Different genomic structure of mouse and human Lmp7 genes: characterization of MHC-encoded proteasome genes

Meinhardt, T.; Gräf, Uwe; Hämmerling, Günter J.

doi:10.1007/bf00210482

Cited by 9 publications

(1 citation statement)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…4 and 5). This motif binds both the activator IRF-1 and repressor IRF-2 transcription factors (40) and is present in the mammalian TAP2, PSMB8, and PSMB10 promoters (32,35,41,42), as well as the bidirectional promoter of the TAP1 and PSMB9 genes (11,12). The zebrafish PSMB9A and PSMB8 genes lack this element.…”

Section: Analysis Of Promoter Regionsmentioning

confidence: 99%

Analysis of a 26-kb Region Linked to theMhcin Zebrafish: Genomic Organization of the Proteasome Component β/Transporter Associated with Antigen Processing-2 Gene Cluster and Identification of Five New Proteasome β Subunit Genes

Murray

Sültmann

Klein

1999

The Journal of Immunology

View full text Add to dashboard Cite

Sequencing of zebrafish (Danio rerio) bacterial artificial chromosome and P1 artificial chromosome genomic clone fragments and of cDNA clones has led to the identification of five new loci coding for β subunits of proteasomes (PSMB). Together with the four genes identified previously, nine PSMB genes have now been defined in the zebrafish. Six of the nine genes reside in the zebrafish MHC (Mhc) class I region, four of them reside in a single cluster closely associated with TAP2 on a 26-kb long genomic fragment, and two reside at some distance from the fragment. In addition to homologues of the human genes PSMB5 through PSMB9, two new genes, PSMB11 and PSMB12, have been found for which there are no known corresponding genes in humans. The new genes reside in the PSMB cluster in the Mhc. Homology and promoter region analysis suggest that the Mhc-associated genes might be inducible by IFN-γ. The zebrafish class I region contains representatives of three phylogenetically distinguishable groups of PSMB genes, X, Y, and Z. It is proposed that these genes were present in the ancestral PSMB region before Mhc class I genes became associated with it.

show abstract

Section: Analysis Of Promoter Regionsmentioning

confidence: 99%

Analysis of a 26-kb Region Linked to theMhcin Zebrafish: Genomic Organization of the Proteasome Component β/Transporter Associated with Antigen Processing-2 Gene Cluster and Identification of Five New Proteasome β Subunit Genes

Murray

Sültmann

Klein

1999

The Journal of Immunology

View full text Add to dashboard Cite

show abstract

Studies on rLMP7, a β-Subunit of the Multicatalytic Proteinase

Ren

Clawson

1997

Experimental Cell Research

View full text Add to dashboard Cite

Divergent intron arrangement in theMB1/LMP7 proteasome gene pair

et al. 1996

View full text Add to dashboard Cite

We sequenced the human MB1 gene from a cosmid clone mapping to chromosome 14q11.2-12. The gene spans about 6 kilobases and contains three exons and two introns. There was no evidence of an alternative leader exon, which is a characteristic of the major histocompatibility complex (MHC)-encoded LMP7 gene, the closest relative of MB1, with which it shares 67% amino acid identity. Conceptual translation of the 5' end of the gene calls for a cleaved leader sequence of 59 amino acids, consistent with western blot data. None of the MB1 gene's three exons were coincident with any of the six exons in LMP7. In contrast, in the delta-encoding gene and its counterpart, the MHC-encoded LMP2 gene (59% amino acid identity), all six exons are arranged at equivalent positions in respect to the coding frame. The unique structure of MB1 implies a separate origin or different selection pressures acting at this particular locus. DNA repeat analysis provides information on the minimum time of separation of the MB1/LMP7 pair of genes.

show abstract

Different genomic structure of mouse and human Lmp7 genes: characterization of MHC-encoded proteasome genes

Cited by 9 publications

References 18 publications

Analysis of a 26-kb Region Linked to theMhcin Zebrafish: Genomic Organization of the Proteasome Component β/Transporter Associated with Antigen Processing-2 Gene Cluster and Identification of Five New Proteasome β Subunit Genes

Analysis of a 26-kb Region Linked to theMhcin Zebrafish: Genomic Organization of the Proteasome Component β/Transporter Associated with Antigen Processing-2 Gene Cluster and Identification of Five New Proteasome β Subunit Genes

Studies on rLMP7, a β-Subunit of the Multicatalytic Proteinase

Divergent intron arrangement in theMB1/LMP7 proteasome gene pair

Contact Info

Product

Resources

About