Different Stimulating Capacity of Monocytes and B Lymphocytes in Mixed Leukocyte Cultures: A Dose Response Study

Abstract

Cell fractions highly enriched either for monocytes or for B lymphocytes were separated from human peripheral blood. They were tested for their capacity to stimulate allogeneic lymphocytes in mixed leukocyte cultures, using dose‐response titrations. Neither population could be stimulated to proliferate by allogeneic cells, but both proved strong stimulators. In this respect monocytes were more efficient than B cell preparations. Differences were pronounced at the lower numbers of stimulating cells but were no longer significant with large amounts of cells.