Special Susceptibility to Apoptosis of CD1a<sup>+</sup>Dendritic Cell Precursors Differentiating from Cord Blood CD34<sup>+</sup>Progenitors

Canque, Bruno; Camus, Sandrine; Yagello, Micaël; Gluckman, Jean Claude

doi:10.1002/stem.160218

Cited by 20 publications

(11 citation statements)

References 37 publications

(66 reference statements)

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“…5C), which resulted in a markedly decreased cell survival after 96 h compared with control cultures (77.4% decreased CD14 ϩ cell survival compared with control). Although CD1a ϩ -sorted cells showed a higher spontaneous apoptosis, as reported previously (18), these cells were almost insensitive to Dex-induced apoptosis (Fig. 5C).…”

Section: Dex Prevents Final Differentiation Of Cd14 ϩ DC Precursors Tsupporting

confidence: 87%

Corticosteroids Prevent Generation of CD34+-Derived Dermal Dendritic Cells But Do Not Inhibit Langerhans Cell Development

Woltman

Massacrier²,

Fijter

et al. 2002

The Journal of Immunology

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Corticosteroids (CS) have been shown to exert strong inhibitory effects on dendritic cell (DC) differentiation and function. Those studies were mostly performed with monocyte-derived DC, which represents only one subpopulation from the wide variety of DC types. In the present study the effects of the CS dexamethasone and prednisolone were investigated on the differentiation of CD34+ hemopoietic progenitor cells into 1) Langerhans cells (LC), which differentiate directly into CD1a+ DC; and 2) dermal/interstitial DC, which differentiate via a CD14+CD1a− phenotype into CD14−CD1a+ DC. CS present during the entire 11-day culture period, resulting in fully differentiated CD1a+ DC, increased the percentage of langerin+ DC within the CD1a+ population. In line with these data, CS treatment during the first 6 days of differentiation reduced the development of CD14+ dermal DC precursors and thereby seemed to support the generation of CD1a+ LC precursors. Addition of CS from day 6 onward specifically blocked the development of CD1a+ dermal DC by both inhibition of spontaneous and IL-4-induced differentiation of CD14+ DC precursors into CD1a+ DC as well as induction of apoptosis in CD14+ DC precursors. Apoptosis was not found in CD14+ macrophage precursors derived from the same CD34+ progenitors. The development and function of LC were not affected by CS, as demonstrated by a normal T cell stimulatory capacity and IL-12 production. These data demonstrate that CS interfere with the normal development of DC from CD34+ progenitors by specific induction of apoptosis in precursors of dermal/interstitial DC. In view of the different functional capacities of dermal/interstitial DC and Langerhans cells, this might affect the overall cellular immune response.

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Section: Dex Prevents Final Differentiation Of Cd14 ϩ DC Precursors Tsupporting

confidence: 87%

Corticosteroids Prevent Generation of CD34+-Derived Dermal Dendritic Cells But Do Not Inhibit Langerhans Cell Development

Woltman

Massacrier²,

Fijter

et al. 2002

The Journal of Immunology

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“…The arrows indicate transcripts unique to placenta sample 4. b, inter-individual variation in the expression of DC-SIGN2 transcripts in placenta. DC-SIGN2-specific primers were used to PCR amplify oligo(dT)-primed cDNAs from 10 different individuals (donors [5][6][7][8][9][10][11][12][13][14], and the products were analyzed as described above. Note the actin controls for the cDNA synthesized from each donor.…”

Section: Discussionmentioning

confidence: 99%

“…How this happens is not precisely known. However, there is growing support for the notion that dendritic cells (DCs) present within the mucosal sites may play a central role in this process (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15). The normal function of DCs is to survey mucosal surfaces for antigens, capture the antigens, process captured proteins into immunogenic peptides, emigrate from tissues to the paracortex of draining lymph nodes, and present peptides in the context of MHC (major histocompatibility complex) molecules to T cells (1).…”

mentioning

confidence: 99%

Extensive Repertoire of Membrane-bound and Soluble Dendritic Cell-specific ICAM-3-grabbing Nonintegrin 1 (DC-SIGN1) and DC-SIGN2 Isoforms

Mummidi¹,

Catano²,

Lam³

et al. 2001

Journal of Biological Chemistry

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“…Although DCs express CD4 and CCR5 at low levels, productive infection of DCs occurs infrequently (3,5,(8)(9)(10)(11)(12). Furthermore, DCs do not express CXCR4 and have proven refractory to infection with CXCR4-tropic isolates of HIV-1 (13,14). Alternatively, capture of virus particles by HIV-attachment factors, such as C-type lectin receptors expressed on varied DC subsets (15), results in endocytosis of virus particles.…”

mentioning

confidence: 99%

Immature dendritic cell-derived exosomes can mediate HIV-1 trans infection

Wiley

Gummuluru

2006

Proc. Natl. Acad. Sci. U.S.A.

283

258

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Immature dendritic cells (DCs) capture HIV type 1 (HIV-1) and can transmit captured virus particles to T cells. In this report, we show that HIV-1 particles captured by DCs can be transmitted to T cells by exocytosis without de novo infection. Captured HIV-1 particles were rapidly endocytosed to tetraspan protein (CD9, CD63)-positive endocytic compartments that were reminiscent of multivesicular endosomal bodies. Furthermore, some of the endocytosed virus particles were constitutively released into the extracellular milieu in association with HLA-DR1 ؉ , CD1b ؉ , CD9 ؉ , and CD63 ؉ vesicles (exosomes) and could initiate productive infections of CD4 ؉ target cells. Surprisingly, the exocytosed vesicle-associated HIV-1 particles from DCs were 10-fold more infectious on a perparticle basis than cell-free virus particles. These studies describe a previously undescribed mechanism of DC-mediated HIV-1 transmission and suggest that virus particle trafficking to multivesicular endosomal bodies and subsequent exocytosis can provide HIV-1 particles captured by DCs an avenue for immune escape.

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Special Susceptibility to Apoptosis of CD1a⁺Dendritic Cell Precursors Differentiating from Cord Blood CD34⁺Progenitors

Abstract: We analyzed the effect of tumor necrosis factor (TNF)-α on the differentiation and viability of dendritic cells (DC) generated from cord blood CD34

Cited by 20 publications

References 37 publications

Corticosteroids Prevent Generation of CD34+-Derived Dermal Dendritic Cells But Do Not Inhibit Langerhans Cell Development

Corticosteroids Prevent Generation of CD34+-Derived Dermal Dendritic Cells But Do Not Inhibit Langerhans Cell Development

Extensive Repertoire of Membrane-bound and Soluble Dendritic Cell-specific ICAM-3-grabbing Nonintegrin 1 (DC-SIGN1) and DC-SIGN2 Isoforms

Immature dendritic cell-derived exosomes can mediate HIV-1 trans infection

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Special Susceptibility to Apoptosis of CD1a+Dendritic Cell Precursors Differentiating from Cord Blood CD34+Progenitors

Abstract: We analyzed the effect of tumor necrosis factor (TNF)-α on the differentiation and viability of dendritic cells (DC) generated from cord blood CD34

Cited by 20 publications

References 37 publications

Corticosteroids Prevent Generation of CD34+-Derived Dermal Dendritic Cells But Do Not Inhibit Langerhans Cell Development

Corticosteroids Prevent Generation of CD34+-Derived Dermal Dendritic Cells But Do Not Inhibit Langerhans Cell Development

Extensive Repertoire of Membrane-bound and Soluble Dendritic Cell-specific ICAM-3-grabbing Nonintegrin 1 (DC-SIGN1) and DC-SIGN2 Isoforms

Immature dendritic cell-derived exosomes can mediate HIV-1 trans infection

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Product

Resources

About

Special Susceptibility to Apoptosis of CD1a⁺Dendritic Cell Precursors Differentiating from Cord Blood CD34⁺Progenitors