Different effects of partial pressure in a high-pressure gaseous mixture of carbon monoxide and oxygen for rat heart preservation

Hatayama, Naoyuki; Hirai, Shuichi; Fukushige, Kaori; Yokota, Hiroki; Itoh, Masahiro; Naito, Makoto

doi:10.1038/s41598-019-43905-0

Cited by 3 publications

(3 citation statements)

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“…Heterotopic cervical heart transplantation to a recipient rat was performed for morphological and functional analyses, as described previously [ 8 ]. At 60 min following the heterotopic cervical heart transplantation, the heartbeat was counted.…”

Section: Methodsmentioning

confidence: 99%

“…For the HPG preservation method, a chamber that could withstand high pressure was developed, as previously described (Nakamura Iron Works Co., Ltd. Tokyo, Japan) [8]. To prepare for organ preservation, the inne chamber was placed in distilled water to maintain humidity and then cooled to 4 • C. Extracted rat hearts suspended in a plastic cylinder were placed in the chamber, and the lid was closed using four bolts.…”

Section: Hpg Preservation Methods and Experimental Designmentioning

confidence: 99%

“…A previous report demonstrated that a high-pressure gas (HPG) preservation method using a mixture of CO and O 2 gases successfully resuscitated extracted rat hearts after 48 h of preservation [ 7 ]. Rat hearts exposed to hyperbaric CO and O 2 without immersion in cold storage (CS) solution has been found to effectively obtain necessary cell-protective effects of gas for preservation [ 8 ]. The rat heart function after 24 h of HPG preservation using a mixture of CO and O 2 gases was also found to be almost the same as that in control hearts that were transplanted immediately after extraction [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

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Cardioprotection via Metabolism for Rat Heart Preservation Using the High-Pressure Gaseous Mixture of Carbon Monoxide and Oxygen

Suzuki

Hatayama

Ogawa

et al. 2020

IJMS

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The high-pressure gas (HPG) method with carbon monoxide (CO) and oxygen (O2) mixture maintains the preserved rat heart function. The metabolites of rat hearts preserved using the HPG method (HPG group) and cold storage (CS) method (CS group) by immersion in a stock solution for 24 h were assessed to confirm CO and O2 effects. Lactic acid was significantly lower and citric acid was significantly higher in the HPG group than in the CS group. Moreover, adenosine triphosphate (ATP) levels as well as some pentose phosphate pathway (PPP) metabolites and reduced nicotinamide adenine dinucleotide phosphate (NADPH) were significantly higher in the HPG group than in the CS group. Additionally, reduced glutathione (GSH), which protects cells from oxidative stress, was also significantly higher in the HPG group than in the CS group. These results indicated that each gas, CO and O2, induced the shift from anaerobic to aerobic metabolism, maintaining the energy of ischemic preserved organs, shifting the glucose utilization from glycolysis toward PPP, and reducing oxidative stress. Both CO and O2 in the HPG method have important effects on the ATP supply and decrease oxidative stress for preventing ischemic injury. The HPG method may be useful for clinical application.

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Section: Methodsmentioning

confidence: 99%

Section: Hpg Preservation Methods and Experimental Designmentioning

confidence: 99%