Cyanobacterial Respiration

Schmetterer, Georg

doi:10.1007/0-306-48205-3_13

Cited by 44 publications

(59 citation statements)

References 192 publications

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“…We could identify NdhF3 and protein Tll1812, homologous to a potential NADH dehydrogenase subunit (Wang et al, 2004;Daley et al, 2012), as candidates for binding partners. Interestingly, this would also be in agreement with the formation of a supercomplex between b 6 f and NDH-1 with a potential role in cyclic (Ohkawa et al, 2000;Battchikova et al, 2011) and respiratory electron transport (Schmetterer, 1994). As recently observed for NDH-1 and succinate dehydrogenase, binding of PetP to b 6 f and NDH-1 could induce redistribution of these complexes, combined with changes in their electron transport activity (Liu et al, 2012).…”

Section: Suggested Role Of Petp In Cyanobacterial Electron Transportsupporting

confidence: 87%

Functional Characterization of the Small Regulatory Subunit PetP from the Cytochrome b₆f Complex in Thermosynechococcus elongatus

Rexroth

Veit

et al. 2014

Plant Cell

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The cyanobacterial cytochrome b 6 f complex is central for the coordination of photosynthetic and respiratory electron transport and also for the balance between linear and cyclic electron transport. The development of a purification strategy for a highly active dimeric b 6 f complex from the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1 enabled characterization of the structural and functional role of the small subunit PetP in this complex. Moreover, the efficient transformability of this strain allowed the generation of a DpetP mutant. Analysis on the whole-cell level by growth curves, photosystem II light saturation curves, and P700 + reduction kinetics indicate a strong decrease in the linear electron transport in the mutant strain versus the wild type, while the cyclic electron transport via photosystem I and cytochrome b 6 f is largely unaffected. This reduction in linear electron transport is accompanied by a strongly decreased stability and activity of the isolated DpetP complex in comparison with the dimeric wild-type complex, which binds two PetP subunits. The distinct behavior of linear and cyclic electron transport may suggest the presence of two distinguishable pools of cytochrome b 6 f complexes with different functions that might be correlated with supercomplex formation.

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Section: Suggested Role Of Petp In Cyanobacterial Electron Transportsupporting

confidence: 87%

Functional Characterization of the Small Regulatory Subunit PetP from the Cytochrome b₆f Complex in Thermosynechococcus elongatus

Rexroth

Veit

et al. 2014

Plant Cell

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“…Therefore, the redox status of electron transport components (i.e. the PQ pool, the cytochrome b 6 f complex and plastocyanin) depend on photosynthetic light harvesting and on the metabolism of respiratory substrates [48,49]. Thus, the redox state of the PQ pool more or less reflects the energy state of the cell.…”

Section: Trends In Plantmentioning

confidence: 99%

Chloroplast redox signals: how photosynthesis controls its own genes

Pfannschmidt

2003

Trends in Plant Science

444

301

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“…To see if Glc induction of respiratory gene expression is also mediated by these changes, we investigated the expression levels of 18 genes in wildtype cells treated with chemical inhibitors of the OPP pathway and oxidative phosphorylation, such as 6-aminonicotinamide (6-AN, G6PDH inhibitor; Varnes, 1988), Hg 21 (NDH-1 inhibitor; Mi et al, 1992), CN 2 (CtaI inhibitor), and carbonylcyanide-3-chlorophenyl hydrazone (CCCP; an uncoupler; Schmetterer, 1994). Dark-adapted Synechocystis cells were pretreated with inhibitors for 3 min before treating them with 10 mM Glc for 2, 8, and 20 min in the dark.…”

Section: Analysis Of Transcript Levels By Rt-pcrmentioning

confidence: 99%

“…In cyanobacteria as well as in other organisms, respiratory electron transport is coupled to proton translocation (Schmetterer, 1994), which results in an increase in the cytosolic pH as shown by the Glcinduced increase in acridine yellow (AY) fluorescence (Ryu et al, 2004). In Synechocystis, cytosolic alkalization is responsible for Glc-induced expression of carotenoid biosynthesis genes (Ryu et al, 2004).…”

Section: Analysis Of Transcript Levels By Rt-pcrmentioning

confidence: 99%

Transcriptional Regulation of the Respiratory Genes in the CyanobacteriumSynechocystissp. PCC 6803 during the Early Response to Glucose Feeding

et al. 2007

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The coordinated expression of the genes involved in respiration in the photosynthetic cyanobacterium Synechocystis sp. PCC 6803 during the early period of glucose (Glc) treatment is poorly understood. When photoautotrophically grown cells were supplemented with 10 mM Glc in the light or after a dark adaptation period of 14 h, significant increases in the respiratory activity, as determined by NAD(P)H turnover, respiratory O 2 uptake rate, and cytosolic alkalization, were observed. At the same time, the transcript levels of 18 genes coding for enzymes associated with respiration increased with differential induction kinetics; these genes were classified into three groups based on their half-rising times. Transcript levels of the four genes gpi, zwf, pdhB, and atpB started to increase along with a net increase in NAD(P)H, while the onset of net NAD(P)H consumption coincided with an increase in those of the genes tktA, ppc, pdhD, icd, ndhD2, ndbA, ctaD1, cydA, and atpE. In contrast, the expression of the atpI/G/D/A/C genes coding for ATP synthase subunits was the slowest among respiratory genes and their expression started to accumulate only after the establishment of cytosolic alkalization. These differential effects of Glc on the transcript levels of respiratory genes were not observed by inactivation of the genes encoding the Glc transporter or glucokinase. In addition, several Glc analogs could not mimic the effects of Glc. Our findings suggest that genes encoding some enzymes involved in central carbon metabolism and oxidative phosphorylation are coordinately regulated at the transcriptional level during the switch of nutritional mode.Respiration releases the energy stored in carbon compounds and also provides many carbon precursors for the biosynthesis of a wide variety of plant biomolecules, including amino acids, lipids, isoprenoids, and porphyrins. D-Glc (hereafter Glc) is an immediate substrate for the four major respiration processes: glycolysis, the oxidative pentose phosphate (OPP) pathway, the tricarboxylic acid (TCA) cycle, and oxidative phosphorylation. In plants, respiration is predominantly controlled by the key metabolites ADP and inorganic phosphate, and the ratio of NADPH to NADP 1 . The cellular concentration of ADP initially controls the rates of electron transfer and ATP synthesis, which in turn affect TCA cycle activity, finally resulting in the regulation of glycolytic reactions.

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Cyanobacterial Respiration

Cited by 44 publications

References 192 publications

Functional Characterization of the Small Regulatory Subunit PetP from the Cytochrome b₆f Complex in Thermosynechococcus elongatus

Functional Characterization of the Small Regulatory Subunit PetP from the Cytochrome b₆f Complex in Thermosynechococcus elongatus

Chloroplast redox signals: how photosynthesis controls its own genes

Transcriptional Regulation of the Respiratory Genes in the CyanobacteriumSynechocystissp. PCC 6803 during the Early Response to Glucose Feeding

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Cyanobacterial Respiration

Cited by 44 publications

References 192 publications

Functional Characterization of the Small Regulatory Subunit PetP from the Cytochrome b6f Complex in Thermosynechococcus elongatus

Functional Characterization of the Small Regulatory Subunit PetP from the Cytochrome b6f Complex in Thermosynechococcus elongatus

Chloroplast redox signals: how photosynthesis controls its own genes

Transcriptional Regulation of the Respiratory Genes in the CyanobacteriumSynechocystissp. PCC 6803 during the Early Response to Glucose Feeding

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Functional Characterization of the Small Regulatory Subunit PetP from the Cytochrome b₆f Complex in Thermosynechococcus elongatus

Functional Characterization of the Small Regulatory Subunit PetP from the Cytochrome b₆f Complex in Thermosynechococcus elongatus