Different roles of crayfish hemocytes in the uptake of foreign particles

Li, Fang; Chang, Xuefeng; Xu, Limei; Yang, Feng

doi:10.1016/j.fsi.2018.03.029

Cited by 39 publications

(37 citation statements)

References 27 publications

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“…Base on this method, hemocytes in some shellfishes were classified into two 43,44 , three 22,23 and four 45 types. FCS and SSC are sometimes not reflecting actual FCS/SCC in crustaceans by FCM 46 , thus the matches between microscopic images and FCM may not be reached exactly. In the present study, hemocytes of the Chinese horseshoe crab and mangrove horseshoe crab, for males and females, were classified into three groups (G1 cells, G2 cells and G3 cells, probably are hyaline cells, semi-granular cells and granular cells).…”

Section: Discussionmentioning

confidence: 99%

Classification and characterization of hemocytes from two Asian horseshoe crab species Tachypleus tridentatus and Carcinoscorpius rotundicauda

Xie

Yan

et al. 2019

Sci Rep

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In present study, transmission electron microscopy and flow cytometry were utilized to investigate the classification, characterization and immune functions of hemocytes from horseshoe crab, Tachypleus tridentatus and Carcinoscorpius rotundicauda . Three types of hemocytes were distinguished respectively: the granular cell, the semi-granular cell and the hyaline cell by transmission electron microscopy, while three hemocyte subpopulations (Gate 1 cell, Gate 2 cell, Gate 3 cell) were classified by flow cytometry. Hyaline cell was the major cell type with the highest nuclear-cytoplasmic ratio and granular cell and semi-granular cell showed lower ratios. Immune parameters of hemocytes in horseshoe crabs were investigated by flow cytometry. Different hemocyte subpopulations respond for diverse functions. Lysosomal contents and hemocyte mortality in Gate 3 cell subpopulation were higher than that in other subpopulations, while reactive oxygen species, phagocytosis and non-specific esterase, in Gate 1 cell subpopulation, were higher than those in other subpopulations. The hemocyte types between the two species had no significant differences in staining or morphology.

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Section: Discussionmentioning

confidence: 99%

Classification and characterization of hemocytes from two Asian horseshoe crab species Tachypleus tridentatus and Carcinoscorpius rotundicauda

Xie

Yan

et al. 2019

Sci Rep

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“…They have also proven to be the main sites for the storage and release of antimicrobial peptides and lectins. The hyalinocytes are considered the main phagocytes ( 15 , 75 ). It was reported that hemocyte subpopulations also had differential responses to pathogen invasion.…”

Section: Discussionmentioning

confidence: 99%

Differential Apoptotic Responses of Hemocyte Subpopulations to White Spot Syndrome Virus Infection in Fenneropenaeus chinensis

et al. 2020

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The apoptosis of hemocytes plays an essential function in shrimp immune defense against pathogen invasions. In order to further elucidate the differential apoptotic responses of the granulocytes and the hyalinocytes in Fenneropenaeus chinensis post WSSV infection, the characteristics of apoptotic dynamics and viral proliferation in total hemocytes and hemocyte subpopulations were respectively investigated in the present work. The results showed that the apoptotic rate of hemocytes changed significantly, and the apoptosis-related genes also showed significantly differential expression responses during WSSV infection. Interestingly, we found that the apoptotic rate of virus-negative hemocytes was significantly higher than that of virus-positive hemocytes in the early stage of WSSV infection, while it was significantly lower than that of virus-positive cells in the middle and late infection stages. The difference of apoptosis between virus-positive and virus-negative hemocytes seems to be an important way for the WSSV to destroy the host’s immune system and facilitate the virus spread at different infection stages. It was further found that the apoptosis rate of granulocytes was always significantly higher than that of hyalinocytes during WSSV infection, indicating that granulocytes have a stronger apoptotic response to WSSV infection. Moreover, a higher viral load was detected in granulocytes, and the density of granulocytes decreased more rapidly post WSSV infection, indicating that the granulocytes are more susceptible and vulnerable to WSSV infection compared with the hyalinocytes. These results collectively demonstrated that the apoptotic response in shrimp hemocytes was significantly influenced by the WSSV infection, and the differential apoptotic response of granulocytes and hyalinocytes to WSSV indicated the differences of antiviral mechanisms between the two hemocyte subpopulations.

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“…Similarly, different subpopulation of hemocytes in diverse species or even in the same species of crustaceans also exhibited variable phagocytic activities. For instance, the higher phagocytic capacity has been observed in the hyaline cells rather than granular and semigranular cells of C. maenas, Carcinus aestuarii (15), and Eriocheir sinensis (16,17), while main phagocytic capabilities of granular and semigranular cells were observed in Macrobrachium rosenbergii, Penaeus monodon, and Cherax quadricarinatus (17)(18)(19)(20). Higher phagocytic activities were also demonstrated in the semigranular cells of signal crayfish P. leniusculus.…”

Section: Biological Characteristics Of Hemocytementioning

confidence: 96%

“…However, it is noteworthy to mention that different subpopulations of hemocytes seem to exhibit specific preferences in phagocytosis of different bacteria or viruses. For instance, Escherichia coli was mainly ingested and cleared by semigranular and granular cells, whereas WSSV was mostly ingested by semigranular cells in red claw crayfish C. quadricarinatus (20). Although phagocytosis has been found in different subpopulation of hemocytes in distinct species, thus far, it is still very difficult to clearly classify the high amounts of evolutionary diversity of crustaceans.…”

Section: Biological Characteristics Of Hemocytementioning

confidence: 99%

Hemocyte-Mediated Phagocytosis in Crustaceans

Liu

Zheng

et al. 2020

Front. Immunol.

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Phagocytosis is an ancient, highly conserved process in all multicellular organisms, through which the host can protect itself against invading microorganisms and environmental particles, as well as remove self-apoptotic cells/cell debris to maintain tissue homeostasis. In crustacean, phagocytosis by hemocyte has also been well-recognized as a crucial defense mechanism for the host against infectious agents such as bacteria and viruses. In this review, we summarized the current knowledge of hemocyte-mediated phagocytosis, in particular focusing on the related receptors for recognition and internalization of pathogens as well as the downstream signal pathways and intracellular regulators involved in the process of hemocyte phagocytosis. We attempted to gain a deeper understanding of the phagocytic mechanism of different hemocytes and their contribution to the host defense immunity in crustaceans.

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Different roles of crayfish hemocytes in the uptake of foreign particles

Cited by 39 publications

References 27 publications

Classification and characterization of hemocytes from two Asian horseshoe crab species Tachypleus tridentatus and Carcinoscorpius rotundicauda

Classification and characterization of hemocytes from two Asian horseshoe crab species Tachypleus tridentatus and Carcinoscorpius rotundicauda

Differential Apoptotic Responses of Hemocyte Subpopulations to White Spot Syndrome Virus Infection in Fenneropenaeus chinensis

Hemocyte-Mediated Phagocytosis in Crustaceans

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