Different enzymes involved in NADH- and NADPH-dependent respiration in the cyanobacterium Anabaena variabilis

Interaction of photosynthesis, respiration and nitrogen fixation in cyanobacteria

“…NADPH has been suggested to be oxidized by FNR in heterocyst preparations producing reduced ferredoxin (Houchins andHind 1982, Schrautemeier et al 1985). Interestingly, NADPH oxidation activity of a membrane preparation of vegetative cells of Anabaena can be destroyed by washing membranes without any detergent while NADH oxidation remains intact (Table 1); NADPH oxidation is inhibited by NADP ÷ , which is a feature characteristic for the FNR (Alpes et al 1985). The same was found for oxidative phosphorylation, driven by either NADPH or NADH (Scherer et al, in preparation).…”

Partial conservation of the 5? ndhE-psaC-ndhD 3? gene arrangement of chloroplasts in the cyanobacterium Synechocystis sp. PCC 6803: implications for NDH-D function in cyanobacteria and chloroplasts

“…In Anabaena variabilis ATCC 29413 N A D P H oxidation is performed by ferredoxin-NADP + oxidoreductase, whereas N A D H oxidation is performed by a separate N A D H dehydrogenase [2]. The thylakoid-bound respiratory N A D H dehydrogenase isolated from Anabaena 29413 is more similar to bacterial than to mitochondrial N A D H dehydrogenases on the basis of subunit number, sensitivity to inhibitors, and prosthetic group [ 1 ].…”

Cyanobacterial Respiration

“…Schrautemeier et al (1985) found that membranes from Anabaena variabilis heterocysts washed free of FNR had lost their ability to use NADPH as the electron donor to Photosystem I. Washing membranes from Anabaena variabilis also inhibited NADPH-dependent oxygen uptake (Alpes et al, 1985) and NADPH-dependent oxidative phosphorylation (Schrautemeier et al, 1988). FNR constituted the onlyNADPH-menadione oxidoreductase found in Anabaena variabilis.…”