Different stages of B cell differentiation in non-T acute lymphoblastic leukemia.

Abstract

Abstract. Immunoglobulin heavy chain gene rearrangement was evaluated in 19 cases of acute lymphoblastic leukemia (ALL) and correlated with the immunological phenotypic expression on primary or phorbol diester (12-0-tetradecanoylphorbol-1 3-acetate[TPA])-induced cells. One case of common ALL (cALL), one case of T-ALL, and one undifferentiated acute leukemia that responded to anti-myeloid drugs after unsuccessful anti-lymphoid induction therapy, had germ line heavy chain genes. Rearranged immunoglobulin genes were instead found in 15 of the 16 cALL cases studied and in a case of non-T, non-B, non-common ("null") ALL, which suggested the B cell origin of the neoplastic cells.All cases bearing a heavy chain gene rearrangement were HLA-DR positive. However, the unique cALL case with a germ line configuration was also HLA-DR positive, which confirmed that both the cALL antigen and HLA-DR antigen were not per se expression of B cell commitment. On the other hand, a complete search for B cell-related markers (BA-1 and B1 monoclonal antibodies, as well as cytoplasmic immunoglobulins [CyIg]) in the cALL cases showed that at least one B cell marker could be detected either on primary or on TPA-induced cells in all cases in which a gene rearrangement had occurred. Incubation with TPA allowed the detection of one B cell marker in a case in which the primary cells were negative, and increased the expression

Lineage-related polypeptide markers in acute lymphoblastic leukemia detected by two-dimensional gel electrophoresis.

“…In contrast, the pattern for four of six B-cell lineage (LB) markers was the same in cALL as for B cells and/or B-cell ALL. These observations are consistent with and reinforce findings based on immunoglobulin gene rearrangement and cell-surface markers that suggest a B-cell origin of cALL (8,14,25).…”

Nonrandom TRGγ variable gene rearrangement in normal human T cells and T cell leukemias

“…Usually, 95% pure blasts were obtained. Restriction enzyme analysis, Southern blot electrophoresis and hybridization to a 32P-nick-translated probe were performed as previously described [31]. The probe used to detect TRGy rearrangements is a 0.7-kb Hind 111-Eco RI genomic fragment containing the J,1 region [27], which is located a short distance upstream of the C,1 gene [9].…”

A human lymphoma cell line with multiple immunoglobulin rearrangements.

“…The original stud- Receivedfor publication 4 March 1991 and in revisedform 22 October 1991. ies have been confirmed and extended by a large number of groups (2)(3)(4)(5). In most cases only one or two rearranged bands are seen (1); however, in some cases three or more rearranged bands can be identified (6)(7)(8)(9). Various explanations have been proposed; these range from the suggestion that all of the rearranged bands are present in all ofthe cells to the suggestion that the disease is in fact oligoclonal and that individual clones carry unique rearrangements.…”