Drought-induced changes in the actin cytoskeleton of barley (Hordeum vulgare L.) leaves

Śniegowska-Świerk, Katarzyna; Dubas, Ewa; Rapacz, Marcin

doi:10.1007/s11738-015-1820-0

Cited by 25 publications

(13 citation statements)

References 35 publications

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“…It has been suggested that actin filaments (AFs) may participate in drought tolerance, as they form a network connecting cell walls, plasma membranes and cellular compartments. Through this physical connections they may act as osmotic sensors, because a decrease of turgor changes the compression of AFs, what in turn may be recognized by the cell as a signal to switch on expression of drought-responsive genes (Huang et al, 2012 ; Sniegowska-Swierk et al, 2015 , 2016 ). Previous studies of AFs in CamB and Maresi genotypes showed that these genotypes differ in actin content and AF organization, both in optimal water conditions and after leaf desiccation (Sniegowska-Swierk et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

No Time to Waste: Transcriptome Study Reveals that Drought Tolerance in Barley May Be Attributed to Stressed-Like Expression Patterns that Exist before the Occurrence of Stress

et al. 2018

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Plant survival in adverse environmental conditions requires a substantial change in the metabolism, which is reflected by the extensive transcriptome rebuilding upon the occurrence of the stress. Therefore, transcriptomic studies offer an insight into the mechanisms of plant stress responses. Here, we present the results of global gene expression profiling of roots and leaves of two barley genotypes with contrasting ability to cope with drought stress. Our analysis suggests that drought tolerance results from a certain level of transcription of stress-influenced genes that is present even before the onset of drought. Genes that predispose the plant to better drought survival play a role in the regulatory network of gene expression, including several transcription factors, translation regulators and structural components of ribosomes. An important group of genes is involved in signaling mechanisms, with significant contribution of hormone signaling pathways and an interplay between ABA, auxin, ethylene and brassinosteroid homeostasis. Signal transduction in a drought tolerant genotype may be more efficient through the expression of genes required for environmental sensing that are active already during normal water availability and are related to actin filaments and LIM domain proteins, which may function as osmotic biosensors. Better survival of drought may also be attributed to more effective processes of energy generation and more efficient chloroplasts biogenesis. Interestingly, our data suggest that several genes involved in a photosynthesis process are required for the establishment of effective drought response not only in leaves, but also in roots of barley. Thus, we propose a hypothesis that root plastids may turn into the anti-oxidative centers protecting root macromolecules from oxidative damage during drought stress. Specific genes and their potential role in building up a drought-tolerant barley phenotype is extensively discussed with special emphasis on processes that take place in barley roots. When possible, the interconnections between particular factors are emphasized to draw a broader picture of the molecular mechanisms of drought tolerance in barley.

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Section: Discussionmentioning

confidence: 99%

No Time to Waste: Transcriptome Study Reveals that Drought Tolerance in Barley May Be Attributed to Stressed-Like Expression Patterns that Exist before the Occurrence of Stress

et al. 2018

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“…Because cytokinesis requires new cytoskeleton and cell wall components, it is predictable that the cell wall and cytoskeleton-related proteins would change in response to drought. The cytoskeleton (i.e., microtubules and actin filaments) is a highly dynamic component, which is crucial for cell division, movement, morphogenesis, and signal transduction [168]. In proteomic studies, several cytoskeleton proteins, such as actin, kinesin motor protein, tubulin, profilin, actin depolymerizing factor, and fibrillin were decreased in response to drought stress (Figure 3B).…”

Section: Modulation Of Cell Structure and Cell Cyclementioning

confidence: 99%

“…In proteomic studies, several cytoskeleton proteins, such as actin, kinesin motor protein, tubulin, profilin, actin depolymerizing factor, and fibrillin were decreased in response to drought stress (Figure 3B). The actin genes were also reduced in drought-treated H. vulgare leaves [168]. The decrease of the cytoskeleton and cell cycle-related proteins implies that cell growth is suppressed during drought stress.…”

Section: Modulation Of Cell Structure and Cell Cyclementioning

confidence: 99%

Drought-Responsive Mechanisms in Plant Leaves Revealed by Proteomics

Wang

Cai

et al. 2016

IJMS

209

143

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Plant drought tolerance is a complex trait that requires a global view to understand its underlying mechanism. The proteomic aspects of plant drought response have been extensively investigated in model plants, crops and wood plants. In this review, we summarize recent proteomic studies on drought response in leaves to reveal the common and specialized drought-responsive mechanisms in different plants. Although drought-responsive proteins exhibit various patterns depending on plant species, genotypes and stress intensity, proteomic analyses show that dominant changes occurred in sensing and signal transduction, reactive oxygen species scavenging, osmotic regulation, gene expression, protein synthesis/turnover, cell structure modulation, as well as carbohydrate and energy metabolism. In combination with physiological and molecular results, proteomic studies in leaves have helped to discover some potential proteins and/or metabolic pathways for drought tolerance. These findings provide new clues for understanding the molecular basis of plant drought tolerance.

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“…Chitinase class II (Chi2) and actin isoform B showed 2-sided interaction in DT while both the proteins were not present in DS. Chitinase was detected in more abundance in response to environmental stress in majority crop plants, while actin responds as drought adaptation, by strengthening the cytoskeleton of the stressed tissue [77,78]. The high abundance of beta tubulin to WS suggests the stress-facilitated cytoskeleton remodeling via tubulin in DT.…”

Section: Unique and Newly Induced Proteins To Water Stress Enhancementioning

confidence: 99%

Comparative leaf proteomics of drought-tolerant and -susceptible peanut in response to water stress

Katam

Sakata

Suravajhala³

et al. 2016

Journal of Proteomics

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Peanuts (Arachis hypogaea L.) are a major source of plant protein grown in subtropical and tropical regions of the world. Pre-harvest aflatoxin contamination is a major problem that affects peanut crop yield and food safety. Poor understanding of molecular and cellular mechanisms associated with aflatoxin resistance is largely responsible for the lack of progress in elucidating a process/methodology for reducing aflatoxin contamination in peanuts. Drought perturbs the invasion of the aflatoxin producing fungus and thus affects the quality and yield of peanut. Therefore, more studies involving the effects of drought stress to determine the molecular changes will enhance our understanding of the key metabolic pathways involved in the combined stresses. The changes associated with the biotic and abiotic interactions within the peanut will be used to determine the metabolic pathways involved in the stress tolerance. This research would be beneficial in identifying the tolerant molecular signatures and promoting food safety and consumer health through breeding superior quality peanut cultivars.

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Drought-induced changes in the actin cytoskeleton of barley (Hordeum vulgare L.) leaves

Cited by 25 publications

References 35 publications

No Time to Waste: Transcriptome Study Reveals that Drought Tolerance in Barley May Be Attributed to Stressed-Like Expression Patterns that Exist before the Occurrence of Stress

No Time to Waste: Transcriptome Study Reveals that Drought Tolerance in Barley May Be Attributed to Stressed-Like Expression Patterns that Exist before the Occurrence of Stress

Drought-Responsive Mechanisms in Plant Leaves Revealed by Proteomics

Comparative leaf proteomics of drought-tolerant and -susceptible peanut in response to water stress

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