Different Methods of Acid Phosphatase Immobilization for Its Application in FIA Systems with Potentiometric Detection

Mroczkiewicz, Monika; Bronowska, A.; Pietrzak, Mariusz; Malinowska, Elżbieta

doi:10.1016/j.proeng.2012.09.134

Cited by 2 publications

(2 citation statements)

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“…Moreover, the immobilization of cellulase has been achieved based on physical adsorption, covalent binding, or affinity interactions ( Zang et al, 2014 ; Hosseini et al, 2018 ; Zhang and Hay, 2019 ), including carrier-binding, microemulsion-based organo-gels (MBGs), ultrasonic encapsulation, crosslinking, entrapment, glutathione-labeling, and chelation ( Mroczkiewicz et al, 2012 ; Nicoletti et al, 2015 ). However, enzymes often display drastically lower activity in organic solvents than in water, and the water layer on the molecular surface of enzymes determines their activity in organic media ( Zhang et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Roles of Surfactants in Oriented Immobilization of Cellulase on Nanocarriers and Multiphase Hydrolysis System

et al. 2022

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Surfactants, especially non-ionic surfactants, play an important role in the preparation of nanocarriers and can also promote the enzymatic hydrolysis of lignocellulose. A broad overview of the current status of surfactants on the immobilization of cellulase is provided in this review. In addition, the restricting factors in cellulase immobilization in the complex multiphase hydrolysis system are discussed, including the carrier structure characteristics, solid-solid contact obstacles, external diffusion resistance, limited recycling frequency, and nonproductive combination of enzyme active centers. Furthermore, promising prospects of cellulase-oriented immobilization are proposed, including the hydrophilic-hydrophobic interaction of surfactants and cellulase in the oil-water reaction system, the reversed micelle system of surfactants, and the possible oriented immobilization mechanism.

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Section: Introductionmentioning

confidence: 99%

Roles of Surfactants in Oriented Immobilization of Cellulase on Nanocarriers and Multiphase Hydrolysis System

et al. 2022

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“…Therefore, nanocarriers are widely used in the immobilization of enzymes because of their unique properties, such as large speci c surface area to volume ratio (Cao et al 2016;Malar et al 2020;Roth et al 2016). Moreover, the immobilization of cellulase has been achieved based on physical adsorption, covalent binding, or a nity interactions (Hosseini et al 2018;Zang et al 2014; Zhang and Hay 2019), including carrier-binding, microemulsion-based organo-gels (MBGS), ultrasonic encapsulation, crosslinking, entrapment, glutathione-labeling, and chelation (Mroczkiewicz et al 2012;Nicoletti et al 2015). However, enzymes often display drastically lower activity in organic solvents than in water, and the water layer on the molecular surface of enzymes determines their activity in organic media (Zhang et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Function Of Surfactants In Immobilization of Cellulase And Multiphase Hydrolysis: A Review

Wang

De-yi

Wang

et al. 2021

Preprint

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Surfactants, especially non-ionic surfactants, play an important role in the preparation of nanocarriers and can also promote the enzymatic hydrolysis of lignocellulose. A broad overview of the current status of surfactants on the immobilization of cellulase is provided in this review. In addition, the restricting factors in cellulase immobilization in the complex multiphase hydrolysis system are discussed, including the carrier structure characteristics, solid-solid contact obstacles, external diffusion resistance, limited recycling frequency, and invalid combination of enzyme active centers. Furthermore, promising prospects of cellulase-oriented immobilization are proposed, including the hydrophilic-hydrophobic interaction of surfactants and cellulase in the oil-water reaction system, the reversed micelle system of surfactants, and the possible oriented immobilization mechanism.

show abstract

Different Methods of Acid Phosphatase Immobilization for Its Application in FIA Systems with Potentiometric Detection

Cited by 2 publications

References 5 publications

Roles of Surfactants in Oriented Immobilization of Cellulase on Nanocarriers and Multiphase Hydrolysis System

Roles of Surfactants in Oriented Immobilization of Cellulase on Nanocarriers and Multiphase Hydrolysis System

Function Of Surfactants In Immobilization of Cellulase And Multiphase Hydrolysis: A Review

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