The Effect of Pressure of Gas Mixtures Containing Carbon Monoxide, Oxygen and Argon on the Shelf Life of Rat Heart Tissues in Hypothermic Conditions

Gurin, A. E.; Gagarinsky, E. L.; Фесенко, Е. Е.

doi:10.1134/s0006350921050079

Cited by 1 publication

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“…In previous research by Gurin et. al., [ 40 ] it was assumed that the protective effect of oxygen was due to the maintenance of a reduced, but stable level of aerobic metabolism during hypothermic storage. Maintenance of the latter does not allow cells to switch to anaerobic respiration, accompanied by accumulation of lactic acid, a decrease in ATP production, which would normally result in a failure of ATP dependent Na/K pumps and Ca pumps follows by osmotic swelling of the cell and eventual lysis.…”

Section: Discussionmentioning

confidence: 99%

Prolonged hypothermic storage of oocytes of the European common frog Rana temporaria in a gas mixture of oxygen and carbon monoxide

2023

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The maximum hypothermic storage time of amphibian oocytes is several hours, which is due to the peculiarities of the structure of the cell envelope. The authors of this paper have already demonstrated the possibility of increasing the storage period of unfertilized oocytes of the common frog (Rana temporaria) up to 5–7 days. The aim of the current study was to determine the possibility of using a 6.5 atm gaseous mixture of carbon monoxide and oxygen, for prolonged hypothermic preservation of unfertilized oocytes for 4 to 12 days. After four days, oocytes stored under CO+O2 conditions exhibited fertilization and hatching rates that were 1.6 and 2.2-fold higher than control, respectively. While no oocytes in the control group survived to day twelve, oocytes held under CO +O2 gas exhibited a 39±14% (38 out of 99 oocytes in total) fertilization rate, however only 1±2% (1/99) of those hatched. This approach is promising for the storage of genetic material from female amphibians, particularly in respect to managing and restoring endangered species, but may also be applicable to oocytes of other classes of vertebrates.

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Section: Discussionmentioning

confidence: 99%