Different Transcriptional Profiles of Human Monocyte-Derived Dendritic Cells Infected with Distinct Strains ofMycobacterium tuberculosisandMycobacterium bovisBacillus Calmette-Guérin

Abstract

In order to analyze dendritic cells (DCs) activation following infection with different mycobacterial strains, we studied the expression profiles of 165 genes of human monocyte-derived DCs infected with H37Rv, a virulent Mycobacterium tuberculosis (MTB) laboratory strain, CMT97, a clinical MTB isolate, Mycobacterium bovis bacillus Calmette-Guérin (BCG), Aventis Pasteur, and BCG Japan, both employed as vaccine against tuberculosis. The analysis of the gene expression reveals that, despite a set of genes similarly modulated, DCs response resulted strain dependent. In particular, H37Rv significantly upregulated EBI3 expression compared with BCG Japan, while it was the only strain that failed to release a significant IL-10 amount. Of note, BCG Japan showed a marked increase in CCR7 and TNF-α expression regarding both MTB strains and it resulted the only strain failing in exponential intracellular growth. Our results suggest that DCs display the ability to elicit a tailored strain-specific immune response.

Mycobacterium tuberculosis infection of human dendritic cells decreases integrin expression, adhesion and migration to chemokines

“…This indicates that they possess the ability to respond to the above chemokines and migrate accordingly; further highlighting that this inhibition is exclusive to M. tuberculosis and is suggestive of a mechanism independent of CCR7. This is in contrast to reports of mycobacteria inhibiting chemotaxis of DCs through a block in the up‐regulation of CCR7 . Our data also suggest a mechanism by which a loss of surface‐expressed integrins impacts migration.…”

Different Transcriptional Profiles of RAW264.7 Infected with Mycobacterium tuberculosis H37Rv and BCG Identified via Deep Sequencing

“…However, the opportune response of host to Mycobacterium tuberculosis and the antigens they confront are still unclear. Simultaneously, the innate immune response to pathogenic bacteria and the subsequent adaptive immune response to eliminate the pathogens which can protect the host from pathogenic bacterial invasion have not been expound systematically [7].…”

Effects of Leishmania major clones showing different levels of virulence on infectivity, differentiation and maturation of human dendritic cells

“…However, to our knowledge, this is the first demonstration of a significant difference in the in‐vitro infectivity of human DCs by Lm strains differing by their virulence. Recently, it was reported that DCs control the intracellular growth of mycobacteria strains differently, suggesting variability in the cell‐to‐cell spread outcome during the first step of infection [26].…”

“…An asterisk indicates a statistically significant difference between uninfected and LPS-matured DCs (P < 0·05); **statistically significant difference between uninfected and IFN-g/LPS-matured DCs (P < 0·05). growth of mycobacteria strains differently, suggesting variability in the cell-to-cell spread outcome during the first step of infection [26].…”