Ashok P.

doi:10.1002/cm.21390

Cited by 3 publications

(4 citation statements)

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“…The clarification and prediction of the placental pathophysiology and biochemical process and bio-marker determination in preeclamptic patients is necessary for the clinical care in these patients. Vimentin can be studied as a predictive marker in the prediction of preeclampsia (5). In our study, vimentin was found to be significantly higher in the serum levels of the preeclamptic patients when compared to the normal pregnant women.…”

Section: Figure 1: Comparison Of Vimentin Level Between Preeclamptic and Normotensive Pregnant Womenmentioning

confidence: 47%

“…gastrulation and embryogenesis, is a process in which the phenotype of the epithelial cells changes and turns into mesenchymal cells (8). The pathology in the mesenchymal transformation causes poor invasion and poor placentation (5). Vimentin, on the other hand, is an important mesenchymal marker indicating the disappearance of the cell adhesion.…”

Section: Introductionmentioning

confidence: 99%

“…However, the placenta constitutes the main source in the pathophysiology of the disease (4). Preeclampsia is assumed to occur with the mechanism resulting from the remodeling of the spiral arteries caused by angiogenic intermolecular imbalance and trophoblastic invasion disorder (5). As a result, blood flow to the placenta is disrupted and the hypoxic placenta is exposed to oxidative stress with decreasing blood flow.…”

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confidence: 99%

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Preeklamptik ve Normotansif Gebeler Arasındaki Vimentin Seviyelerinin Karşılaştırılması

Yılmaz

Topdaği

Aşkın

2020

Jinekoloji-Obstetrik Ve Neonatoloji Tıp Dergisi

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Background: Hypertensive diseases in pregnancy are one of the leading causes of maternal and perinatal mortality worldwide. The aim of our study to compare the serum vimentin levels between the pregnant women diagnosed with preeclampsia (PE) and the normal pregnant women. Materials and Methods: This sectional study was conducted on 40 pregnant women diagnosed with preeclampsia and 21 healthy pregnant women. The maternal serum vimentin levels were evaluated via the sandwich enzyme immunoassay method. Results: The mean vimentin levels were found significantly different between the groups. (47.25±29.74 vs.19.39±3.76 p=0.000) No significant difference was discovered between the two groups in respect of the gestational week, platelet level, parity and body mass index (BMI) during the diagnosis (p>0.05). Conclusion: Findings demonstrate that vimentin plays a role in the pathogenesis of preeclampsia. However, further studies, in which the number of patients is high and subgroups are compared, are needed.

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Section: Figure 1: Comparison Of Vimentin Level Between Preeclamptic and Normotensive Pregnant Womenmentioning

confidence: 47%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Preeklamptik ve Normotansif Gebeler Arasındaki Vimentin Seviyelerinin Karşılaştırılması

Yılmaz

Topdaği

Aşkın

2020

Jinekoloji-Obstetrik Ve Neonatoloji Tıp Dergisi

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“…Also, this mechanism has been reported in most of cancers [ 24 – 26 ]. The expression level of Vimentin is positively correlated with cell invasion, especially in epithelial cancers and preeclampsia placenta [ 27 , 28 ]. Whereas, Vimentin is the essential protein of cellular intermediate filaments, its expression change will affect the cell shape, the ability of cell migration, cell adhesion and cell signaling activities accordingly.…”

Section: Discussionmentioning

confidence: 99%

High TXNIP expression accelerates the migration and invasion of the GDM placenta trophoblast

Yang

et al. 2023

BMC Pregnancy Childbirth

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Introduction Our previous study has proofed the glucose sensitive gene-thioredoxin-interacting protein (TXNIP) expression was up in the placenta of the patients with gestational diabetes mellitus (GDM), but the pathological mechanisms underlying abnormal TXNIP expression in the placenta of patients with GDM is completely unclear and additional investigations are required to explain the findings we have observed. In the present study, we simulated the high TXNIP expression via introducing the Tet-On “switch” in vitro, approximate to its expression level in the real world, to explore the following consequence of the abnormal TXNIP. Methods The expression and localization of TXNIP in the placenta of GDM patients and the health control was investigated via immunofluorescent staining, western blot and RT-qPCR. Overexpression of TXNIP was achieved through transfecting Tet-on system to the human trophoblastic cell line-HTR-8/Svneo cell. TXNIP knockout was obtained via CRISPR-Cas9 method. The cell phenotype was observed via IncuCyte Imaging System and flow cytometry. The mechanism was explored via western blot and RT-qPCR. Results The expression level of TXNIP in the GDM placenta was nearly 2–3 times higher than that in the control. The TXNIP located at trophoblastic cells of the placenta. When the expression of TXNIP was upregulated, the migration and invasion of the cells accelerated, but cell apoptosis and proliferation did not changed compared with the control group. Furthermore, the size of the TetTXNIP cells became larger, and the expression level of Vimentin and p-STAT3 increased in the TetTXNIP cells. All the changes mentioned above were opposite in the TXNIP-KO cells. Conclusions Abnormal expression of TXNIP might be related to the impairment of the GDM placental function, affecting the migration and invasion of the placental trophoblast cells through STAT3 and Vimentin related pathway; thus, TXNIP might be the potential therapeutic target for repairing the placental dysfunction deficient in GDM patients.

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