Different hydroxyapatite magnetic nanoparticles for medical imaging: Its effects on hemostatic, hemolytic activity and cellular cytotoxicity

Laranjeira, Marta S.; Moço, Ana; Ferreira, Jorge A.; Coimbra, Susana; Costa, Elı́sio; Santos-Silva, Alice; Ferreira, Paulo J.; Monteiro, Fernando J.

doi:10.1016/j.colsurfb.2016.06.042

Cited by 69 publications

(38 citation statements)

References 28 publications

(33 reference statements)

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“…According to ASTM F756-13 [ 18 ], these particles may be considered non-haemolytic (haemolysis percentage < 2%). Similar results were reported for needle-shape nanoparticles tested in mice erythrocytes at 10 mg/mL [ 10 ] and rod-like nanoparticles assayed in human erythrocytes, up to 4 mg/mL [ 13 ].…”

Section: Discussionsupporting

confidence: 84%

“…In spite of that, biocompatibility of HA has been analysed especially addressing the bone tissue [ 1 ] and information on its interaction with the vascular compartment is scarce and loose. The few studies are mainly concerned with isolated effects on the platelets and/or the coagulation system [ 10 – 13 ]. Others address only the interaction with endothelial cells and, most of them, in a perspective of angiogenesis and bone regeneration [ 14 – 16 ].…”

Section: Introductionmentioning

confidence: 99%

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Vascular biosafety of commercial hydroxyapatite particles: discrepancy between blood compatibility assays and endothelial cell behavior

et al. 2018

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BackgroundVascular homeostasis is ensured by a dynamic interplay involving the endothelium, the platelets and the coagulation system. Thus, the vascular safety of particulate materials must address this integrated system, an approach that has been largely neglected. This work analysed the effects of commercial hydroxyapatite (HA) particles in blood compatibility and in endothelial cell behavior, due to their clinical relevance and scarcity of data on their vascular biosafety.ResultsParticles with similar chemical composition and distinct size and morphology were tested, i.e. rod-like, nano dimensions and low aspect ratio (HAp1) and needle-shape with wider size and aspect ratio (HAp2). HAp1 and HAp2, at 1 to 10 mg/mL, did not affect haemolysis, platelet adhesion, aggregation and activation, or the coagulation system (intrinsic and extrinsic pathways), although HAp2 exhibited a slight thrombogenic potential at 10 mg/mL. Notwithstanding, significantly lower levels presented dose-dependent toxicity on endothelial cells’ behavior. HAp1 and HAp2 decreased cell viability at levels ≥ 250 and ≥ 50 μg/mL, respectively. At 10 and 50 μg/mL, HAp1 did not interfere with the F-actin cytoskeleton, apoptotic index, cell cycle progression, expression of vWF, VECad and CD31, and the ability to form a network of tubular-like structures. Comparatively, HAp2 caused dose-dependent toxic effects in these parameters in the same concentration range.ConclusionThe most relevant observation is the great discrepancy of HA particles’ levels that interfere with the routine blood compatibility assays and the endothelial cell behavior. Further, this difference was also found to be dependent on the particles’ size, morphology and aspect ratio, emphasizing the need of a complementary biological characterization, taking into consideration the endothelial cells’ functionality, to establish the vascular safety of particulate HA.

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Section: Discussionsupporting

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Vascular biosafety of commercial hydroxyapatite particles: discrepancy between blood compatibility assays and endothelial cell behavior

et al. 2018

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“…The study also assessed cytotoxic, hemocompatibility, hemolytic, and thrombogenic effects of various MHAp, of which Gd-doped MHAp was the most biocompatible. 70 Zilm et al reported the wet chemical synthesis of HAp using calcium nitrate (225 mM) and diammonium hydrogen phosphate (80 mM) in a basic (pH 11) condition. The synthesized HAp was further used for an ion exchange procedure to synthesize MHAp.…”

Section: Different Synthesis Routes Of Magnetic Hapmentioning

confidence: 99%

Magnetic hydroxyapatite: a promising multifunctional platform for nanomedicine application

Mondal¹,

Manivasagan²,

Bharathiraja³

et al. 2017

IJN

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In this review, specific attention is paid to the development of nanostructured magnetic hydroxyapatite (MHAp) and its potential application in controlled drug/gene delivery, tissue engineering, magnetic hyperthermia treatment, and the development of contrast agents for magnetic resonance imaging. Both magnetite and hydroxyapatite materials have excellent prospects in nanomedicine with multifunctional therapeutic approaches. To date, many research articles have focused on biomedical applications of nanomaterials because of which it is very difficult to focus on any particular type of nanomaterial. This study is possibly the first effort to emphasize on the comprehensive assessment of MHAp nanostructures for biomedical applications supported with very recent experimental studies. From basic concepts to the real-life applications, the relevant characteristics of magnetic biomaterials are patented which are briefly discussed. The potential therapeutic and diagnostic ability of MHAp-nanostructured materials make them an ideal platform for future nanomedicine. We hope that this advanced review will provide a better understanding of MHAp and its important features to utilize it as a promising material for multifunctional biomedical applications.

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“…The technique allows tomographic observation of structures with high resolution and soft tissue contrast. Nanosized contrast agents have great advantages derived to their high surface area and the capability to be delivered to a specific tumor by appropriate targeting and control over the blood circulation half-life [101,102].…”

Section: Modification Of Hap Nanoparticles and Doping With Fluorescenmentioning

confidence: 99%

Biodegradable and Biocompatible Systems Based on Hydroxyapatite Nanoparticles

et al. 2017

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Abstract:Composites of hydroxyapatite (HAp) are widely employed in biomedical applications due to their biocompatibility, bioactivity and osteoconductivity properties. In fact, the development of industrially scalable hybrids at low cost and high efficiency has a great impact, for example, on bone tissue engineering applications and even as drug delivery systems. New nanocomposites constituted by HAp nanoparticles and synthetic or natural polymers with biodegradable and biocompatible characteristics have constantly been developed and extensive works have been published concerning their applications. The present review is mainly focused on both the capability of HAp nanoparticles to encapsulate diverse compounds as well as the preparation methods of scaffolds incorporating HAp. Attention has also been paid to the recent developments on antimicrobial scaffolds, bioactive membranes, magnetic scaffolds, in vivo imaging systems, hydrogels and coatings that made use of HAp nanoparticles.

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Different hydroxyapatite magnetic nanoparticles for medical imaging: Its effects on hemostatic, hemolytic activity and cellular cytotoxicity

Cited by 69 publications

References 28 publications

Vascular biosafety of commercial hydroxyapatite particles: discrepancy between blood compatibility assays and endothelial cell behavior

Vascular biosafety of commercial hydroxyapatite particles: discrepancy between blood compatibility assays and endothelial cell behavior

Magnetic hydroxyapatite: a promising multifunctional platform for nanomedicine application

Biodegradable and Biocompatible Systems Based on Hydroxyapatite Nanoparticles

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