Different Genospecies of Borrelia burgdorferi Are Associated with Distinct Clinical Manifestations of Lyme Borreliosis

Dam, Alje P. van; Kuiper, H.; Vos, Koen; Widjojokusumo, A.; Jongh, B. M. De; Spanjaard, Lodewijk; Ramselaar, A. C. P.; Kramer, Michael D.; Dankert, J.

doi:10.1093/clinids/17.4.708

Cited by 505 publications

(368 citation statements)

References 20 publications

Supporting

Mentioning

340

Contrasting

Unclassified

Order By: Relevance

“…After long periods of latent infection, 10% of patients may develop chronic neuroborreliosis (33). Neurologic symptoms are seen more frequently in Europe, due to the neurotropism of B garinii, which is isolated mainly in Europe (34).…”

Section: Extracutaneous Manifestations Of Lyme Diseasementioning

confidence: 99%

Ticks, Borrelia Burgdorferi and Lyme Disease

Bağcı¹,

Ruzicka²

2016

tdd

View full text Add to dashboard Cite

Lyme disease, which is caused by spirochete Borrelia burgdorferi sensu lato, is a ticktransmitted, multisystem infectious disease. It occurs in stages, with different clinical manifestations at each stage. Erythema migrans is the most frequent manifestation which occurs at the site of the tick bite. Borrelial lymphocytoma and acrodermatitis chronica atrophicans are late-stage cutaneous manifestations. Extracutaneous signs of infection most often involve the musculoskeletal, cardiovascular, and nervous systems. Serologic assays remain the mainstay of diagnosis. All stages of the disease are curable with appropriate antibiotic therapy. Keywords: Lyme disease, Borrelia burgdorferi, erythema migrans, acrodermatitis chronica atrophicans, ticks, Ixodes Işın Sinem Bağcı, Thomas Ruzicka* Ticks, Borrelia Burgdorferi and Lyme DiseaseKeneler, Borrelia Burgdorferi ve Lyme Hastalığı Öz AbstractLyme hastalığı Borrelia burgdorferi sensu lato grubu spiroketlerin sebep olduğu multisistemik bir enfeksiyon hastalığıdır. Her hastalık evresi değişik klinik bulgularla kendini gösterir. En sık klinik bulgusu kene ısırık yerinde gelişen eritema migranstır. Borrelial lenfositoma ve akrodermatitis kronika atrofikans geç dönemde gelişen deri bulgularıdır. Deri bulguları dışında kas-iskelet, kardiyovasküler ve sinir sistemi tutulumu da görülebilir. Tanı serolojik testler ile konulur. Hastalığın her evresi uygun antibiyotiklerle tedavi edilebilir.

show abstract

Section: Extracutaneous Manifestations Of Lyme Diseasementioning

confidence: 99%

Ticks, Borrelia Burgdorferi and Lyme Disease

Bağcı¹,

Ruzicka²

2016

tdd

View full text Add to dashboard Cite

show abstract

“…Recently, two new American genomic groups were identified on the basis of the results of afla gene-based PCR assay (6). One interesting aspect of the species classification described above is its correlation with epidemiological and clinical features (5,41). B. burgdorferi sensu stricto is present both in the Old World and in the New World, but it seems to be absent from Asia.…”

mentioning

confidence: 99%

“…Moreover, B. burgdorferi, the only Lyme disease Borrelia species known to occur in the United States, is mainly associated with arthritic forms of Lyme disease. B. garinii and B. afielii are present in Europe and Asia; the former is frequently associated with neurological manifestations, and the latter seems to be the exclusive agent of late cutaneous lesions of acrodermatitis chronica atrophicans (Pick-Herxheimer disease), which occurs mainly in northern Europe (13,41,44).…”

mentioning

confidence: 99%

Diversity of Borrelia burgdorfeii Sensu Lato Evidenced by Restriction Fragment Length Polymorphism of rrf (5S)-rrl (23S) Intergenic Spacer Amplicons

Postić

Assous

Grimont

et al. 1994

International Journal of Systematic Bacteriology

490

514

View full text Add to dashboard Cite

The organization of the ribosomal genes is unique in Borrelia burgdogeri in that the rrl (23s) and rrf (5s) genes are tandemly duplicated. We took advantage of this uniqueness to assess the restriction polymorphism of PCR products obtained with primers at the 3' end of the first rrfgene and at the 5' end of the second rrl gene. An amplicon that was 226 to 266 bp long was generated from 99 of 100 B. burgdorferi sensu lato strains. The nuclease MseI restriction polymorphism of the amplicons provided a useful tool for identifying B. burgdorferi sensu stricto, Borrelia garinii, Borrelia afielii (formerly group VS461), and Borrelia japonica (formerly group F63B). Furthermore, it allowed us to recognize four new genomic groups, which were confirmed by DNA-DNA hybridization data. Two of these genomic groups comprised European strains, and the other two groups contained American strains. The American genomic groups involved vectors with enzootic cycles quite different from those of B. burgdorferi sensu stricto, which previously was the only Lyme disease Borrelia species known to occur in the United States. Our method could be used for rapid screening of strain collections and for epidemiological and medical purposes.

show abstract

“…Like many other microorganisms, B. burgdorferi has also developed strategies to resist the serum bactericidal activity, despite the ability of these spirochetes to activate both classical and alternative pathways of C activation (15). Resistance to C-dependent killing varies in different strains of B. burgdorferi (16 -18) and is apparently related to their ability to disseminate to extracutaneous sites (19).…”

mentioning

confidence: 99%

Serum-Resistant Strains of Borrelia burgdorferi Evade Complement-Mediated Killing by Expressing a CD59-Like Complement Inhibitory Molecule

Pausa¹,

Pellis²,

Cinco

et al. 2003

The Journal of Immunology

View full text Add to dashboard Cite

Borrelia burgdorferi, the etiological agent of Lyme disease, comprises three genospecies, Borrelia garinii, afzelii, and burgdorferi sensu strictu, that exhibit different pathogenicity and differ in the susceptibility to C-mediated killing. We examined C-sensitive and C-resistant strains of B. burgdorferi for deposition of C3 and late C components by fluorescence microscope and flow cytometry. Despite comparable deposition of C3 on the two strains, the resistant strain exhibited reduced staining for C6 and C7, barely detectable C9, and undetectable poly C9. Based on these findings, we searched for a protein that inhibits assembly of C membrane attack complex and documented an anti-human CD59-reactive molecule on the surface of C-resistant spirochetes by flow cytometry and electron microscopy. A molecule of 80 kDa recognized by polyclonal and monoclonal anti-CD59 Abs was identified in the membrane extract of C-resistant strains by SDS-PAGE and Western blot analysis. The molecule was released from the bacterial wall using deoxycholate and trypsin, suggesting its insertion into the bacterial membrane. The CD59-like molecule acts as C inhibitor on Borrelia because incubation with F(ab′)2 anti-CD59 renders the serum-resistant strain exquisitely susceptible to C-mediated killing and guinea pig erythrocytes bearing C5b-8, unlike the RBC coated with C5b-7, are protected from reactive lysis by the bacterial extract. Western blot analysis revealed preferential binding of the C inhibitory molecule to C9 and weak interaction with C8β.

show abstract

Different Genospecies of Borrelia burgdorferi Are Associated with Distinct Clinical Manifestations of Lyme Borreliosis

Cited by 505 publications

References 20 publications

Ticks, Borrelia Burgdorferi and Lyme Disease

Ticks, Borrelia Burgdorferi and Lyme Disease

Diversity of Borrelia burgdorfeii Sensu Lato Evidenced by Restriction Fragment Length Polymorphism of rrf (5S)-rrl (23S) Intergenic Spacer Amplicons

Serum-Resistant Strains of Borrelia burgdorferi Evade Complement-Mediated Killing by Expressing a CD59-Like Complement Inhibitory Molecule

Contact Info

Product

Resources

About