Different Patterns of Physiological and Molecular Response to Drought in Seedlings of Malt‐ and Feed‐type Barleys (Hordeum vulgare)

Abstract

A number of physiological and molecular characteristics are proposed as selection criteria for drought tolerance. This study measured the associations between physiological and molecular characteristics of drought response in malting and fodder spring barleys. Plants of 13 malt‐ and 14 feed‐type Polish genotypes were exposed to drought at the four‐leaf stage for 7 days. Drought susceptibility indexes (DSI) were calculated for membrane integrity, water status, gas exchange and PSII photochemical activity. Accumulation of HVA1 and SRG6 transcripts in drought was measured with real‐time PCR. A wide range of variation in the drought response was observed among studied genotypes. Malting barleys were less sensitive to drought than feed‐barleys according to all the traits studied. In both groups, different patterns of relationships between traits were observed. In malting genotypes only, CO2 assimilation rates in drought, as well as PSII efficiency were related to both water content and the accumulation of HVA1 transcript in leaves. On the other hand the SRG6 expression was highly correlated in both groups of barley with the photochemical efficiency of PSII. The results suggest that different physiological, biochemical and molecular characteristics should be applied in the selection towards drought resistance in the case of malting and fodder barleys.

Application of photochemical parameters and several indices based on phenotypical traits to assess intraspecific variation of oat (Avena sativa L.) tolerance to drought

“…Chlorophyll a fluorescence kinetics (CF) allows determining whether the photosynthetic apparatus was damaged after cessation of watering. Fluorescence methods have been successfully applied in many studies related to plant drought resistance (Lichtenthaler and Babani 2004;Lichtenthaler et al 2005;Hura et al 2007;Rapacz et al 2010);Yin et al 2010). CF-modulated parameters are commonly used in the JIP-test.…”

Physiological adaptive mechanisms of plants grown in saline soil and implications for sustainable saline agriculture in coastal zone

“…1). O-J-I-P transient has been accepted as a reliable and popular tool in photosynthetic research, especially to study PSII behavior in recent years (Wen et al 2005;Strauss et al 2007;Toth et al 2007;Yang et al 2007;Chen et al 2008;Rapacz et al 2008;Yan et al 2008Yan et al , 2011Li et al 2009;Mehta et al 2010;Rapacz et al 2010;Jedmowski et al 2013). O, J, I and P are a sequence of phases in the kinetics of fast fluorescence rise, and environmental stress often provokes the occurrence of K phase in this transient, suggesting the damage to oxygen-evolving complex in donor side of PSII (Strasser et al 2004).…”

Drought-induced changes in the actin cytoskeleton of barley (Hordeum vulgare L.) leaves

“…Then the leaves were dried at 35°C (air drying chamber; Lumel, Zielona Góra, Poland) in darkness for 55, 110, 165, 220 or 275 min. Accumulation of ACT11, ADF1 and HVA1 transcripts was analyzed according to the protocol of Rapacz et al (2010) with substantial modifications. Barley sequences were found in GenBank: actin (ACT11)-GenBank ID: AY145451, late embryogenesis abundant protein (HVA1)-GenBank ID: FJ026804.1, ADP-ribosylation factor 1-like protein (ADP)-GenBank ID: AJ508228.2, actin depolymerization factor (ADF1)-GenBank ID: AK370859.1.…”

“…Barley (H. vulgare L.) could be used as an excellent experimental model addressing drought stresses encountered by crops in the field conditions (Rapacz et al 2010;Wójcik-Jagła et al 2012). As barley is an agronomically important species, its genome and physiological response to various stress conditions have been quite well characterized.…”