A human lymphoma cell line with multiple immunoglobulin rearrangements.

Abstract

The development of a cell culture system efficient in the establishment of lymphoma cell lines has made it possible to dissect basic biological and molecular aspects of lymphoma cells. We have established a lymphoma cell line from a patient with B cell lymphoma. The cell line has a complex karyotype with translocations involving bands 8q24, 14q32, and 18q21. Molecular analysis revealed that the Myc gene was rearranged; we were unable to demonstrate rearrangement ofthe Bcl-2 gene. Evaluation of the structure of the heavy chain Ig genes revealed that the cell line carried the same rearrangements as the cells from which the cell line was derived. The pattern of rearrangement, however, was unusual in that there were at least four rearranged bands when DNA cut with HindIII was probed with a fragment of the heavy chain joining region. To further characterize the cell line, subclones were derived. Individual subclones had the same pattern of rearrangement as the parent cell line. The results of these studies provide evidence that multiple rearranged Ig genes may be present in a single clone of cells. (J.

Molecular cytogenetic characterization of non‐Hodgkin lymphoma cell lines

“…The most frequently gained chromosomes were 1, 7, 11, and 18. The karyotype of OCI‐Ly18 has been published previously with a modal chromosome number of 52 (Chang et al, 1992). This cell line has been propagated for many years, and the model chromosome number of the line carried in our laboratory was 96–98 with 11 copies of chromosome 18; two of each were involved in t(8;18;14)(q24;q21;q32) and t(8;18)(q24;q21) translocations.…”

MASL1, a candidate oncogene found in amplification at 8p23.1, is translocated in immunoblastic B-cell lymphoma cell line OCI-LY8

“…As previously reported, translocations of the BCL2, BCL6, and MYC genes with IgH gene are present in OCI-LY8 (Chang et al, 1995;Chaganti et al, 1998), and it is well established that these translocations play pivotal roles in lymphomagenesis. However, it is conceivable that gene alterations of MASL1 as a result of chromosome translocation may play an additional role in lymphomagenesis because MASL1 was shown to have tumorigenic activity.…”

B‐cell lymphomagenesis in SIV‐immunosuppressed cynomolgus monkeys

“…The VDJ rearrangement pattern of one cell line differed from that of the corresponding lymphoma, indicating loss of one clone from the oligoclonal tissue during establishment of the cell line. Thus this oligoclonal lymphoma did not represent a single clone with several immunoglobulin rearrangements (Chang et al, 1992). Our results show that PCR for VDJ rearrangements of the human IgH gene is also sensitive in studies of simian B-cell lymphomas allowing detection of B-cell clones corresponding to approx.…”